Bioactive constituents from the leaves of Quercus phillyraeoides A. Gray for α-glucosidase inhibitor activity with concurrent antioxidant activity
Several α-glucosidase inhibitory constituents were isolated from the methanolic extract of the leaves of Quercus phillyraeoides A. Gray (Q. phillyraeoides) using a bioassay-guided fractionation technique. Further separation and purification of the methanol-soluble fraction led to the isolation of co...
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Tsinghua University Press
2016-06-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453016300234 |
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| author | Anastasia Wheni Indrianingsih Sanro Tachibana |
| author_facet | Anastasia Wheni Indrianingsih Sanro Tachibana |
| author_sort | Anastasia Wheni Indrianingsih |
| collection | DOAJ |
| description | Several α-glucosidase inhibitory constituents were isolated from the methanolic extract of the leaves of Quercus phillyraeoides A. Gray (Q. phillyraeoides) using a bioassay-guided fractionation technique. Further separation and purification of the methanol-soluble fraction led to the isolation of constituents with moderate and strong inhibitory activities against α-glucosidase: β-sitosterol-d-glucoside (1) and condensed tannin fractions (2, 3, 4, 5, and 6). Compound 1 and fractions 2–6 had inhibitory concentration (IC50) values against α-glucosidase from Saccharomyces cerevisiae of 118.8, 2.79, 2.78, 3.10, 2.60, and 3.14 μg/mL, respectively, while quercetin as the standard had an IC50 value of 4.80 μg/mL. Furthermore, the significant antioxidant activities were evaluated using several assays, such as the DPPH radical scavenging, hydrogen peroxide radical scavenging, reducing power, and β-carotene-linoleate bleaching assays, and the results suggested that the isolated constituents showed their possible application for treating the hyperglycemia-induced oxidative stress. The results of the present study showed the potential of Q. phillyraeoides as a rich source of natural antidiabetic medicine. |
| format | Article |
| id | doaj-art-fbd0056ba2d44458959c52b0c27c96d9 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2016-06-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-fbd0056ba2d44458959c52b0c27c96d92025-02-03T10:41:38ZengTsinghua University PressFood Science and Human Wellness2213-45302016-06-0152859410.1016/j.fshw.2016.02.004Bioactive constituents from the leaves of Quercus phillyraeoides A. Gray for α-glucosidase inhibitor activity with concurrent antioxidant activityAnastasia Wheni Indrianingsih0Sanro Tachibana1The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, JapanDepartment of Applied Biosciences, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, JapanSeveral α-glucosidase inhibitory constituents were isolated from the methanolic extract of the leaves of Quercus phillyraeoides A. Gray (Q. phillyraeoides) using a bioassay-guided fractionation technique. Further separation and purification of the methanol-soluble fraction led to the isolation of constituents with moderate and strong inhibitory activities against α-glucosidase: β-sitosterol-d-glucoside (1) and condensed tannin fractions (2, 3, 4, 5, and 6). Compound 1 and fractions 2–6 had inhibitory concentration (IC50) values against α-glucosidase from Saccharomyces cerevisiae of 118.8, 2.79, 2.78, 3.10, 2.60, and 3.14 μg/mL, respectively, while quercetin as the standard had an IC50 value of 4.80 μg/mL. Furthermore, the significant antioxidant activities were evaluated using several assays, such as the DPPH radical scavenging, hydrogen peroxide radical scavenging, reducing power, and β-carotene-linoleate bleaching assays, and the results suggested that the isolated constituents showed their possible application for treating the hyperglycemia-induced oxidative stress. The results of the present study showed the potential of Q. phillyraeoides as a rich source of natural antidiabetic medicine.http://www.sciencedirect.com/science/article/pii/S2213453016300234Quercus phillyraeoides A. Grayα-Glucosidase inhibitorAntioxidative activityLineweaver–Burk plot |
| spellingShingle | Anastasia Wheni Indrianingsih Sanro Tachibana Bioactive constituents from the leaves of Quercus phillyraeoides A. Gray for α-glucosidase inhibitor activity with concurrent antioxidant activity Food Science and Human Wellness Quercus phillyraeoides A. Gray α-Glucosidase inhibitor Antioxidative activity Lineweaver–Burk plot |
| title | Bioactive constituents from the leaves of Quercus phillyraeoides A. Gray for α-glucosidase inhibitor activity with concurrent antioxidant activity |
| title_full | Bioactive constituents from the leaves of Quercus phillyraeoides A. Gray for α-glucosidase inhibitor activity with concurrent antioxidant activity |
| title_fullStr | Bioactive constituents from the leaves of Quercus phillyraeoides A. Gray for α-glucosidase inhibitor activity with concurrent antioxidant activity |
| title_full_unstemmed | Bioactive constituents from the leaves of Quercus phillyraeoides A. Gray for α-glucosidase inhibitor activity with concurrent antioxidant activity |
| title_short | Bioactive constituents from the leaves of Quercus phillyraeoides A. Gray for α-glucosidase inhibitor activity with concurrent antioxidant activity |
| title_sort | bioactive constituents from the leaves of quercus phillyraeoides a gray for α glucosidase inhibitor activity with concurrent antioxidant activity |
| topic | Quercus phillyraeoides A. Gray α-Glucosidase inhibitor Antioxidative activity Lineweaver–Burk plot |
| url | http://www.sciencedirect.com/science/article/pii/S2213453016300234 |
| work_keys_str_mv | AT anastasiawheniindrianingsih bioactiveconstituentsfromtheleavesofquercusphillyraeoidesagrayforaglucosidaseinhibitoractivitywithconcurrentantioxidantactivity AT sanrotachibana bioactiveconstituentsfromtheleavesofquercusphillyraeoidesagrayforaglucosidaseinhibitoractivitywithconcurrentantioxidantactivity |