Online simultaneous determination of lactoferrin and immunoglobulin G in dairy products by two-dimensional liquid chromatography
ObjectiveA method of two-dimensional liquid chromatograohy was established to simultaneously determine the content of lactoferrin and immunoglobulin G in dairy products.MethodsThe pretreated samples were directly injected into the constructed system. Target protein and other effects were separated o...
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The Editorial Office of Food and Machinery
2024-09-01
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| Series: | Shipin yu jixie |
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| Online Access: | http://www.ifoodmm.com/spyjx/article/abstract/20240909?st=article_issue |
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| author | CHEN Qin ZHOU Langjun CHEN Zijing LIU Mengni LIN Senyu |
| author_facet | CHEN Qin ZHOU Langjun CHEN Zijing LIU Mengni LIN Senyu |
| author_sort | CHEN Qin |
| collection | DOAJ |
| description | ObjectiveA method of two-dimensional liquid chromatograohy was established to simultaneously determine the content of lactoferrin and immunoglobulin G in dairy products.MethodsThe pretreated samples were directly injected into the constructed system. Target protein and other effects were separated on a Pharmacia HI-Trap Protein G as the first-dimensional column using a mobile phase consisting of sodium phosphate buffer and glycocoll buffer by gradient elution lactoferrin and immunoglobulin G were separated on a BioCore SEC-300 (4.6 mm×250 mm, 5 μm) as the second-dimensional column using a mobile phase consisting of water and sodium phosphate buffer by gradient elution.ResultsThe results showed that the standard curves of Lactoferrin and Immunoglobulin G were linear with R>0.99. The quantitation limits of Lactoferrin in solid samples was 20 mg/kg while in liquid samples was 13 mg/kg, the recovery rate were 91.0%~107.0% and the RSD were 2.4%~5.3%. The quantitation limits of Immunoglobulin G in solid samples was 34 mg/kg while in liquid samples was 7 mg/kg, the recovery rate were 91.2%~104.0% and the RSD were 2.1%~4.7%.ConclusionThe online two-dimensional liquid chromatography system established in the experiment has the advantages of simple pretreatment, high automation, high analysis efficiency, high accuracy and good repeatability, and is suitable for the determination of lactoferrin and immunoglobulin G in dairy products. |
| format | Article |
| id | doaj-art-fc6eeb145fda47da8f48a0ae6cec864b |
| institution | OA Journals |
| issn | 1003-5788 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | The Editorial Office of Food and Machinery |
| record_format | Article |
| series | Shipin yu jixie |
| spelling | doaj-art-fc6eeb145fda47da8f48a0ae6cec864b2025-08-20T02:04:52ZengThe Editorial Office of Food and MachineryShipin yu jixie1003-57882024-09-01409616510.13652/j.spjx.1003.5788.2023.811091003-5788（2024）09-0061-05Online simultaneous determination of lactoferrin and immunoglobulin G in dairy products by two-dimensional liquid chromatographyCHEN Qin0ZHOU Langjun1CHEN Zijing2LIU Mengni3LIN Senyu4Guangzhou Inspection Testing and Certification Group Company Limited, Guangzhou, Guangdong511447, ChinaGuangzhou Inspection Testing and Certification Group Company Limited, Guangzhou, Guangdong511447, ChinaGuangzhou Inspection Testing and Certification Group Company Limited, Guangzhou, Guangdong511447, ChinaGuangzhou Inspection Testing and Certification Group Company Limited, Guangzhou, Guangdong511447, ChinaGuangzhou Inspection Testing and Certification Group Company Limited, Guangzhou, Guangdong511447, ChinaObjectiveA method of two-dimensional liquid chromatograohy was established to simultaneously determine the content of lactoferrin and immunoglobulin G in dairy products.MethodsThe pretreated samples were directly injected into the constructed system. Target protein and other effects were separated on a Pharmacia HI-Trap Protein G as the first-dimensional column using a mobile phase consisting of sodium phosphate buffer and glycocoll buffer by gradient elution lactoferrin and immunoglobulin G were separated on a BioCore SEC-300 (4.6 mm×250 mm, 5 μm) as the second-dimensional column using a mobile phase consisting of water and sodium phosphate buffer by gradient elution.ResultsThe results showed that the standard curves of Lactoferrin and Immunoglobulin G were linear with R>0.99. The quantitation limits of Lactoferrin in solid samples was 20 mg/kg while in liquid samples was 13 mg/kg, the recovery rate were 91.0%~107.0% and the RSD were 2.4%~5.3%. The quantitation limits of Immunoglobulin G in solid samples was 34 mg/kg while in liquid samples was 7 mg/kg, the recovery rate were 91.2%~104.0% and the RSD were 2.1%~4.7%.ConclusionThe online two-dimensional liquid chromatography system established in the experiment has the advantages of simple pretreatment, high automation, high analysis efficiency, high accuracy and good repeatability, and is suitable for the determination of lactoferrin and immunoglobulin G in dairy products.http://www.ifoodmm.com/spyjx/article/abstract/20240909?st=article_issuelactoferrinimmunoglobulin gonline two-dimensional liquid chromatographydairy products |
| spellingShingle | CHEN Qin ZHOU Langjun CHEN Zijing LIU Mengni LIN Senyu Online simultaneous determination of lactoferrin and immunoglobulin G in dairy products by two-dimensional liquid chromatography Shipin yu jixie lactoferrin immunoglobulin g online two-dimensional liquid chromatography dairy products |
| title | Online simultaneous determination of lactoferrin and immunoglobulin G in dairy products by two-dimensional liquid chromatography |
| title_full | Online simultaneous determination of lactoferrin and immunoglobulin G in dairy products by two-dimensional liquid chromatography |
| title_fullStr | Online simultaneous determination of lactoferrin and immunoglobulin G in dairy products by two-dimensional liquid chromatography |
| title_full_unstemmed | Online simultaneous determination of lactoferrin and immunoglobulin G in dairy products by two-dimensional liquid chromatography |
| title_short | Online simultaneous determination of lactoferrin and immunoglobulin G in dairy products by two-dimensional liquid chromatography |
| title_sort | online simultaneous determination of lactoferrin and immunoglobulin g in dairy products by two dimensional liquid chromatography |
| topic | lactoferrin immunoglobulin g online two-dimensional liquid chromatography dairy products |
| url | http://www.ifoodmm.com/spyjx/article/abstract/20240909?st=article_issue |
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