Acute Toxicity and Determination of the Active Constituents of Aqueous Extract of Uncaria tomentosa Bark in Hyphessobrycon eques
Uncaria tomentosa is a medicinal plant used in folk medicine by Amazon tribes. In this study the constituents of aqueous extract of U. tomentosa bark were quantified by chromatographic technique and its lethal concentration 50 (48 h) in Hyphessobrycon eques was determined. The chromatography showed...
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Wiley
2014-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2014/412437 |
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| author | Jefferson Yunis Aguinaga Gustavo S. Claudiano Paulo F. Marcusso Cynthia Ikefuti George G. Ortega Silas F. Eto Claudinei da Cruz Juliet R. E. Moraes Flávio R. Moraes João B. K. Fernandes |
| author_facet | Jefferson Yunis Aguinaga Gustavo S. Claudiano Paulo F. Marcusso Cynthia Ikefuti George G. Ortega Silas F. Eto Claudinei da Cruz Juliet R. E. Moraes Flávio R. Moraes João B. K. Fernandes |
| author_sort | Jefferson Yunis Aguinaga |
| collection | DOAJ |
| description | Uncaria tomentosa is a medicinal plant used in folk medicine by Amazon tribes. In this study the constituents of aqueous extract of U. tomentosa bark were quantified by chromatographic technique and its lethal concentration 50 (48 h) in Hyphessobrycon eques was determined. The chromatography showed high levels of oxindole alkaloids, quinovic acid glycosides, and low molecular weight polyphenols. The CL50 48 h was 1816 mg/L. Fish showed behavior changes at concentrations above 2000 mg/L, accompanied by a significant decrease of dissolved oxygen. At the highest concentration 100% mortality was observed attributed to oxygen reduction by the amount of oxindole alkaloids, polyphenols accumulation of the extract in the gills, and the interaction of these compounds with dopamine. In conclusion, the aqueous extract of U. tomentosa did not alter the chemical components and it was shown that U. tomentosa has low toxicity to H. eques; therefore, it can be used safely in this species. |
| format | Article |
| id | doaj-art-faaa8f2a2f384a72a695105b5b710d3b |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-faaa8f2a2f384a72a695105b5b710d3b2025-02-03T06:01:18ZengWileyJournal of Toxicology1687-81911687-82052014-01-01201410.1155/2014/412437412437Acute Toxicity and Determination of the Active Constituents of Aqueous Extract of Uncaria tomentosa Bark in Hyphessobrycon equesJefferson Yunis Aguinaga0Gustavo S. Claudiano1Paulo F. Marcusso2Cynthia Ikefuti3George G. Ortega4Silas F. Eto5Claudinei da Cruz6Juliet R. E. Moraes7Flávio R. Moraes8João B. K. Fernandes9Aquaculture Center of UNESP, Jaboticabal, SP, BrazilDepartment of Veterinary Pathology, School of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), 14884-900 Jaboticabal, SP, BrazilDepartment of Veterinary Pathology, School of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), 14884-900 Jaboticabal, SP, BrazilWeed Science Environmental Research Studies Centre of UNESP, University Estadual Paulista, Jaboticabal, SP, BrazilFaculty of Pharmacy, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, BrazilDepartment of Veterinary Pathology, School of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), 14884-900 Jaboticabal, SP, BrazilWeed Science Environmental Research Studies Centre of UNESP, University Estadual Paulista, Jaboticabal, SP, BrazilAquaculture Center of UNESP, Jaboticabal, SP, BrazilDepartment of Veterinary Pathology, School of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), 14884-900 Jaboticabal, SP, BrazilAquaculture Center of UNESP, Jaboticabal, SP, BrazilUncaria tomentosa is a medicinal plant used in folk medicine by Amazon tribes. In this study the constituents of aqueous extract of U. tomentosa bark were quantified by chromatographic technique and its lethal concentration 50 (48 h) in Hyphessobrycon eques was determined. The chromatography showed high levels of oxindole alkaloids, quinovic acid glycosides, and low molecular weight polyphenols. The CL50 48 h was 1816 mg/L. Fish showed behavior changes at concentrations above 2000 mg/L, accompanied by a significant decrease of dissolved oxygen. At the highest concentration 100% mortality was observed attributed to oxygen reduction by the amount of oxindole alkaloids, polyphenols accumulation of the extract in the gills, and the interaction of these compounds with dopamine. In conclusion, the aqueous extract of U. tomentosa did not alter the chemical components and it was shown that U. tomentosa has low toxicity to H. eques; therefore, it can be used safely in this species.http://dx.doi.org/10.1155/2014/412437 |
| spellingShingle | Jefferson Yunis Aguinaga Gustavo S. Claudiano Paulo F. Marcusso Cynthia Ikefuti George G. Ortega Silas F. Eto Claudinei da Cruz Juliet R. E. Moraes Flávio R. Moraes João B. K. Fernandes Acute Toxicity and Determination of the Active Constituents of Aqueous Extract of Uncaria tomentosa Bark in Hyphessobrycon eques Journal of Toxicology |
| title | Acute Toxicity and Determination of the Active Constituents of Aqueous Extract of Uncaria tomentosa Bark in Hyphessobrycon eques |
| title_full | Acute Toxicity and Determination of the Active Constituents of Aqueous Extract of Uncaria tomentosa Bark in Hyphessobrycon eques |
| title_fullStr | Acute Toxicity and Determination of the Active Constituents of Aqueous Extract of Uncaria tomentosa Bark in Hyphessobrycon eques |
| title_full_unstemmed | Acute Toxicity and Determination of the Active Constituents of Aqueous Extract of Uncaria tomentosa Bark in Hyphessobrycon eques |
| title_short | Acute Toxicity and Determination of the Active Constituents of Aqueous Extract of Uncaria tomentosa Bark in Hyphessobrycon eques |
| title_sort | acute toxicity and determination of the active constituents of aqueous extract of uncaria tomentosa bark in hyphessobrycon eques |
| url | http://dx.doi.org/10.1155/2014/412437 |
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