The histone methyltransferase DOT1B is dispensable for stage differentiation and macrophage infection of Leishmania mexicana
Conserved histone methyltransferases of the DOT1 family are involved in replication regulation, cell cycle progression, stage differentiation, and gene regulation in trypanosomatids. However, the specific functions of these enzymes depend on the host evasion strategies of the parasites. In this stud...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1502339/full |
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| author | Nicole Eisenhuth Elisa Theres Rauh Melina Mitnacht Andrea Debus Ulrike Schleicher Falk Butter Katerina Pruzinova Petr Volf Christian J. Janzen |
| author_facet | Nicole Eisenhuth Elisa Theres Rauh Melina Mitnacht Andrea Debus Ulrike Schleicher Falk Butter Katerina Pruzinova Petr Volf Christian J. Janzen |
| author_sort | Nicole Eisenhuth |
| collection | DOAJ |
| description | Conserved histone methyltransferases of the DOT1 family are involved in replication regulation, cell cycle progression, stage differentiation, and gene regulation in trypanosomatids. However, the specific functions of these enzymes depend on the host evasion strategies of the parasites. In this study, we investigated the role of DOT1B in Leishmania mexicana, focusing on life cycle progression and infectivity. In contrast to Trypanosoma brucei, in which DOT1B is essential for the differentiation of mammal-infective bloodstream forms to insect procyclic forms, L. mexicana DOT1B (LmxDOT1B) is not critical for the differentiation of promastigotes to amastigotes in vitro. Additionally, there are no significant differences in the ability to infect or differentiate in macrophages or sand fly vectors between the LmxDOT1B-depleted and control strains. These findings highlight the divergence of the function of DOT1B in these related parasites, suggesting genus-specific adaptations in the use of histone modifications for life cycle progression and host adaptation processes. |
| format | Article |
| id | doaj-art-fc56401b15684082a3feeec78859b9f8 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-fc56401b15684082a3feeec78859b9f82025-01-20T05:23:49ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011410.3389/fcimb.2024.15023391502339The histone methyltransferase DOT1B is dispensable for stage differentiation and macrophage infection of Leishmania mexicanaNicole Eisenhuth0Elisa Theres Rauh1Melina Mitnacht2Andrea Debus3Ulrike Schleicher4Falk Butter5Katerina Pruzinova6Petr Volf7Christian J. Janzen8Department of Cell and Developmental Biology, Biocenter, University of Würzburg, Würzburg, GermanyDepartment of Cell and Developmental Biology, Biocenter, University of Würzburg, Würzburg, GermanyDepartment of Cell and Developmental Biology, Biocenter, University of Würzburg, Würzburg, GermanyMicrobiology Institute-Clinical Microbiology, Immunology and Hygiene, University Hospital Erlangen and Friedrich-Alexander-University, Erlangen, GermanyMicrobiology Institute-Clinical Microbiology, Immunology and Hygiene, University Hospital Erlangen and Friedrich-Alexander-University, Erlangen, GermanyProteomics und Systems Biology, Institute of Molecular Virology and Cell Biology (IMVZ), Friedrich Loeffler Institute, Greifswald, GermanyDepartment of Parasitology, Charles University, Prague, CzechiaDepartment of Parasitology, Charles University, Prague, CzechiaDepartment of Cell and Developmental Biology, Biocenter, University of Würzburg, Würzburg, GermanyConserved histone methyltransferases of the DOT1 family are involved in replication regulation, cell cycle progression, stage differentiation, and gene regulation in trypanosomatids. However, the specific functions of these enzymes depend on the host evasion strategies of the parasites. In this study, we investigated the role of DOT1B in Leishmania mexicana, focusing on life cycle progression and infectivity. In contrast to Trypanosoma brucei, in which DOT1B is essential for the differentiation of mammal-infective bloodstream forms to insect procyclic forms, L. mexicana DOT1B (LmxDOT1B) is not critical for the differentiation of promastigotes to amastigotes in vitro. Additionally, there are no significant differences in the ability to infect or differentiate in macrophages or sand fly vectors between the LmxDOT1B-depleted and control strains. These findings highlight the divergence of the function of DOT1B in these related parasites, suggesting genus-specific adaptations in the use of histone modifications for life cycle progression and host adaptation processes.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1502339/fullhistone methyltransferaseDOT1Leishmania mexicanavirulencedifferentiationsand fly |
| spellingShingle | Nicole Eisenhuth Elisa Theres Rauh Melina Mitnacht Andrea Debus Ulrike Schleicher Falk Butter Katerina Pruzinova Petr Volf Christian J. Janzen The histone methyltransferase DOT1B is dispensable for stage differentiation and macrophage infection of Leishmania mexicana Frontiers in Cellular and Infection Microbiology histone methyltransferase DOT1 Leishmania mexicana virulence differentiation sand fly |
| title | The histone methyltransferase DOT1B is dispensable for stage differentiation and macrophage infection of Leishmania mexicana |
| title_full | The histone methyltransferase DOT1B is dispensable for stage differentiation and macrophage infection of Leishmania mexicana |
| title_fullStr | The histone methyltransferase DOT1B is dispensable for stage differentiation and macrophage infection of Leishmania mexicana |
| title_full_unstemmed | The histone methyltransferase DOT1B is dispensable for stage differentiation and macrophage infection of Leishmania mexicana |
| title_short | The histone methyltransferase DOT1B is dispensable for stage differentiation and macrophage infection of Leishmania mexicana |
| title_sort | histone methyltransferase dot1b is dispensable for stage differentiation and macrophage infection of leishmania mexicana |
| topic | histone methyltransferase DOT1 Leishmania mexicana virulence differentiation sand fly |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1502339/full |
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