Energy status regulated umami compound metabolism in harvested shiitake mushrooms (Lentinus edodes) with spores triggered to release
The molecular mechanisms of energy status related to the umami taste of postharvest shiitake mushrooms during spore release remain poorly understood. In this study, the variations of energy status and umami taste of mushrooms were measured at 25 °C. At 24 h storage, slight spore prints of mushrooms...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001276 |
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| author | Rongrong Xia Xuemei Zhao Guang Xin Libin Sun Heran Xu Zhenshan Hou Yunting Li Yafei Wang |
| author_facet | Rongrong Xia Xuemei Zhao Guang Xin Libin Sun Heran Xu Zhenshan Hou Yunting Li Yafei Wang |
| author_sort | Rongrong Xia |
| collection | DOAJ |
| description | The molecular mechanisms of energy status related to the umami taste of postharvest shiitake mushrooms during spore release remain poorly understood. In this study, the variations of energy status and umami taste of mushrooms were measured at 25 °C. At 24 h storage, slight spore prints of mushrooms were first pictured, respiration peaked. Significant ATP decrease and ADP increase were also observed as the initiation of postharvest senescence (P < 0.05). Meanwhile, the activities of phosphohexose isomerase, succinate dehydrogenase, glucose-6-phosphate dehydrogenase and cytochrome c oxidase and the contents of umami nucleotides and amino acids were maintained at higher levels in mushrooms with spore release. Notably, the equivalent umami concentration (EUC) was strongly correlated with energy levels (R = 0.80). Fifteen related gene expression levels in the energy metabolism pathway were downregulated. LecpdP1 and LeAK were significantly expressed in the conversion of ATP into AMP and played key roles in connecting the energy state and umami level. These results provided valuable insights on the umami taste associated with energy metabolism mechanism during postharvest mushroom spore release. |
| format | Article |
| id | doaj-art-fb4f38b42957448f810a802b799d30af |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-fb4f38b42957448f810a802b799d30af2025-02-03T06:55:37ZengTsinghua University PressFood Science and Human Wellness2213-45302023-01-01121303311Energy status regulated umami compound metabolism in harvested shiitake mushrooms (Lentinus edodes) with spores triggered to releaseRongrong Xia0Xuemei Zhao1Guang Xin2Libin Sun3Heran Xu4Zhenshan Hou5Yunting Li6Yafei Wang7College of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, China; Sichuan national inspection and Testing Co., Ltd., Luzhou 646000, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, China; Corresponding author at: College of Food Science, Shenyang Agricultural University, Shenyang 110866, China.College of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, ChinaThe molecular mechanisms of energy status related to the umami taste of postharvest shiitake mushrooms during spore release remain poorly understood. In this study, the variations of energy status and umami taste of mushrooms were measured at 25 °C. At 24 h storage, slight spore prints of mushrooms were first pictured, respiration peaked. Significant ATP decrease and ADP increase were also observed as the initiation of postharvest senescence (P < 0.05). Meanwhile, the activities of phosphohexose isomerase, succinate dehydrogenase, glucose-6-phosphate dehydrogenase and cytochrome c oxidase and the contents of umami nucleotides and amino acids were maintained at higher levels in mushrooms with spore release. Notably, the equivalent umami concentration (EUC) was strongly correlated with energy levels (R = 0.80). Fifteen related gene expression levels in the energy metabolism pathway were downregulated. LecpdP1 and LeAK were significantly expressed in the conversion of ATP into AMP and played key roles in connecting the energy state and umami level. These results provided valuable insights on the umami taste associated with energy metabolism mechanism during postharvest mushroom spore release.http://www.sciencedirect.com/science/article/pii/S2213453022001276Shiitake mushroomsSpore releaseEnergy metabolismUmami tasteTranscriptome |
| spellingShingle | Rongrong Xia Xuemei Zhao Guang Xin Libin Sun Heran Xu Zhenshan Hou Yunting Li Yafei Wang Energy status regulated umami compound metabolism in harvested shiitake mushrooms (Lentinus edodes) with spores triggered to release Food Science and Human Wellness Shiitake mushrooms Spore release Energy metabolism Umami taste Transcriptome |
| title | Energy status regulated umami compound metabolism in harvested shiitake mushrooms (Lentinus edodes) with spores triggered to release |
| title_full | Energy status regulated umami compound metabolism in harvested shiitake mushrooms (Lentinus edodes) with spores triggered to release |
| title_fullStr | Energy status regulated umami compound metabolism in harvested shiitake mushrooms (Lentinus edodes) with spores triggered to release |
| title_full_unstemmed | Energy status regulated umami compound metabolism in harvested shiitake mushrooms (Lentinus edodes) with spores triggered to release |
| title_short | Energy status regulated umami compound metabolism in harvested shiitake mushrooms (Lentinus edodes) with spores triggered to release |
| title_sort | energy status regulated umami compound metabolism in harvested shiitake mushrooms lentinus edodes with spores triggered to release |
| topic | Shiitake mushrooms Spore release Energy metabolism Umami taste Transcriptome |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001276 |
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