Recombinant Enolase of Trypanosoma cruzi as a Novel Vaccine Candidate against Chagas Disease in a Mouse Model of Acute Infection
Trypanosoma cruzi is the protozoan parasite that causes Chagas disease, which is considered by the World Health Organization to be a neglected tropical disease. Two drugs exist for the treatment of Chagas disease, nifurtimox and benznidazole; they are only effective in the acute phase, and a vaccine...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/8964085 |
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| author | Minerva Arce-Fonseca María Cristina González-Vázquez Olivia Rodríguez-Morales Verónica Graullera-Rivera Alberto Aranda-Fraustro Pedro A. Reyes Alejandro Carabarin-Lima José Luis Rosales-Encina |
| author_facet | Minerva Arce-Fonseca María Cristina González-Vázquez Olivia Rodríguez-Morales Verónica Graullera-Rivera Alberto Aranda-Fraustro Pedro A. Reyes Alejandro Carabarin-Lima José Luis Rosales-Encina |
| author_sort | Minerva Arce-Fonseca |
| collection | DOAJ |
| description | Trypanosoma cruzi is the protozoan parasite that causes Chagas disease, which is considered by the World Health Organization to be a neglected tropical disease. Two drugs exist for the treatment of Chagas disease, nifurtimox and benznidazole; they are only effective in the acute phase, and a vaccine is currently not available. In this study, we used the recombinant enolase from T. cruzi H8 strain (MHOM/MX/1992/H8 Yucatán) (rTcENO) and its encoding DNA (pBKTcENO) to immunize mice and evaluate their protective effects in an experimental murine model of acute phase infection. Our results showed that mice vaccinated with rTcENO or its encoding DNA were able to generate typical specific antibodies (IgG1, IgG2a, and IgG2b), suggesting that a mixed Th1/Th2 immune response was induced. The parasite burden in the blood was reduced to 69.8% and 71% in mice vaccinated with rTcENO and pBKTcENO, respectively. The group vaccinated with rTcENO achieved 75% survival, in contrast to the group vaccinated with pBKTcENO that showed no survival in comparison to the control groups. Moreover, rTcENO immunization elevated the production of IFN-γ and IL-2 after the parasite challenge, suggesting that the Th1-type immune response was polarized. These results indicated that rTcENO could be used as a vaccine against Chagas disease. |
| format | Article |
| id | doaj-art-fa9c23407439403c983986252b30dd69 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-fa9c23407439403c983986252b30dd692025-02-03T06:05:23ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/89640858964085Recombinant Enolase of Trypanosoma cruzi as a Novel Vaccine Candidate against Chagas Disease in a Mouse Model of Acute InfectionMinerva Arce-Fonseca0María Cristina González-Vázquez1Olivia Rodríguez-Morales2Verónica Graullera-Rivera3Alberto Aranda-Fraustro4Pedro A. Reyes5Alejandro Carabarin-Lima6José Luis Rosales-Encina7Departamento de Biología Molecular, Juan Badiano No. 1, Col. Sección XVI, Delegación Tlalpan, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Mexico City, MexicoDepartamento de Biología Celular, Avenida Instituto Politecnico Nacional No. 2508, Col. San Pedro Zacatenco, Centro de Investigación y de Estudios Avanzados del Instituto Politecnico Nacional, 07360 Mexico City, MexicoDepartamento de Biología Molecular, Juan Badiano No. 1, Col. Sección XVI, Delegación Tlalpan, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Mexico City, MexicoDepartamento de Anatomía Patológica, Juan Badiano No. 1, Col. Sección XVI, Delegación Tlalpan, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Mexico City, MexicoDepartamento de Anatomía Patológica, Juan Badiano No. 1, Col. Sección XVI, Delegación Tlalpan, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Mexico City, MexicoDepartamento de Biología Molecular, Juan Badiano No. 1, Col. Sección XVI, Delegación Tlalpan, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Mexico City, MexicoDepartamento de Biología Molecular, Juan Badiano No. 1, Col. Sección XVI, Delegación Tlalpan, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Mexico City, MexicoDepartamento de Infectómica y Patogénesis Molecular, Avenida Instituto Politecnico Nacional No. 2508, Col. San Pedro Zacatenco, Centro de Investigación y de Estudios Avanzados del Instituto Politecnico Nacional, 07360 Mexico City, MexicoTrypanosoma cruzi is the protozoan parasite that causes Chagas disease, which is considered by the World Health Organization to be a neglected tropical disease. Two drugs exist for the treatment of Chagas disease, nifurtimox and benznidazole; they are only effective in the acute phase, and a vaccine is currently not available. In this study, we used the recombinant enolase from T. cruzi H8 strain (MHOM/MX/1992/H8 Yucatán) (rTcENO) and its encoding DNA (pBKTcENO) to immunize mice and evaluate their protective effects in an experimental murine model of acute phase infection. Our results showed that mice vaccinated with rTcENO or its encoding DNA were able to generate typical specific antibodies (IgG1, IgG2a, and IgG2b), suggesting that a mixed Th1/Th2 immune response was induced. The parasite burden in the blood was reduced to 69.8% and 71% in mice vaccinated with rTcENO and pBKTcENO, respectively. The group vaccinated with rTcENO achieved 75% survival, in contrast to the group vaccinated with pBKTcENO that showed no survival in comparison to the control groups. Moreover, rTcENO immunization elevated the production of IFN-γ and IL-2 after the parasite challenge, suggesting that the Th1-type immune response was polarized. These results indicated that rTcENO could be used as a vaccine against Chagas disease.http://dx.doi.org/10.1155/2018/8964085 |
| spellingShingle | Minerva Arce-Fonseca María Cristina González-Vázquez Olivia Rodríguez-Morales Verónica Graullera-Rivera Alberto Aranda-Fraustro Pedro A. Reyes Alejandro Carabarin-Lima José Luis Rosales-Encina Recombinant Enolase of Trypanosoma cruzi as a Novel Vaccine Candidate against Chagas Disease in a Mouse Model of Acute Infection Journal of Immunology Research |
| title | Recombinant Enolase of Trypanosoma cruzi as a Novel Vaccine Candidate against Chagas Disease in a Mouse Model of Acute Infection |
| title_full | Recombinant Enolase of Trypanosoma cruzi as a Novel Vaccine Candidate against Chagas Disease in a Mouse Model of Acute Infection |
| title_fullStr | Recombinant Enolase of Trypanosoma cruzi as a Novel Vaccine Candidate against Chagas Disease in a Mouse Model of Acute Infection |
| title_full_unstemmed | Recombinant Enolase of Trypanosoma cruzi as a Novel Vaccine Candidate against Chagas Disease in a Mouse Model of Acute Infection |
| title_short | Recombinant Enolase of Trypanosoma cruzi as a Novel Vaccine Candidate against Chagas Disease in a Mouse Model of Acute Infection |
| title_sort | recombinant enolase of trypanosoma cruzi as a novel vaccine candidate against chagas disease in a mouse model of acute infection |
| url | http://dx.doi.org/10.1155/2018/8964085 |
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