The Impact of PTPRK and ROS1 Polymorphisms on the Preeclampsia Risk in Han Chinese Women
Objective. Preeclampsia (PE) is a severe complication in pregnancy and a leading cause of maternal and infant mortality. However, the exact underlying etiology of PE remains unknown. Emerging evidence indicates that the cause of PE is associated with genetic factors. Therefore, the aim of this study...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | International Journal of Hypertension |
| Online Access: | http://dx.doi.org/10.1155/2021/3275081 |
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| author | Huihui Li Xingyu Yan Man Yang Mei Liu Shan Tian Mengru Yu Wei-Ping Li Cong Zhang |
| author_facet | Huihui Li Xingyu Yan Man Yang Mei Liu Shan Tian Mengru Yu Wei-Ping Li Cong Zhang |
| author_sort | Huihui Li |
| collection | DOAJ |
| description | Objective. Preeclampsia (PE) is a severe complication in pregnancy and a leading cause of maternal and infant mortality. However, the exact underlying etiology of PE remains unknown. Emerging evidence indicates that the cause of PE is associated with genetic factors. Therefore, the aim of this study is to identify susceptibility genes to PE. Materials and Methods. Human Exome BeadChip assays were conducted using 370 cases and 482 controls and 21 loci were discovered. A further independent set of 958 cases and 1007 controls were recruited for genotyping to determine whether the genes of interest ROS1 and PTPRK are associated with PE. Immunohistochemistry was used for localization. Both qPCR and Western blotting were utilized to investigate the levels of PTPRK in placentas of 20 PE and 20 normal pregnancies. Results. The allele frequency of PTPRK rs3190930 differed significantly between PE and controls and was particularly significant in severe PE subgroup and early-onset PE subgroup. PTPRK is primarily localized in placental trophoblast cells. The mRNA and protein levels of PTPRK in PE were significantly higher than those in controls. Conclusion. These results suggest that PTPRK appears to be a previously unrecognized susceptibility gene for PE in Han Chinese women, and its expression is also associated with PE, while ROS1 rs9489124 has no apparent correlation with PE risk. |
| format | Article |
| id | doaj-art-fa9e713f26b64857b568d5d9b4e7025e |
| institution | Kabale University |
| issn | 2090-0384 2090-0392 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Hypertension |
| spelling | doaj-art-fa9e713f26b64857b568d5d9b4e7025e2025-02-03T07:24:04ZengWileyInternational Journal of Hypertension2090-03842090-03922021-01-01202110.1155/2021/32750813275081The Impact of PTPRK and ROS1 Polymorphisms on the Preeclampsia Risk in Han Chinese WomenHuihui Li0Xingyu Yan1Man Yang2Mei Liu3Shan Tian4Mengru Yu5Wei-Ping Li6Cong Zhang7Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200135, ChinaFujian Provincial Key Laboratory of Reproductive Health Research, Medical College of Xiamen University, Xiamen, Fujian 361102, ChinaShandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences, Shandong Normal University, 88 East Wenhua Road, Jinan, Shandong 250014, ChinaDepartment of Obstetrics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250011, ChinaCenter for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Shandong Provincial Key Laboratory of Reproductive Medicine, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, The Key Laboratory for Reproductive Endocrinology of Ministry of Education, Jinan 250021, ChinaCenter for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Shandong Provincial Key Laboratory of Reproductive Medicine, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, The Key Laboratory for Reproductive Endocrinology of Ministry of Education, Jinan 250021, ChinaCenter for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200135, ChinaCenter for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200135, ChinaObjective. Preeclampsia (PE) is a severe complication in pregnancy and a leading cause of maternal and infant mortality. However, the exact underlying etiology of PE remains unknown. Emerging evidence indicates that the cause of PE is associated with genetic factors. Therefore, the aim of this study is to identify susceptibility genes to PE. Materials and Methods. Human Exome BeadChip assays were conducted using 370 cases and 482 controls and 21 loci were discovered. A further independent set of 958 cases and 1007 controls were recruited for genotyping to determine whether the genes of interest ROS1 and PTPRK are associated with PE. Immunohistochemistry was used for localization. Both qPCR and Western blotting were utilized to investigate the levels of PTPRK in placentas of 20 PE and 20 normal pregnancies. Results. The allele frequency of PTPRK rs3190930 differed significantly between PE and controls and was particularly significant in severe PE subgroup and early-onset PE subgroup. PTPRK is primarily localized in placental trophoblast cells. The mRNA and protein levels of PTPRK in PE were significantly higher than those in controls. Conclusion. These results suggest that PTPRK appears to be a previously unrecognized susceptibility gene for PE in Han Chinese women, and its expression is also associated with PE, while ROS1 rs9489124 has no apparent correlation with PE risk.http://dx.doi.org/10.1155/2021/3275081 |
| spellingShingle | Huihui Li Xingyu Yan Man Yang Mei Liu Shan Tian Mengru Yu Wei-Ping Li Cong Zhang The Impact of PTPRK and ROS1 Polymorphisms on the Preeclampsia Risk in Han Chinese Women International Journal of Hypertension |
| title | The Impact of PTPRK and ROS1 Polymorphisms on the Preeclampsia Risk in Han Chinese Women |
| title_full | The Impact of PTPRK and ROS1 Polymorphisms on the Preeclampsia Risk in Han Chinese Women |
| title_fullStr | The Impact of PTPRK and ROS1 Polymorphisms on the Preeclampsia Risk in Han Chinese Women |
| title_full_unstemmed | The Impact of PTPRK and ROS1 Polymorphisms on the Preeclampsia Risk in Han Chinese Women |
| title_short | The Impact of PTPRK and ROS1 Polymorphisms on the Preeclampsia Risk in Han Chinese Women |
| title_sort | impact of ptprk and ros1 polymorphisms on the preeclampsia risk in han chinese women |
| url | http://dx.doi.org/10.1155/2021/3275081 |
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