Urbanization enhances consumer protist-driven ARGs dissemination in riverine ecosystems
Despite the emergence of antibiotic resistance genes (ARGs) and antibiotic resistant bacteria (ARBs), how biological inter-trophic interactions, modulated by watershed urbanization, shape the resistome remains unexplored. We collected water samples from the highly urbanized (western: 65 % built land...
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Elsevier
2025-01-01
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| Series: | Environment International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412024008250 |
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| author | Bob Adyari Lanping Zhang Ana Maravić Jiaxin Chen Laiyi Li Mahmoud Gad Chang-Ping Yu Anyi Hu |
| author_facet | Bob Adyari Lanping Zhang Ana Maravić Jiaxin Chen Laiyi Li Mahmoud Gad Chang-Ping Yu Anyi Hu |
| author_sort | Bob Adyari |
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| description | Despite the emergence of antibiotic resistance genes (ARGs) and antibiotic resistant bacteria (ARBs), how biological inter-trophic interactions, modulated by watershed urbanization, shape the resistome remains unexplored. We collected water samples from the highly urbanized (western: 65 % built land, sewage-affected) and lesser-urbanized (northern: 25 % built land, drinking water source) downstream tributaries of the Jiulong River in southeast China over dry and wet seasons. We utilized metagenomic and amplicon (16S and 18S rDNA) sequencing to investigate the relationships among microeukaryotic algae, consumer protists, bacterial communities, and the resistome. Metagenomic results showed that ARG-MGE-carrying contigs (mobile ARGs), rather than ARG-carrying contigs (non-mobile ARGs), exhibited more pronounced discrepancies between tributaries. A higher total abundance of ARGs and a greater number of co-shared ARGs between pathogen and non-pathogen bacteria were observed in the more urbanized western tributary. Structural equation modeling revealed that consumer protist-bacteria and algae-bacteria cohesions predominantly influenced the resistome in the western and northern tributaries, respectively. Additionally, consumer protists had more significant associations (511 out of 634) with bacteria carrying mobile ARGs in western tributary, while algae had more significant associations (73 out of 105) in northern tributary. These results highlight the distinct inter-trophic driving factors of the resistome modulated by watershed urbanization. |
| format | Article |
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| institution | Kabale University |
| issn | 0160-4120 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Environment International |
| spelling | doaj-art-fbb2f3cbd8a74f6699c78c88e2b016fc2025-01-24T04:44:10ZengElsevierEnvironment International0160-41202025-01-01195109238Urbanization enhances consumer protist-driven ARGs dissemination in riverine ecosystemsBob Adyari0Lanping Zhang1Ana Maravić2Jiaxin Chen3Laiyi Li4Mahmoud Gad5Chang-Ping Yu6Anyi Hu7CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Department of Environmental Engineering, Universitas Pertamina, Jakarta 12220, IndonesiaCAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaDepartment of Biology, Faculty of Science, University of Split, Ruđera Boškovića 33, 21000 Split, CroatiaCarbon Neutral Innovation Research Center, Fujian Key Laboratory of Marine Carbon Sequestration, Xiamen University, Xiamen, 361005, China; College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, ChinaCAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaWater Pollution Research Department, National Research Centre, Cairo 12622, EgyptCAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, ChinaCAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Carbon Neutral Innovation Research Center, Fujian Key Laboratory of Marine Carbon Sequestration, Xiamen University, Xiamen, 361005, China; Corresponding author at: CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.Despite the emergence of antibiotic resistance genes (ARGs) and antibiotic resistant bacteria (ARBs), how biological inter-trophic interactions, modulated by watershed urbanization, shape the resistome remains unexplored. We collected water samples from the highly urbanized (western: 65 % built land, sewage-affected) and lesser-urbanized (northern: 25 % built land, drinking water source) downstream tributaries of the Jiulong River in southeast China over dry and wet seasons. We utilized metagenomic and amplicon (16S and 18S rDNA) sequencing to investigate the relationships among microeukaryotic algae, consumer protists, bacterial communities, and the resistome. Metagenomic results showed that ARG-MGE-carrying contigs (mobile ARGs), rather than ARG-carrying contigs (non-mobile ARGs), exhibited more pronounced discrepancies between tributaries. A higher total abundance of ARGs and a greater number of co-shared ARGs between pathogen and non-pathogen bacteria were observed in the more urbanized western tributary. Structural equation modeling revealed that consumer protist-bacteria and algae-bacteria cohesions predominantly influenced the resistome in the western and northern tributaries, respectively. Additionally, consumer protists had more significant associations (511 out of 634) with bacteria carrying mobile ARGs in western tributary, while algae had more significant associations (73 out of 105) in northern tributary. These results highlight the distinct inter-trophic driving factors of the resistome modulated by watershed urbanization.http://www.sciencedirect.com/science/article/pii/S0160412024008250UrbanizationARGsARBsInter-trophic interactionsAlgaeConsumer protist |
| spellingShingle | Bob Adyari Lanping Zhang Ana Maravić Jiaxin Chen Laiyi Li Mahmoud Gad Chang-Ping Yu Anyi Hu Urbanization enhances consumer protist-driven ARGs dissemination in riverine ecosystems Environment International Urbanization ARGs ARBs Inter-trophic interactions Algae Consumer protist |
| title | Urbanization enhances consumer protist-driven ARGs dissemination in riverine ecosystems |
| title_full | Urbanization enhances consumer protist-driven ARGs dissemination in riverine ecosystems |
| title_fullStr | Urbanization enhances consumer protist-driven ARGs dissemination in riverine ecosystems |
| title_full_unstemmed | Urbanization enhances consumer protist-driven ARGs dissemination in riverine ecosystems |
| title_short | Urbanization enhances consumer protist-driven ARGs dissemination in riverine ecosystems |
| title_sort | urbanization enhances consumer protist driven args dissemination in riverine ecosystems |
| topic | Urbanization ARGs ARBs Inter-trophic interactions Algae Consumer protist |
| url | http://www.sciencedirect.com/science/article/pii/S0160412024008250 |
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