Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia
Introduction: Tuberculosis (TB) continues to be a significant public health problem. The role of small non-coding RNAs, such as microRNAs (miRNAs), was investigated extensively in Mycobacterium tuberculosis (MTB) infection as well as in a variety of other pathophysiological processes in recent year...
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The Journal of Infection in Developing Countries
2022-08-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/16721 |
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| author | Ivana Buha Vesna Škodrić-Trifunović Marina Anđelković Sonja Pavlović Miša Vreća Mihailo Stjepanović Jelena Milin-Lazović Marko Simić Gordana Antonijević Vesna Spasovski |
| author_facet | Ivana Buha Vesna Škodrić-Trifunović Marina Anđelković Sonja Pavlović Miša Vreća Mihailo Stjepanović Jelena Milin-Lazović Marko Simić Gordana Antonijević Vesna Spasovski |
| author_sort | Ivana Buha |
| collection | DOAJ |
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Introduction: Tuberculosis (TB) continues to be a significant public health problem. The role of small non-coding RNAs, such as microRNAs (miRNAs), was investigated extensively in Mycobacterium tuberculosis (MTB) infection as well as in a variety of other pathophysiological processes in recent years. It was found that miRNAs act as regulators of both early reaction to MTB infection and in process of adaptation of the host immune cells during latent course of the disease. Molecule miRNA-146a is expressed exclusively in immune cells and it has the most prominent role in modulation of innate immunity.
Methodology: We investigated the level of expression of miRNA-146a using an RT-qPCR technique in peripheral blood mononuclear cells of 44 patients with active pulmonary TB and 17 healthy individuals. We also analyzed the significance of miRNA-146a rs2910164 SNV for expression profile of miRNA-146a, in order to investigate potential usage of miRNA-146a as a biomarker for TB.
Results: There was statistically significant decrease of expression of miRNA-146a in TB group compared to control group. When gender cohorts were analyzed, the expression levels in TB male and TB female subgroup were significantly lower than the expression levels in the same gender control subgroups.
Conclusions: Our results indicate that miRNA-146a plays a significant role in the pathogenesis of TB, suggesting that miRNA-146a could be used as a biomarker for active pulmonary TB.
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| format | Article |
| id | doaj-art-fae02f2f7b8b4b4fa9bc99e3e8e0fda6 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2022-08-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-fae02f2f7b8b4b4fa9bc99e3e8e0fda62025-08-20T03:52:38ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802022-08-01160810.3855/jidc.16721Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from SerbiaIvana Buha0Vesna Škodrić-Trifunović1Marina Anđelković2Sonja Pavlović3Miša Vreća4Mihailo Stjepanović5Jelena Milin-Lazović6Marko Simić7Gordana Antonijević8Vesna Spasovski9Clinic for Pulmonology, Clinical Centre of Serbia, Belgrade, SerbiaClinic for Pulmonology, Clinical Centre of Serbia, Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, SerbiaClinic for Pulmonology, Clinical Centre of Serbia, Belgrade, SerbiaDepartment for Medical Statistics and Informatics, Faculty of Medicine, University of Belgrade, Belgrade, SerbiaClinic for Orthopedic Surgery and Traumatology, Clinical Center of Serbia, Belgrade, SerbiaSpecial Hospital for Lung Diseases Ozren, Soko banja, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia Introduction: Tuberculosis (TB) continues to be a significant public health problem. The role of small non-coding RNAs, such as microRNAs (miRNAs), was investigated extensively in Mycobacterium tuberculosis (MTB) infection as well as in a variety of other pathophysiological processes in recent years. It was found that miRNAs act as regulators of both early reaction to MTB infection and in process of adaptation of the host immune cells during latent course of the disease. Molecule miRNA-146a is expressed exclusively in immune cells and it has the most prominent role in modulation of innate immunity. Methodology: We investigated the level of expression of miRNA-146a using an RT-qPCR technique in peripheral blood mononuclear cells of 44 patients with active pulmonary TB and 17 healthy individuals. We also analyzed the significance of miRNA-146a rs2910164 SNV for expression profile of miRNA-146a, in order to investigate potential usage of miRNA-146a as a biomarker for TB. Results: There was statistically significant decrease of expression of miRNA-146a in TB group compared to control group. When gender cohorts were analyzed, the expression levels in TB male and TB female subgroup were significantly lower than the expression levels in the same gender control subgroups. Conclusions: Our results indicate that miRNA-146a plays a significant role in the pathogenesis of TB, suggesting that miRNA-146a could be used as a biomarker for active pulmonary TB. https://jidc.org/index.php/journal/article/view/16721miRNA-146a gene expressionbiomarkeractive pulmonary tuberculosis |
| spellingShingle | Ivana Buha Vesna Škodrić-Trifunović Marina Anđelković Sonja Pavlović Miša Vreća Mihailo Stjepanović Jelena Milin-Lazović Marko Simić Gordana Antonijević Vesna Spasovski Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia Journal of Infection in Developing Countries miRNA-146a gene expression biomarker active pulmonary tuberculosis |
| title | Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia |
| title_full | Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia |
| title_fullStr | Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia |
| title_full_unstemmed | Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia |
| title_short | Association between active pulmonary tuberculosis and miRNA-146a: A preliminary study from Serbia |
| title_sort | association between active pulmonary tuberculosis and mirna 146a a preliminary study from serbia |
| topic | miRNA-146a gene expression biomarker active pulmonary tuberculosis |
| url | https://jidc.org/index.php/journal/article/view/16721 |
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