Magnetic Targeted Delivery of Induced Pluripotent Stem Cells Promotes Articular Cartilage Repair
Cartilage regeneration treatments using stem cells are associated with problems due to the cell source and the difficulty of delivering the cells to the cartilage defect. We consider labeled induced pluripotent stem (iPS) cells to be an ideal source of cells for tissue regeneration, and if iPS cells...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/9514719 |
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| author | Shinji Kotaka Shigeyuki Wakitani Akira Shimamoto Naosuke Kamei Mikiya Sawa Nobuo Adachi Mituo Ochi |
| author_facet | Shinji Kotaka Shigeyuki Wakitani Akira Shimamoto Naosuke Kamei Mikiya Sawa Nobuo Adachi Mituo Ochi |
| author_sort | Shinji Kotaka |
| collection | DOAJ |
| description | Cartilage regeneration treatments using stem cells are associated with problems due to the cell source and the difficulty of delivering the cells to the cartilage defect. We consider labeled induced pluripotent stem (iPS) cells to be an ideal source of cells for tissue regeneration, and if iPS cells could be delivered only into cartilage defects, it would be possible to repair articular cartilage. Consequently, we investigated the effect of magnetically labeled iPS (m-iPS) cells delivered into an osteochondral defect by magnetic field on the repair of articular cartilage. iPS cells were labeled magnetically and assessed for maintenance of pluripotency by their ability to form embryoid bodies in vitro and to form teratomas when injected subcutaneously into nude rats. These cells were delivered specifically into cartilage defects in nude rats using a magnetic field. The samples were graded according to the histologic grading score for cartilage regeneration. m-iPS cells differentiated into three embryonic germ layers and formed teratomas in the subcutaneous tissue. The histologic grading score was significantly better in the treatment group compared to the control group. m-iPS cells maintained pluripotency, and the magnetic delivery system proved useful and safe for cartilage repair using iPS cells. |
| format | Article |
| id | doaj-art-fb208934065c4497acd1e023fc2b42cb |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-fb208934065c4497acd1e023fc2b42cb2025-02-03T07:24:54ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/95147199514719Magnetic Targeted Delivery of Induced Pluripotent Stem Cells Promotes Articular Cartilage RepairShinji Kotaka0Shigeyuki Wakitani1Akira Shimamoto2Naosuke Kamei3Mikiya Sawa4Nobuo Adachi5Mituo Ochi6Department of Orthopaedic Surgery, Division of Medicine, Biomedical Sciences Major, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3 Minami-ku, Hiroshima 734-8551, JapanDepartment of Orthopaedic Surgery, Division of Medicine, Biomedical Sciences Major, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3 Minami-ku, Hiroshima 734-8551, JapanDepartment of Cellular and Molecular Biology, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima 734-8551, JapanDepartment of Orthopaedic Surgery, Division of Medicine, Biomedical Sciences Major, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3 Minami-ku, Hiroshima 734-8551, JapanDepartment of Orthopaedic Surgery, Division of Medicine, Biomedical Sciences Major, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3 Minami-ku, Hiroshima 734-8551, JapanDepartment of Orthopaedic Surgery, Division of Medicine, Biomedical Sciences Major, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3 Minami-ku, Hiroshima 734-8551, JapanDepartment of Orthopaedic Surgery, Division of Medicine, Biomedical Sciences Major, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3 Minami-ku, Hiroshima 734-8551, JapanCartilage regeneration treatments using stem cells are associated with problems due to the cell source and the difficulty of delivering the cells to the cartilage defect. We consider labeled induced pluripotent stem (iPS) cells to be an ideal source of cells for tissue regeneration, and if iPS cells could be delivered only into cartilage defects, it would be possible to repair articular cartilage. Consequently, we investigated the effect of magnetically labeled iPS (m-iPS) cells delivered into an osteochondral defect by magnetic field on the repair of articular cartilage. iPS cells were labeled magnetically and assessed for maintenance of pluripotency by their ability to form embryoid bodies in vitro and to form teratomas when injected subcutaneously into nude rats. These cells were delivered specifically into cartilage defects in nude rats using a magnetic field. The samples were graded according to the histologic grading score for cartilage regeneration. m-iPS cells differentiated into three embryonic germ layers and formed teratomas in the subcutaneous tissue. The histologic grading score was significantly better in the treatment group compared to the control group. m-iPS cells maintained pluripotency, and the magnetic delivery system proved useful and safe for cartilage repair using iPS cells.http://dx.doi.org/10.1155/2017/9514719 |
| spellingShingle | Shinji Kotaka Shigeyuki Wakitani Akira Shimamoto Naosuke Kamei Mikiya Sawa Nobuo Adachi Mituo Ochi Magnetic Targeted Delivery of Induced Pluripotent Stem Cells Promotes Articular Cartilage Repair Stem Cells International |
| title | Magnetic Targeted Delivery of Induced Pluripotent Stem Cells Promotes Articular Cartilage Repair |
| title_full | Magnetic Targeted Delivery of Induced Pluripotent Stem Cells Promotes Articular Cartilage Repair |
| title_fullStr | Magnetic Targeted Delivery of Induced Pluripotent Stem Cells Promotes Articular Cartilage Repair |
| title_full_unstemmed | Magnetic Targeted Delivery of Induced Pluripotent Stem Cells Promotes Articular Cartilage Repair |
| title_short | Magnetic Targeted Delivery of Induced Pluripotent Stem Cells Promotes Articular Cartilage Repair |
| title_sort | magnetic targeted delivery of induced pluripotent stem cells promotes articular cartilage repair |
| url | http://dx.doi.org/10.1155/2017/9514719 |
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