Leukocyte activation by (1→3)-β-D glucans
We studied the activities of several kinds of β-glucans, including sonifilan, grifolan, Sclerotinia sclerotiorum glucan, laminarin and zymosan, on macrophages. Preculture of macrophages with inactive β-glucans rendered the cells unresponsive to subsequent stimulation with grifolan, suggesting a spec...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1997-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629359791569 |
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| _version_ | 1832561122469740544 |
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| author | Yoshiyuki Adachi Mitsuhiro Okazaki Naohito Ohno Toshiro Yadomae |
| author_facet | Yoshiyuki Adachi Mitsuhiro Okazaki Naohito Ohno Toshiro Yadomae |
| author_sort | Yoshiyuki Adachi |
| collection | DOAJ |
| description | We studied the activities of several kinds of β-glucans, including sonifilan, grifolan, Sclerotinia sclerotiorum glucan, laminarin and zymosan, on macrophages. Preculture of macrophages with inactive β-glucans rendered the cells unresponsive to subsequent stimulation with grifolan, suggesting a specific pathway in the β-glucan structure. The importance of protein C and phosphorylation of mitogen-activated protein kinase was demonstrated in the activation with grifolan or zymosan. Immunoprecipitation of complement receptor (CR3), coprecipitated other proteins carrying phosphotyrosine residues in stimulation with grifolan. These data suggest that protein kinase C and tyrosine kinases are essential for signal transduction, and that CR3 might participate in the activation through interaction with other intracellular proteins. |
| format | Article |
| id | doaj-art-fbd7be98a59d4cf6b20efba8483cc869 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1997-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-fbd7be98a59d4cf6b20efba8483cc8692025-02-03T01:25:59ZengWileyMediators of Inflammation0962-93511466-18611997-01-016425125610.1080/09629359791569Leukocyte activation by (1→3)-β-D glucansYoshiyuki Adachi0Mitsuhiro Okazaki1Naohito Ohno2Toshiro Yadomae3Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, 1432-1 Horinouchi, Hachioji-shi, Tokyo 192-03, JapanLaboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, 1432-1 Horinouchi, Hachioji-shi, Tokyo 192-03, JapanLaboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, 1432-1 Horinouchi, Hachioji-shi, Tokyo 192-03, JapanLaboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, 1432-1 Horinouchi, Hachioji-shi, Tokyo 192-03, JapanWe studied the activities of several kinds of β-glucans, including sonifilan, grifolan, Sclerotinia sclerotiorum glucan, laminarin and zymosan, on macrophages. Preculture of macrophages with inactive β-glucans rendered the cells unresponsive to subsequent stimulation with grifolan, suggesting a specific pathway in the β-glucan structure. The importance of protein C and phosphorylation of mitogen-activated protein kinase was demonstrated in the activation with grifolan or zymosan. Immunoprecipitation of complement receptor (CR3), coprecipitated other proteins carrying phosphotyrosine residues in stimulation with grifolan. These data suggest that protein kinase C and tyrosine kinases are essential for signal transduction, and that CR3 might participate in the activation through interaction with other intracellular proteins.http://dx.doi.org/10.1080/09629359791569 |
| spellingShingle | Yoshiyuki Adachi Mitsuhiro Okazaki Naohito Ohno Toshiro Yadomae Leukocyte activation by (1→3)-β-D glucans Mediators of Inflammation |
| title | Leukocyte activation by (1→3)-β-D glucans |
| title_full | Leukocyte activation by (1→3)-β-D glucans |
| title_fullStr | Leukocyte activation by (1→3)-β-D glucans |
| title_full_unstemmed | Leukocyte activation by (1→3)-β-D glucans |
| title_short | Leukocyte activation by (1→3)-β-D glucans |
| title_sort | leukocyte activation by 1 3 β d glucans |
| url | http://dx.doi.org/10.1080/09629359791569 |
| work_keys_str_mv | AT yoshiyukiadachi leukocyteactivationby13bdglucans AT mitsuhirookazaki leukocyteactivationby13bdglucans AT naohitoohno leukocyteactivationby13bdglucans AT toshiroyadomae leukocyteactivationby13bdglucans |