Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis
Abstract Plants are exposed to continual changes in the environment. The daily alternation between light and darkness results in massive recurring changes in the carbon budget, and leads to widespread changes in transcript levels. These diurnal changes are superimposed on slower changes in the envir...
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| Format: | Article |
| Language: | English |
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Springer Nature
2009-10-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.1038/msb.2009.68 |
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| _version_ | 1849225809811734528 |
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| author | Maria Piques Waltraud X Schulze Melanie Höhne Björn Usadel Yves Gibon Johann Rohwer Mark Stitt |
| author_facet | Maria Piques Waltraud X Schulze Melanie Höhne Björn Usadel Yves Gibon Johann Rohwer Mark Stitt |
| author_sort | Maria Piques |
| collection | DOAJ |
| description | Abstract Plants are exposed to continual changes in the environment. The daily alternation between light and darkness results in massive recurring changes in the carbon budget, and leads to widespread changes in transcript levels. These diurnal changes are superimposed on slower changes in the environment. Quantitative molecular information about the numbers of ribosomes, of transcripts for 35 enzymes in central metabolism and their loading into polysomes is used to estimate translation rates in Arabidopsis rosettes, and explore the consequences for important sub‐processes in plant growth. Translation rates for individual enzyme are compared with their abundance in the rosette to predict which enzymes are subject to rapid turnover every day, and which are synthesized at rates that would allow only slow adjustments to sustained changes of the environment, or resemble those needed to support the observed rate of growth. Global translation rates are used to estimate the energy costs of protein synthesis and relate them to the plant carbon budget, in particular the rates of starch degradation and respiration at night. |
| format | Article |
| id | doaj-art-fb30214892f94bd2be511ffbbbdbfa6f |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2009-10-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-fb30214892f94bd2be511ffbbbdbfa6f2025-08-24T11:59:03ZengSpringer NatureMolecular Systems Biology1744-42922009-10-015111710.1038/msb.2009.68Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in ArabidopsisMaria Piques0Waltraud X Schulze1Melanie Höhne2Björn Usadel3Yves Gibon4Johann Rohwer5Mark Stitt6Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1Abstract Plants are exposed to continual changes in the environment. The daily alternation between light and darkness results in massive recurring changes in the carbon budget, and leads to widespread changes in transcript levels. These diurnal changes are superimposed on slower changes in the environment. Quantitative molecular information about the numbers of ribosomes, of transcripts for 35 enzymes in central metabolism and their loading into polysomes is used to estimate translation rates in Arabidopsis rosettes, and explore the consequences for important sub‐processes in plant growth. Translation rates for individual enzyme are compared with their abundance in the rosette to predict which enzymes are subject to rapid turnover every day, and which are synthesized at rates that would allow only slow adjustments to sustained changes of the environment, or resemble those needed to support the observed rate of growth. Global translation rates are used to estimate the energy costs of protein synthesis and relate them to the plant carbon budget, in particular the rates of starch degradation and respiration at night.https://doi.org/10.1038/msb.2009.68Arabidopsispolysomesquantitative RT–PCRribosometranslation |
| spellingShingle | Maria Piques Waltraud X Schulze Melanie Höhne Björn Usadel Yves Gibon Johann Rohwer Mark Stitt Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis Molecular Systems Biology Arabidopsis polysomes quantitative RT–PCR ribosome translation |
| title | Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis |
| title_full | Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis |
| title_fullStr | Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis |
| title_full_unstemmed | Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis |
| title_short | Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis |
| title_sort | ribosome and transcript copy numbers polysome occupancy and enzyme dynamics in arabidopsis |
| topic | Arabidopsis polysomes quantitative RT–PCR ribosome translation |
| url | https://doi.org/10.1038/msb.2009.68 |
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