Impact of Soybean Bioactive Peptides on Growth, Lipid Metabolism, Antioxidant Ability, Molecular Responses, and Gut Microbiota of Oriental River Prawn (<i>Macrobrachium nipponense</i>) Fed with a Low-Fishmeal Diet
The substitution of fishmeal with high-level soybean meal in the diet of crustaceans usually induces lipid accumulation and oxidative stress in the hepatopancreas. Therefore, it is essential to alleviate these adverse effects. In the present study, SBPs were used to alleviate the negative effects of...
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2024-12-01
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| author | Chang Yang Bo Liu Liangkun Pan Dong Xia Cunxin Sun Xiaochuan Zheng Peng Chen He Hu Qunlan Zhou |
| author_facet | Chang Yang Bo Liu Liangkun Pan Dong Xia Cunxin Sun Xiaochuan Zheng Peng Chen He Hu Qunlan Zhou |
| author_sort | Chang Yang |
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| description | The substitution of fishmeal with high-level soybean meal in the diet of crustaceans usually induces lipid accumulation and oxidative stress in the hepatopancreas. Therefore, it is essential to alleviate these adverse effects. In the present study, SBPs were used to alleviate the negative effects of a fishmeal decrease on the growth performance, lipid metabolism, antioxidant capacity, and gut microbiota of oriental river prawn (<i>Macrobrachium nipponense</i>) in an 8-week feeding trial. Three isonitrogenic and isolipidic diets were prepared as follows: R (reference diet with 32% fishmeal), CT (control diet with 22% fishmeal), and SBP (22% fishmeal with 1.25 g/kg soybean bioactive peptides). The prawns (initial biomass per tank 17 g) were randomly divided into three groups with four replicates. The results showed that the low-fishmeal diet induced the following: (1) the inhibition of growth performance and survival of prawns; (2) an increase in triglyceride content in the hepatopancreas and hemolymph and downregulation of <i>carnitine palmitoyl transferase 1</i> (<i>cpt1</i>) gene expression; (3) a reduction in antioxidant enzymes’ activities and their genes expression levels and an increase malondialdehyde (MDA) content; and (4) an increase in the abundance of the conditional pathogen <i>Pseudomonas</i> in the gut. SBPs supplementation in the CT diet effectively alleviated most of the above adverse effects. SBPs enhanced inducible nitric oxide synthase (iNOS) activity to synthesize nitric oxide (NO) by activating the <i>imd</i>-<i>relish</i> pathway. Most importantly, SBPs increased the potential probiotic <i>Rikenellaceae_RC9_gut_group</i> abundance and decreased the abundance of the conditional pathogen <i>Pseudomonas</i> in the gut. In conclusion, SBPs supplementation can improve low-fishmeal-diet-induced growth inhibition by regulating the gut microbiota composition to ameliorate lipid deposition and oxidative stress and strengthen immune status in oriental river prawn. |
| format | Article |
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| institution | Kabale University |
| issn | 2079-7737 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Biology |
| spelling | doaj-art-fb4ef54f6fb5496bbd5540de980e50fb2025-01-24T13:23:17ZengMDPI AGBiology2079-77372024-12-011411110.3390/biology14010011Impact of Soybean Bioactive Peptides on Growth, Lipid Metabolism, Antioxidant Ability, Molecular Responses, and Gut Microbiota of Oriental River Prawn (<i>Macrobrachium nipponense</i>) Fed with a Low-Fishmeal DietChang Yang0Bo Liu1Liangkun Pan2Dong Xia3Cunxin Sun4Xiaochuan Zheng5Peng Chen6He Hu7Qunlan Zhou8Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaKey Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, ChinaKey Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaKey Laboratory of Aquatic Animal Nutrition and Health, Freshwater Fisheries Research Center, Chinese Academy of Fishery Science, Wuxi 214081, ChinaJiangsu FIELD Technology Co., Ltd., Huaian 223001, ChinaJiangsu FIELD Technology Co., Ltd., Huaian 223001, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaThe substitution of fishmeal with high-level