Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154
Renal carcinoma (RCC) is widely accepted as a malignant tumour of urinary system. Long intergenic non-coding RNA 1939 (LINC01939) is a novel lncRNA which was found to be down-regulated in RCC. Thus, we set out to explore the effect and regulation mechanism of LINC01939 in RCC. LINC01939 and miR-154...
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Taylor & Francis Group
2020-01-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2020.1725024 |
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| author | Rongyuan Zhang Weijie Zhang Baocai Xu Chuan Lv Jianquan Hou Guangbo Zhang |
| author_facet | Rongyuan Zhang Weijie Zhang Baocai Xu Chuan Lv Jianquan Hou Guangbo Zhang |
| author_sort | Rongyuan Zhang |
| collection | DOAJ |
| description | Renal carcinoma (RCC) is widely accepted as a malignant tumour of urinary system. Long intergenic non-coding RNA 1939 (LINC01939) is a novel lncRNA which was found to be down-regulated in RCC. Thus, we set out to explore the effect and regulation mechanism of LINC01939 in RCC. LINC01939 and miR-154 in RCC tissues and cell lines were detected using qRT-PCR assay. To examine cellular viability of ACHN and CAKI-1 cells, cell counting kit-8 (CCK-8) assay was exploited here. Flow cytometric analysis was conducted to examine apoptosis. Cell mobility was valued through wound healing assays. Western blotting was applied for examination of proteins related to proliferation, apoptosis, migration and Wnt/β-catenin/Notch. LINC01939 was down-regulated in RCC tissues. LINC01939 overexpression impeded proliferation and migration, and induced apoptosis. Further study found that the overexpression of LINC01939 strongly suppressed miR-154 expression. Then, the inhibiting effect of overexpressed LINC01939 on proliferation and mobility and the promoting role of LINC01939 in apoptosis were abolished by the combination of miR-154 mimic. Finally, we found that overexpressed LINC01939 inactivated Wnt/β-catenin and Notch through suppressing miR-154. Up-regulation of LINC01939 inhibited proliferation and migration of RCC cells by down-regulating miR-154. |
| format | Article |
| id | doaj-art-fb2932ee2c9646a4a7e1d2a19b95cede |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-fb2932ee2c9646a4a7e1d2a19b95cede2025-08-20T04:02:41ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2020-01-0148169570210.1080/21691401.2020.1725024Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154Rongyuan Zhang0Weijie Zhang1Baocai Xu2Chuan Lv3Jianquan Hou4Guangbo Zhang5Department of Urology, The First Affiliated Hospital of Soochow University, Jiangsu, ChinaDepartment of Urology, The First Affiliated Hospital of Soochow University, Jiangsu, ChinaDepartment of Urology, Jining No, 1 People’s Hospital, Jining, ChinaDepartment of Urology, Jining No, 1 People’s Hospital, Jining, ChinaDepartment of Urology, The First Affiliated Hospital of Soochow University, Jiangsu, ChinaJiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Jiangsu, ChinaRenal carcinoma (RCC) is widely accepted as a malignant tumour of urinary system. Long intergenic non-coding RNA 1939 (LINC01939) is a novel lncRNA which was found to be down-regulated in RCC. Thus, we set out to explore the effect and regulation mechanism of LINC01939 in RCC. LINC01939 and miR-154 in RCC tissues and cell lines were detected using qRT-PCR assay. To examine cellular viability of ACHN and CAKI-1 cells, cell counting kit-8 (CCK-8) assay was exploited here. Flow cytometric analysis was conducted to examine apoptosis. Cell mobility was valued through wound healing assays. Western blotting was applied for examination of proteins related to proliferation, apoptosis, migration and Wnt/β-catenin/Notch. LINC01939 was down-regulated in RCC tissues. LINC01939 overexpression impeded proliferation and migration, and induced apoptosis. Further study found that the overexpression of LINC01939 strongly suppressed miR-154 expression. Then, the inhibiting effect of overexpressed LINC01939 on proliferation and mobility and the promoting role of LINC01939 in apoptosis were abolished by the combination of miR-154 mimic. Finally, we found that overexpressed LINC01939 inactivated Wnt/β-catenin and Notch through suppressing miR-154. Up-regulation of LINC01939 inhibited proliferation and migration of RCC cells by down-regulating miR-154.https://www.tandfonline.com/doi/10.1080/21691401.2020.1725024Renal carcinomaLINC01939miR-154proliferationmigration |
| spellingShingle | Rongyuan Zhang Weijie Zhang Baocai Xu Chuan Lv Jianquan Hou Guangbo Zhang Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154 Artificial Cells, Nanomedicine, and Biotechnology Renal carcinoma LINC01939 miR-154 proliferation migration |
| title | Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154 |
| title_full | Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154 |
| title_fullStr | Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154 |
| title_full_unstemmed | Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154 |
| title_short | Long intergenic non-coding RNA 1939 eliminates proliferation and migration of human renal cell carcinoma (RCC) cells by down-regulation of miR-154 |
| title_sort | long intergenic non coding rna 1939 eliminates proliferation and migration of human renal cell carcinoma rcc cells by down regulation of mir 154 |
| topic | Renal carcinoma LINC01939 miR-154 proliferation migration |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2020.1725024 |
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