A New Spectrophotometric Method for Determination of Selenium in Cosmetic and Pharmaceutical Preparations after Preconcentration with Cloud Point Extraction
A simple, rapid, and sensitive spectrophotometric method for the determination of trace amounts of selenium (IV) was described. In this method, all selenium spices reduced to selenium (IV) using 6 M HCl. Cloud point extraction was applied as a preconcentration method for spectrophotometric determina...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2011/729651 |
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| author | Mohammad Hosein Soruraddin Rouhollah Heydari Morteza Puladvand Mir Mehdi Zahedi |
| author_facet | Mohammad Hosein Soruraddin Rouhollah Heydari Morteza Puladvand Mir Mehdi Zahedi |
| author_sort | Mohammad Hosein Soruraddin |
| collection | DOAJ |
| description | A simple, rapid, and sensitive spectrophotometric method for the determination of trace amounts of selenium (IV) was described. In this method, all selenium spices reduced to selenium (IV) using 6 M HCl. Cloud point extraction was applied as a preconcentration method for spectrophotometric determination of selenium (IV) in aqueous solution. The proposed method is based on the complexation of Selenium (IV) with dithizone at pH < 1 in micellar medium (Triton X-100). After complexation with dithizone, the analyte was quantitatively extracted to the surfactant-rich phase by centrifugation and diluted to 5 mL with methanol. Since the absorption maxima of the complex (424 nm) and dithizone (434 nm) overlap, hence, the corrected absorbance, Acorr, was used to overcome the problem. With regard to the preconcentration, the tested parameters were the pH of the extraction, the concentration of the surfactant, the concentration of dithizone, and equilibration temperature and time. The detection limit is 4.4 ng mL-1; the relative standard deviation for six replicate measurements is 2.18% for 50 ng mL-1 of selenium. The procedure was applied successfully to the determination of selenium in two kinds of pharmaceutical samples. |
| format | Article |
| id | doaj-art-fa99199c644e4b75bd5fccd1515051d7 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-fa99199c644e4b75bd5fccd1515051d72025-02-03T00:59:40ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792011-01-01201110.1155/2011/729651729651A New Spectrophotometric Method for Determination of Selenium in Cosmetic and Pharmaceutical Preparations after Preconcentration with Cloud Point ExtractionMohammad Hosein Soruraddin0Rouhollah Heydari1Morteza Puladvand2Mir Mehdi Zahedi3Department of Analytical Chemistry, Faculty of Chemistry, Tabriz University, Tabriz 51664-14766, IranDepartment of Chemistry, Faculty of Science, Islamic Azad University, Khorramabad Branch, Khorramabad, IranDepartment of Analytical Chemistry, Faculty of Chemistry, Tabriz University, Tabriz 51664-14766, IranDepartment of Analytical Chemistry, Faculty of Chemistry, Razi University, Kermanshah, IranA simple, rapid, and sensitive spectrophotometric method for the determination of trace amounts of selenium (IV) was described. In this method, all selenium spices reduced to selenium (IV) using 6 M HCl. Cloud point extraction was applied as a preconcentration method for spectrophotometric determination of selenium (IV) in aqueous solution. The proposed method is based on the complexation of Selenium (IV) with dithizone at pH < 1 in micellar medium (Triton X-100). After complexation with dithizone, the analyte was quantitatively extracted to the surfactant-rich phase by centrifugation and diluted to 5 mL with methanol. Since the absorption maxima of the complex (424 nm) and dithizone (434 nm) overlap, hence, the corrected absorbance, Acorr, was used to overcome the problem. With regard to the preconcentration, the tested parameters were the pH of the extraction, the concentration of the surfactant, the concentration of dithizone, and equilibration temperature and time. The detection limit is 4.4 ng mL-1; the relative standard deviation for six replicate measurements is 2.18% for 50 ng mL-1 of selenium. The procedure was applied successfully to the determination of selenium in two kinds of pharmaceutical samples.http://dx.doi.org/10.1155/2011/729651 |
| spellingShingle | Mohammad Hosein Soruraddin Rouhollah Heydari Morteza Puladvand Mir Mehdi Zahedi A New Spectrophotometric Method for Determination of Selenium in Cosmetic and Pharmaceutical Preparations after Preconcentration with Cloud Point Extraction International Journal of Analytical Chemistry |
| title | A New Spectrophotometric Method for Determination of Selenium in Cosmetic and Pharmaceutical Preparations after Preconcentration with Cloud Point Extraction |
| title_full | A New Spectrophotometric Method for Determination of Selenium in Cosmetic and Pharmaceutical Preparations after Preconcentration with Cloud Point Extraction |
| title_fullStr | A New Spectrophotometric Method for Determination of Selenium in Cosmetic and Pharmaceutical Preparations after Preconcentration with Cloud Point Extraction |
| title_full_unstemmed | A New Spectrophotometric Method for Determination of Selenium in Cosmetic and Pharmaceutical Preparations after Preconcentration with Cloud Point Extraction |
| title_short | A New Spectrophotometric Method for Determination of Selenium in Cosmetic and Pharmaceutical Preparations after Preconcentration with Cloud Point Extraction |
| title_sort | new spectrophotometric method for determination of selenium in cosmetic and pharmaceutical preparations after preconcentration with cloud point extraction |
| url | http://dx.doi.org/10.1155/2011/729651 |
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