soybean meal in the diet of crustaceans usually induces lipid accumulation and oxidative stress in the hepatopancreas. Therefore, it is essential to alleviate these adverse effects. In the present study, SBPs were used to alleviate the negative effects of a fishmeal decrease on the growth performance, lipid metabolism, antioxidant capacity, and gut microbiota of oriental river prawn (<i>Macrobrachium nipponense</i>) in an 8-week feeding trial. Three isonitrogenic and isolipidic diets were prepared as follows: R (reference diet with 32% fishmeal), CT (control diet with 22% fishmeal), and SBP (22% fishmeal with 1.25 g/kg soybean bioactive peptides). The prawns (initial biomass per tank 17 g) were randomly divided into three groups with four replicates. The results showed that the low-fishmeal diet induced the following: (1) the inhibition of growth performance and survival of prawns; (2) an increase in triglyceride content in the hepatopancreas and hemolymph and downregulation of <i>carnitine palmitoyl transferase 1</i> (<i>cpt1</i>) gene expression; (3) a reduction in antioxidant enzymes’ activities and their genes expression levels and an increase malondialdehyde (MDA) content; and (4) an increase in the abundance of the conditional pathogen <i>Pseudomonas</i> in the gut. SBPs supplementation in the CT diet effectively alleviated most of the above adverse effects. SBPs enhanced inducible nitric oxide synthase (iNOS) activity to synthesize nitric oxide (NO) by activating the <i>imd</i>-<i>relish</i> pathway. Most importantly, SBPs increased the potential probiotic <i>Rikenellaceae_RC9_gut_group</i> abundance and decreased the abundance of the conditional pathogen <i>Pseudomonas</i> in the gut. In conclusion, SBPs supplementation can improve low-fishmeal-diet-induced growth inhibition by regulating the gut microbiota composition to ameliorate lipid deposition and oxidative stress and strengthen immune status in oriental river prawn.https://www.mdpi.com/2079-7737/14/1/11plant-derived peptidefishmeal replacementbiomass increasemicrobiomegut-hepatopancreas axis |
| spellingShingle | Chang Yang Bo Liu Liangkun Pan Dong Xia Cunxin Sun Xiaochuan Zheng Peng Chen He Hu Qunlan Zhou Impact of Soybean Bioactive Peptides on Growth, Lipid Metabolism, Antioxidant Ability, Molecular Responses, and Gut Microbiota of Oriental River Prawn (<i>Macrobrachium nipponense</i>) Fed with a Low-Fishmeal Diet Biology plant-derived peptide fishmeal replacement biomass increase microbiome gut-hepatopancreas axis |
| title | Impact of Soybean Bioactive Peptides on Growth, Lipid Metabolism, Antioxidant Ability, Molecular Responses, and Gut Microbiota of Oriental River Prawn (<i>Macrobrachium nipponense</i>) Fed with a Low-Fishmeal Diet |
| title_full | Impact of Soybean Bioactive Peptides on Growth, Lipid Metabolism, Antioxidant Ability, Molecular Responses, and Gut Microbiota of Oriental River Prawn (<i>Macrobrachium nipponense</i>) Fed with a Low-Fishmeal Diet |
| title_fullStr | Impact of Soybean Bioactive Peptides on Growth, Lipid Metabolism, Antioxidant Ability, Molecular Responses, and Gut Microbiota of Oriental River Prawn (<i>Macrobrachium nipponense</i>) Fed with a Low-Fishmeal Diet |
| title_full_unstemmed | Impact of Soybean Bioactive Peptides on Growth, Lipid Metabolism, Antioxidant Ability, Molecular Responses, and Gut Microbiota of Oriental River Prawn (<i>Macrobrachium nipponense</i>) Fed with a Low-Fishmeal Diet |
| title_short | Impact of Soybean Bioactive Peptides on Growth, Lipid Metabolism, Antioxidant Ability, Molecular Responses, and Gut Microbiota of Oriental River Prawn (<i>Macrobrachium nipponense</i>) Fed with a Low-Fishmeal Diet |
| title_sort | impact of soybean bioactive peptides on growth lipid metabolism antioxidant ability molecular responses and gut microbiota of oriental river prawn i macrobrachium nipponense i fed with a low fishmeal diet |
| topic | plant-derived peptide fishmeal replacement biomass increase microbiome gut-hepatopancreas axis |
| url | https://www.mdpi.com/2079-7737/14/1/11 |
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