Determination of Arctiin and Arctigenin Contents in Arctium Tomentosum Mill. by HPLC Method
A simple, precise, rapid and accurate, binary-phase high performance liquid chromatographic method has been developed for the determination of arctiin and arctigenin contents in the Arctium tomentosumMill. with short run time. Chromatographic separation was achieved by using HPLC system, consisting...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2011/517681 |
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| author | Xiaoying Zhou Haoke Zhang Liang Ge Haiyan Gong Shuge Tian |
| author_facet | Xiaoying Zhou Haoke Zhang Liang Ge Haiyan Gong Shuge Tian |
| author_sort | Xiaoying Zhou |
| collection | DOAJ |
| description | A simple, precise, rapid and accurate, binary-phase high performance liquid chromatographic method has been developed for the determination of arctiin and arctigenin contents in the Arctium tomentosumMill. with short run time. Chromatographic separation was achieved by using HPLC system, consisting of a Shimadzu LC-6AD and Kromasil C18 column (250×4.6 mm, 5 μm, with pre-column), the mobile phase consists of methanol and water (55: 45). Detection wavelength was 280 nm. The speed of flow was 1.0 mL/min. The specimen handing quantity was 10 μL. The arctiin’s linearity range was 1.575∼4.725 μg (r=0.9995). The arctigenin’s linearity range was 0.613, 3.063 μg (r = 0.9998) and the linear relationship was accurate. The average recovery (n=5) of arctiin and arctigenin were 101.55% (RSD=2.23%) 101.63% (RSD =1.49 %) respectively. The contents of arctiin and arctigenin in Arctium tomentosum Mill. were 10.69 mg/g and 0.15 mg/g, respectively. Therefore， the developed HPLC method can be applied to both in vitro studies of arctiin and arctigenin formulations as well as drug estimation in biological samples. |
| format | Article |
| id | doaj-art-fbc40e4065484cc0a758a42d2c5e758b |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-fbc40e4065484cc0a758a42d2c5e758b2025-02-03T01:10:16ZengWileyE-Journal of Chemistry0973-49452090-98102011-01-018S1S372S37610.1155/2011/517681Determination of Arctiin and Arctigenin Contents in Arctium Tomentosum Mill. by HPLC MethodXiaoying Zhou0Haoke Zhang1Liang Ge2Haiyan Gong3Shuge Tian4Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi-830011, Xinjiang, ChinaXinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi-830011, Xinjiang, ChinaXinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi-830011, Xinjiang, ChinaXinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi-830011, Xinjiang, ChinaXinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi-830011, Xinjiang, ChinaA simple, precise, rapid and accurate, binary-phase high performance liquid chromatographic method has been developed for the determination of arctiin and arctigenin contents in the Arctium tomentosumMill. with short run time. Chromatographic separation was achieved by using HPLC system, consisting of a Shimadzu LC-6AD and Kromasil C18 column (250×4.6 mm, 5 μm, with pre-column), the mobile phase consists of methanol and water (55: 45). Detection wavelength was 280 nm. The speed of flow was 1.0 mL/min. The specimen handing quantity was 10 μL. The arctiin’s linearity range was 1.575∼4.725 μg (r=0.9995). The arctigenin’s linearity range was 0.613, 3.063 μg (r = 0.9998) and the linear relationship was accurate. The average recovery (n=5) of arctiin and arctigenin were 101.55% (RSD=2.23%) 101.63% (RSD =1.49 %) respectively. The contents of arctiin and arctigenin in Arctium tomentosum Mill. were 10.69 mg/g and 0.15 mg/g, respectively. Therefore， the developed HPLC method can be applied to both in vitro studies of arctiin and arctigenin formulations as well as drug estimation in biological samples.http://dx.doi.org/10.1155/2011/517681 |
| spellingShingle | Xiaoying Zhou Haoke Zhang Liang Ge Haiyan Gong Shuge Tian Determination of Arctiin and Arctigenin Contents in Arctium Tomentosum Mill. by HPLC Method E-Journal of Chemistry |
| title | Determination of Arctiin and Arctigenin Contents in Arctium Tomentosum Mill. by HPLC Method |
| title_full | Determination of Arctiin and Arctigenin Contents in Arctium Tomentosum Mill. by HPLC Method |
| title_fullStr | Determination of Arctiin and Arctigenin Contents in Arctium Tomentosum Mill. by HPLC Method |
| title_full_unstemmed | Determination of Arctiin and Arctigenin Contents in Arctium Tomentosum Mill. by HPLC Method |
| title_short | Determination of Arctiin and Arctigenin Contents in Arctium Tomentosum Mill. by HPLC Method |
| title_sort | determination of arctiin and arctigenin contents in arctium tomentosum mill by hplc method |
| url | http://dx.doi.org/10.1155/2011/517681 |
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