In Vitro Characterization of Multilamellar Fibers with Uniaxially Oriented Electrospun Type I Collagen Scaffolds
Fabrication of an appropriate scaffold is critical in order to recapitulate the architecture and functionality of the native tissue. In this study, we attempted to create favorable collagen fiber alignment and multilamellar with uniaxially oriented layers, using a disc collector by turning mats 90 d...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Advances in Materials Science and Engineering |
| Online Access: | http://dx.doi.org/10.1155/2020/4084317 |
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| author | Alireza Hooshmand-Ardakani Tahereh Talaei-Khozani Mehdi Sadat-Shojai Soghra Bahmanpour Nehleh Zarei-fard |
| author_facet | Alireza Hooshmand-Ardakani Tahereh Talaei-Khozani Mehdi Sadat-Shojai Soghra Bahmanpour Nehleh Zarei-fard |
| author_sort | Alireza Hooshmand-Ardakani |
| collection | DOAJ |
| description | Fabrication of an appropriate scaffold is critical in order to recapitulate the architecture and functionality of the native tissue. In this study, we attempted to create favorable collagen fiber alignment and multilamellar with uniaxially oriented layers, using a disc collector by turning mats 90 degrees horizontally at specific times. Different concentrations of rat tail-derived type I collagen (3, 6, 8% w/v) in 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) are used for electrospinning affairs. The 6% w/v collagen at an applied voltage of 20 kV and collector rotation of 2500 rpm was selected to exhibit bead-free homogeneous nanofiber with fiber thickness of 0.14 ± 0.4 µm, maximum thickness of 0.5 ± 0.08 µm, and 60% porosity. Also, scanning electron microscope images of electrospun fibers showed 3D multilamellar scaffold with the goodness of 96.5% ± 0.8 in each aligned uniaxially oriented fiber layer. Cross-linking of collagen fibers with N-(3-dimethylaminopropyl)-N0-ethylcarbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS) reduced the fiber degradation rate and preserved the fiber morphology and alignment. The multilamellar mat showed significant increase in tensile strength and average breaking elongation in comparison with unilamellar mat. In vitro cell culture, using human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) on cross-linked scaffold, showed improvement in cell proliferation, attachment, migration, and intercellular junction with a flattened morphology. Raman spectra revealed the preservation of collagen structure. In addition, Raman spectra of the cell containing scaffold were the same as those of an intact intervertebral disc as a sample to be used in engineering tissues. In conclusion, our results showed that the 3D multilamellar collagen nanofibrous scaffold is more appropriate for the tissues that have multilamellar structure. |
| format | Article |
| id | doaj-art-fb854efc6bd04ad7bb79453aadf96249 |
| institution | Kabale University |
| issn | 1687-8434 1687-8442 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Materials Science and Engineering |
| spelling | doaj-art-fb854efc6bd04ad7bb79453aadf962492025-02-03T01:00:19ZengWileyAdvances in Materials Science and Engineering1687-84341687-84422020-01-01202010.1155/2020/40843174084317In Vitro Characterization of Multilamellar Fibers with Uniaxially Oriented Electrospun Type I Collagen ScaffoldsAlireza Hooshmand-Ardakani0Tahereh Talaei-Khozani1Mehdi Sadat-Shojai2Soghra Bahmanpour3Nehleh Zarei-fard4Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranAnatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Chemistry, College of Sciences, Shiraz University, Shiraz, IranAnatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranAnatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranFabrication of an appropriate scaffold is critical in order to recapitulate the architecture and functionality of the native tissue. In this study, we attempted to create favorable collagen fiber alignment and multilamellar with uniaxially oriented layers, using a disc collector by turning mats 90 degrees horizontally at specific times. Different concentrations of rat tail-derived type I collagen (3, 6, 8% w/v) in 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) are used for electrospinning affairs. The 6% w/v collagen at an applied voltage of 20 kV and collector rotation of 2500 rpm was selected to exhibit bead-free homogeneous nanofiber with fiber thickness of 0.14 ± 0.4 µm, maximum thickness of 0.5 ± 0.08 µm, and 60% porosity. Also, scanning electron microscope images of electrospun fibers showed 3D multilamellar scaffold with the goodness of 96.5% ± 0.8 in each aligned uniaxially oriented fiber layer. Cross-linking of collagen fibers with N-(3-dimethylaminopropyl)-N0-ethylcarbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS) reduced the fiber degradation rate and preserved the fiber morphology and alignment. The multilamellar mat showed significant increase in tensile strength and average breaking elongation in comparison with unilamellar mat. In vitro cell culture, using human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) on cross-linked scaffold, showed improvement in cell proliferation, attachment, migration, and intercellular junction with a flattened morphology. Raman spectra revealed the preservation of collagen structure. In addition, Raman spectra of the cell containing scaffold were the same as those of an intact intervertebral disc as a sample to be used in engineering tissues. In conclusion, our results showed that the 3D multilamellar collagen nanofibrous scaffold is more appropriate for the tissues that have multilamellar structure.http://dx.doi.org/10.1155/2020/4084317 |
| spellingShingle | Alireza Hooshmand-Ardakani Tahereh Talaei-Khozani Mehdi Sadat-Shojai Soghra Bahmanpour Nehleh Zarei-fard In Vitro Characterization of Multilamellar Fibers with Uniaxially Oriented Electrospun Type I Collagen Scaffolds Advances in Materials Science and Engineering |
| title | In Vitro Characterization of Multilamellar Fibers with Uniaxially Oriented Electrospun Type I Collagen Scaffolds |
| title_full | In Vitro Characterization of Multilamellar Fibers with Uniaxially Oriented Electrospun Type I Collagen Scaffolds |
| title_fullStr | In Vitro Characterization of Multilamellar Fibers with Uniaxially Oriented Electrospun Type I Collagen Scaffolds |
| title_full_unstemmed | In Vitro Characterization of Multilamellar Fibers with Uniaxially Oriented Electrospun Type I Collagen Scaffolds |
| title_short | In Vitro Characterization of Multilamellar Fibers with Uniaxially Oriented Electrospun Type I Collagen Scaffolds |
| title_sort | in vitro characterization of multilamellar fibers with uniaxially oriented electrospun type i collagen scaffolds |
| url | http://dx.doi.org/10.1155/2020/4084317 |
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