Influenza A Virus and Influenza B Virus Can Induce Apoptosis via Intrinsic or Extrinsic Pathways and Also via NF-κB in a Time and Dose Dependent Manner
Influenza viruses are able to cause annual epidemics and pandemics due to their mutation rates and reassortment capabilities leading to antigenic shifts and drifts. To identify host response to influenza A and B viruses on A549 and MDCK II cells at low and high MOIs, expressions of MxA and caspases...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2016/1738237 |
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| _version_ | 1832559943591395328 |
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| author | Ibrahim El-Sayed Khalid Bassiouny Aziz Nokaly Ahmed S. Abdelghani Wael Roshdy |
| author_facet | Ibrahim El-Sayed Khalid Bassiouny Aziz Nokaly Ahmed S. Abdelghani Wael Roshdy |
| author_sort | Ibrahim El-Sayed |
| collection | DOAJ |
| description | Influenza viruses are able to cause annual epidemics and pandemics due to their mutation rates and reassortment capabilities leading to antigenic shifts and drifts. To identify host response to influenza A and B viruses on A549 and MDCK II cells at low and high MOIs, expressions of MxA and caspases 3, 8, and 9 and BAD, TNFα, and IκBα genes were measured in the cells supernatants. H1N1 and H3N2 prefer to initially enhance the intrinsic pathway, determined by higher caspase 9 activity in MDCK II cells compared to caspase 8 activity and vice versa in A549 cells at different MOIs, while INF B prefers extrinsic pathway in A549 cells according to significant low or undetectable caspase 9 activity and high activity of caspase 8 but also can induce intrinsic pathway in MDCK II cells as determined by significant low or undetectable activity of caspase 8 and high caspase 9 activity at different MOIs; the considerable MxA expression was found in influenza A and B viruses infected A549 and MDCK II cells at low MOIs. In conclusion, influenza A and B viruses induced extrinsic and intrinsic apoptosis in parallel, and the induction was associated with viral infection in a dose dependent manner. |
| format | Article |
| id | doaj-art-fb6a17d3546049eaa6af6aba6135c052 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-fb6a17d3546049eaa6af6aba6135c0522025-02-03T01:28:58ZengWileyBiochemistry Research International2090-22472090-22552016-01-01201610.1155/2016/17382371738237Influenza A Virus and Influenza B Virus Can Induce Apoptosis via Intrinsic or Extrinsic Pathways and Also via NF-κB in a Time and Dose Dependent MannerIbrahim El-Sayed0Khalid Bassiouny1Aziz Nokaly2Ahmed S. Abdelghani3Wael Roshdy4Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City 32897, EgyptGenetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City 32897, EgyptFaculty of Medicine, Al-Azhar University, Cairo 35527, EgyptCentral Public Health Laboratories (CPHL), Cairo 11613, EgyptCentral Public Health Laboratories (CPHL), Cairo 11613, EgyptInfluenza viruses are able to cause annual epidemics and pandemics due to their mutation rates and reassortment capabilities leading to antigenic shifts and drifts. To identify host response to influenza A and B viruses on A549 and MDCK II cells at low and high MOIs, expressions of MxA and caspases 3, 8, and 9 and BAD, TNFα, and IκBα genes were measured in the cells supernatants. H1N1 and H3N2 prefer to initially enhance the intrinsic pathway, determined by higher caspase 9 activity in MDCK II cells compared to caspase 8 activity and vice versa in A549 cells at different MOIs, while INF B prefers extrinsic pathway in A549 cells according to significant low or undetectable caspase 9 activity and high activity of caspase 8 but also can induce intrinsic pathway in MDCK II cells as determined by significant low or undetectable activity of caspase 8 and high caspase 9 activity at different MOIs; the considerable MxA expression was found in influenza A and B viruses infected A549 and MDCK II cells at low MOIs. In conclusion, influenza A and B viruses induced extrinsic and intrinsic apoptosis in parallel, and the induction was associated with viral infection in a dose dependent manner.http://dx.doi.org/10.1155/2016/1738237 |
| spellingShingle | Ibrahim El-Sayed Khalid Bassiouny Aziz Nokaly Ahmed S. Abdelghani Wael Roshdy Influenza A Virus and Influenza B Virus Can Induce Apoptosis via Intrinsic or Extrinsic Pathways and Also via NF-κB in a Time and Dose Dependent Manner Biochemistry Research International |
| title | Influenza A Virus and Influenza B Virus Can Induce Apoptosis via Intrinsic or Extrinsic Pathways and Also via NF-κB in a Time and Dose Dependent Manner |
| title_full | Influenza A Virus and Influenza B Virus Can Induce Apoptosis via Intrinsic or Extrinsic Pathways and Also via NF-κB in a Time and Dose Dependent Manner |
| title_fullStr | Influenza A Virus and Influenza B Virus Can Induce Apoptosis via Intrinsic or Extrinsic Pathways and Also via NF-κB in a Time and Dose Dependent Manner |
| title_full_unstemmed | Influenza A Virus and Influenza B Virus Can Induce Apoptosis via Intrinsic or Extrinsic Pathways and Also via NF-κB in a Time and Dose Dependent Manner |
| title_short | Influenza A Virus and Influenza B Virus Can Induce Apoptosis via Intrinsic or Extrinsic Pathways and Also via NF-κB in a Time and Dose Dependent Manner |
| title_sort | influenza a virus and influenza b virus can induce apoptosis via intrinsic or extrinsic pathways and also via nf κb in a time and dose dependent manner |
| url | http://dx.doi.org/10.1155/2016/1738237 |
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