Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes
A high percentage of type 2 diabetes mellitus (T2D) patients are also affected by dyslipidemia and chronic periodontitis (CP), but no studies have determined the gene expression in patients that are simultaneously affected by all three diseases. We investigated the systemic expression of immune-rela...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/1491405 |
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| author | Rafael Nepomuceno Bárbara Scoralick Villela Sâmia Cruz Tfaile Corbi Alliny De Souza Bastos Raquel Alves Dos Santos Catarina Satie Takahashi Silvana Regina Perez Orrico Raquel Mantuaneli Scarel-Caminaga |
| author_facet | Rafael Nepomuceno Bárbara Scoralick Villela Sâmia Cruz Tfaile Corbi Alliny De Souza Bastos Raquel Alves Dos Santos Catarina Satie Takahashi Silvana Regina Perez Orrico Raquel Mantuaneli Scarel-Caminaga |
| author_sort | Rafael Nepomuceno |
| collection | DOAJ |
| description | A high percentage of type 2 diabetes mellitus (T2D) patients are also affected by dyslipidemia and chronic periodontitis (CP), but no studies have determined the gene expression in patients that are simultaneously affected by all three diseases. We investigated the systemic expression of immune-related genes in T2D, dyslipidemia, and CP patients. One hundred and fifty patients were separated into five groups containing 30 individuals each: (G1) poorly controlled T2D with dyslipidemia and CP; (G2) well-controlled T2D with dyslipidemia and CP; (G3) normoglycemic individuals with dyslipidemia and CP; (G4) healthy individuals with CP; (G5) systemic and periodontally healthy individuals. Blood analyses of lipid and glycemic profiles were carried out. The expression of genes, including IL10, JAK1, STAT3, SOCS3, IP10, ICAM1, IFNA, IFNG, STAT1, and IRF1, was investigated by RT-qPCR. Patients with dyslipidemia demonstrated statistically higher expression of the IL10 and IFNA genes, while IFNG, IP10, IRF1, JAK1, and STAT3 were lower in comparison with nondyslipidemic patients. Anti-inflammatory genes, such as IL10, positively correlated with parameters of glucose, lipid, and periodontal profiles, while proinflammatory genes, such as IFNG, were negatively correlated with these parameters. We conclude that dyslipidemia appears to be the primary disease that is associated with gene expression of immune-related genes, while parameters of T2D and CP were correlated with the expression of these important immune genes. |
| format | Article |
| id | doaj-art-fbf88dc2283043d79c63987c742ce045 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-fbf88dc2283043d79c63987c742ce0452025-02-03T01:32:29ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/14914051491405Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory GenesRafael Nepomuceno0Bárbara Scoralick Villela1Sâmia Cruz Tfaile Corbi2Alliny De Souza Bastos3Raquel Alves Dos Santos4Catarina Satie Takahashi5Silvana Regina Perez Orrico6Raquel Mantuaneli Scarel-Caminaga7Department of Morphology, School of Dentistry at Araraquara, São Paulo State University (UNESP), 14801-903 Araraquara, SP, BrazilDepartment of Morphology, School of Dentistry at Araraquara, São Paulo State University (UNESP), 14801-903 Araraquara, SP, BrazilDepartment of Morphology, School of Dentistry at Araraquara, São Paulo State University (UNESP), 14801-903 Araraquara, SP, BrazilDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University (UNESP), 14801-903 Araraquara, SP, BrazilPostgraduate Program in Sciences of the University of Franca, 14404-600 Franca, SP, BrazilDepartment of Genetics, Faculty of Medicine of Ribeirão Preto, University of São Paulo (USP), 14040-900 Ribeirão Preto, SP, BrazilDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, São Paulo State University (UNESP), 14801-903 Araraquara, SP, BrazilDepartment of Morphology, School of Dentistry at Araraquara, São Paulo State University (UNESP), 14801-903 Araraquara, SP, BrazilA high percentage of type 2 diabetes mellitus (T2D) patients are also affected by dyslipidemia and chronic periodontitis (CP), but no studies have determined the gene expression in patients that are simultaneously affected by all three diseases. We investigated the systemic expression of immune-related genes in T2D, dyslipidemia, and CP patients. One hundred and fifty patients were separated into five groups containing 30 individuals each: (G1) poorly controlled T2D with dyslipidemia and CP; (G2) well-controlled T2D with dyslipidemia and CP; (G3) normoglycemic individuals with dyslipidemia and CP; (G4) healthy individuals with CP; (G5) systemic and periodontally healthy individuals. Blood analyses of lipid and glycemic profiles were carried out. The expression of genes, including IL10, JAK1, STAT3, SOCS3, IP10, ICAM1, IFNA, IFNG, STAT1, and IRF1, was investigated by RT-qPCR. Patients with dyslipidemia demonstrated statistically higher expression of the IL10 and IFNA genes, while IFNG, IP10, IRF1, JAK1, and STAT3 were lower in comparison with nondyslipidemic patients. Anti-inflammatory genes, such as IL10, positively correlated with parameters of glucose, lipid, and periodontal profiles, while proinflammatory genes, such as IFNG, were negatively correlated with these parameters. We conclude that dyslipidemia appears to be the primary disease that is associated with gene expression of immune-related genes, while parameters of T2D and CP were correlated with the expression of these important immune genes.http://dx.doi.org/10.1155/2017/1491405 |
| spellingShingle | Rafael Nepomuceno Bárbara Scoralick Villela Sâmia Cruz Tfaile Corbi Alliny De Souza Bastos Raquel Alves Dos Santos Catarina Satie Takahashi Silvana Regina Perez Orrico Raquel Mantuaneli Scarel-Caminaga Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes Mediators of Inflammation |
| title | Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes |
| title_full | Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes |
| title_fullStr | Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes |
| title_full_unstemmed | Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes |
| title_short | Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes |
| title_sort | dyslipidemia rather than type 2 diabetes mellitus or chronic periodontitis affects the systemic expression of pro and anti inflammatory genes |
| url | http://dx.doi.org/10.1155/2017/1491405 |
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