Wolbachia, Spiroplasma, and Rickettsia symbiotic bacteria in aphids (Aphidoidea)
Aphids are a diverse family of crop pests. Aphids formed a complex relationship with intracellular bacteria. Depending on the region of study, the species composition of both aphids and their facultative endosymbionts varies. The aim of the work was to determine the occurrence and genetic diversity...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2020-10-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/2783 |
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| author | D. A. Romanov I. A. Zakharov E. V. Shaikevich |
| author_facet | D. A. Romanov I. A. Zakharov E. V. Shaikevich |
| author_sort | D. A. Romanov |
| collection | DOAJ |
| description | Aphids are a diverse family of crop pests. Aphids formed a complex relationship with intracellular bacteria. Depending on the region of study, the species composition of both aphids and their facultative endosymbionts varies. The aim of the work was to determine the occurrence and genetic diversity of Wolbachia, Spiroplasma and Rickettsia symbionts in aphids collected in 2018–2019 in Moscow. For these purposes, 578 aphids from 32 collection sites were tested by PCR using specific primers. At least 21 species of aphids from 14 genera and four families were identified by barcoding method, of which 11 species were infected with endosymbionts. Rickettsia was found in six species, Wolbachia in two species, Spiroplasma in one species. The presence of Rickettsia in Impatientinum asiaticum, Myzus cerasi, Hyalopterus pruni, Eucallipterus tiliae, Chaitophorus tremulae and Wolbachia in Aphis pomi and C. tremulae has been described for the first time. A double infection with Rickettsia and Spiroplasma was detected in a half of pea aphid (Acyrthosiphon pisum) individuals. For the first time was found that six species of aphids are infected with Rickettsia that are genetically different from previously known. It was first discovered that A. pomi is infected with two Wolbachia strains, one of which belongs to supergroup B and is genetically close to Wolbachia from C. tremulae. The second Wolbachia strain from A. pomi belongs to the supergroup M, recently described in aphid species. Spiroplasma, which we observed in A. pisum, is genetically close to male killing Spiroplasma from aphids, ladybirds and moths. Both maternal inheritance and horizontal transmission are the pathways for the distribution of facultative endosymbiotic bacteria in aphids. |
| format | Article |
| id | doaj-art-fb7c32998754469aa7900bf05d32604b |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-fb7c32998754469aa7900bf05d32604b2025-02-01T09:58:09ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592020-10-0124667368210.18699/VJ20.6611095Wolbachia, Spiroplasma, and Rickettsia symbiotic bacteria in aphids (Aphidoidea)D. A. Romanov0I. A. Zakharov1E. V. Shaikevich2Vavilov Institute of General Genetics of the Russian Academy of Sciences; Moscow Region State UniversityVavilov Institute of General Genetics of the Russian Academy of SciencesVavilov Institute of General Genetics of the Russian Academy of Sciences; Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases, Sechenov First Moscow State Меdical UniversityAphids are a diverse family of crop pests. Aphids formed a complex relationship with intracellular bacteria. Depending on the region of study, the species composition of both aphids and their facultative endosymbionts varies. The aim of the work was to determine the occurrence and genetic diversity of Wolbachia, Spiroplasma and Rickettsia symbionts in aphids collected in 2018–2019 in Moscow. For these purposes, 578 aphids from 32 collection sites were tested by PCR using specific primers. At least 21 species of aphids from 14 genera and four families were identified by barcoding method, of which 11 species were infected with endosymbionts. Rickettsia was found in six species, Wolbachia in two species, Spiroplasma in one species. The presence of Rickettsia in Impatientinum asiaticum, Myzus cerasi, Hyalopterus pruni, Eucallipterus tiliae, Chaitophorus tremulae and Wolbachia in Aphis pomi and C. tremulae has been described for the first time. A double infection with Rickettsia and Spiroplasma was detected in a half of pea aphid (Acyrthosiphon pisum) individuals. For the first time was found that six species of aphids are infected with Rickettsia that are genetically different from previously known. It was first discovered that A. pomi is infected with two Wolbachia strains, one of which belongs to supergroup B and is genetically close to Wolbachia from C. tremulae. The second Wolbachia strain from A. pomi belongs to the supergroup M, recently described in aphid species. Spiroplasma, which we observed in A. pisum, is genetically close to male killing Spiroplasma from aphids, ladybirds and moths. Both maternal inheritance and horizontal transmission are the pathways for the distribution of facultative endosymbiotic bacteria in aphids.https://vavilov.elpub.ru/jour/article/view/2783aphidsendosymbiontspcrplant pestsmutualism |
| spellingShingle | D. A. Romanov I. A. Zakharov E. V. Shaikevich Wolbachia, Spiroplasma, and Rickettsia symbiotic bacteria in aphids (Aphidoidea) Вавиловский журнал генетики и селекции aphids endosymbionts pcr plant pests mutualism |
| title | Wolbachia, Spiroplasma, and Rickettsia symbiotic bacteria in aphids (Aphidoidea) |
| title_full | Wolbachia, Spiroplasma, and Rickettsia symbiotic bacteria in aphids (Aphidoidea) |
| title_fullStr | Wolbachia, Spiroplasma, and Rickettsia symbiotic bacteria in aphids (Aphidoidea) |
| title_full_unstemmed | Wolbachia, Spiroplasma, and Rickettsia symbiotic bacteria in aphids (Aphidoidea) |
| title_short | Wolbachia, Spiroplasma, and Rickettsia symbiotic bacteria in aphids (Aphidoidea) |
| title_sort | wolbachia spiroplasma and rickettsia symbiotic bacteria in aphids aphidoidea |
| topic | aphids endosymbionts pcr plant pests mutualism |
| url | https://vavilov.elpub.ru/jour/article/view/2783 |
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