TESTING SHEEP AND GOAT POX VIRUSES FOR THEIR REPRODUCTION IN PRIMARY AND SUBCULTURED CELLS
Results of tests of sheep and goat poxviruses for their reproduction in primary and subcultured cell cultures derived from lamb and goat kid kidneys and testicles are presented. Monolayer cultures were subcultured by 5 passages in plastic vials and infected with sheep and goat poxviruses. It was sho...
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| Format: | Article |
| Language: | English |
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Da Vinci Media
2019-06-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/413 |
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| author | A. B. Sarbasov B. L. Manin R. V. Yashin I. N. Shumilova V. I. Diev |
| author_facet | A. B. Sarbasov B. L. Manin R. V. Yashin I. N. Shumilova V. I. Diev |
| author_sort | A. B. Sarbasov |
| collection | DOAJ |
| description | Results of tests of sheep and goat poxviruses for their reproduction in primary and subcultured cell cultures derived from lamb and goat kid kidneys and testicles are presented. Monolayer cultures were subcultured by 5 passages in plastic vials and infected with sheep and goat poxviruses. It was shown that production ARRIAH strain of sheep pox virus and ARRIAH 2003 strain of goat pox virus successfully propagated both in primary lamb and goat kid kidney and testicle cell cultures and lamb and goat kid kidney and testicle cell subcultures. Activity of sheep and goat poxviruses passaged 5 times was 5.5–6.0 lg TCID50/cm3. Taking into account that modern cell cultivation conditions allow primary trypsinized cell populations subcultivation up to 25–30th passage, subcultures together with continuous cell lines can be used for large-scale sheep and goat poxvirus production and research purposes. |
| format | Article |
| id | doaj-art-fbf5ffcb89b447a3b07d082d5ee16438 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2019-06-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-fbf5ffcb89b447a3b07d082d5ee164382025-02-06T09:52:07ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592019-06-0102354010.29326/2304-196X-2019-2-29-35-40406TESTING SHEEP AND GOAT POX VIRUSES FOR THEIR REPRODUCTION IN PRIMARY AND SUBCULTURED CELLSA. B. Sarbasov0B. L. Manin1R. V. Yashin2I. N. Shumilova3V. I. DievFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirResults of tests of sheep and goat poxviruses for their reproduction in primary and subcultured cell cultures derived from lamb and goat kid kidneys and testicles are presented. Monolayer cultures were subcultured by 5 passages in plastic vials and infected with sheep and goat poxviruses. It was shown that production ARRIAH strain of sheep pox virus and ARRIAH 2003 strain of goat pox virus successfully propagated both in primary lamb and goat kid kidney and testicle cell cultures and lamb and goat kid kidney and testicle cell subcultures. Activity of sheep and goat poxviruses passaged 5 times was 5.5–6.0 lg TCID50/cm3. Taking into account that modern cell cultivation conditions allow primary trypsinized cell populations subcultivation up to 25–30th passage, subcultures together with continuous cell lines can be used for large-scale sheep and goat poxvirus production and research purposes.https://veterinary.arriah.ru/jour/article/view/413sheep and goat kidney and testicle tissue subculturessheep poxvirusgoat poxviruscytopathic effect |
| spellingShingle | A. B. Sarbasov B. L. Manin R. V. Yashin I. N. Shumilova V. I. Diev TESTING SHEEP AND GOAT POX VIRUSES FOR THEIR REPRODUCTION IN PRIMARY AND SUBCULTURED CELLS Ветеринария сегодня sheep and goat kidney and testicle tissue subcultures sheep poxvirus goat poxvirus cytopathic effect |
| title | TESTING SHEEP AND GOAT POX VIRUSES FOR THEIR REPRODUCTION IN PRIMARY AND SUBCULTURED CELLS |
| title_full | TESTING SHEEP AND GOAT POX VIRUSES FOR THEIR REPRODUCTION IN PRIMARY AND SUBCULTURED CELLS |
| title_fullStr | TESTING SHEEP AND GOAT POX VIRUSES FOR THEIR REPRODUCTION IN PRIMARY AND SUBCULTURED CELLS |
| title_full_unstemmed | TESTING SHEEP AND GOAT POX VIRUSES FOR THEIR REPRODUCTION IN PRIMARY AND SUBCULTURED CELLS |
| title_short | TESTING SHEEP AND GOAT POX VIRUSES FOR THEIR REPRODUCTION IN PRIMARY AND SUBCULTURED CELLS |
| title_sort | testing sheep and goat pox viruses for their reproduction in primary and subcultured cells |
| topic | sheep and goat kidney and testicle tissue subcultures sheep poxvirus goat poxvirus cytopathic effect |
| url | https://veterinary.arriah.ru/jour/article/view/413 |
| work_keys_str_mv | AT absarbasov testingsheepandgoatpoxvirusesfortheirreproductioninprimaryandsubculturedcells AT blmanin testingsheepandgoatpoxvirusesfortheirreproductioninprimaryandsubculturedcells AT rvyashin testingsheepandgoatpoxvirusesfortheirreproductioninprimaryandsubculturedcells AT inshumilova testingsheepandgoatpoxvirusesfortheirreproductioninprimaryandsubculturedcells AT vidiev testingsheepandgoatpoxvirusesfortheirreproductioninprimaryandsubculturedcells |