Molecular detection of bovine alphaherpesvirus 1 and 5 in the brain of cattle with and without neurological disease
The objective of the present study was to verify the presence of DNA of bovine alphaherpesvirus 5 (BoAHV5) and 1 (BoAHV1) in brain samples from healthy cattle slaughtered or that died naturally affected by neurological diseases. The healthy ones were beef cattle (n=30) raised in the north central m...
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Universidade Estadual de Londrina
2025-02-01
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| Series: | Semina: Ciências Agrárias |
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| Online Access: | https://www.ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/51483 |
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| author | Júlio Augusto Naylor Lisbôa José Renato Junqueira Borges Lilian Azevedo Figueiredo Gustavo Rodrigues Queiroz Rodrigo Azambuja Machado de Oliveira Bruna Stephani Ribeiro Janildo Ludolf Reis Junior Márcio Botelho de Castro Selwyn Arlington Headley Amauri Alcindo Alfieri |
| author_facet | Júlio Augusto Naylor Lisbôa José Renato Junqueira Borges Lilian Azevedo Figueiredo Gustavo Rodrigues Queiroz Rodrigo Azambuja Machado de Oliveira Bruna Stephani Ribeiro Janildo Ludolf Reis Junior Márcio Botelho de Castro Selwyn Arlington Headley Amauri Alcindo Alfieri |
| author_sort | Júlio Augusto Naylor Lisbôa |
| collection | DOAJ |
| description |
The objective of the present study was to verify the presence of DNA of bovine alphaherpesvirus 5 (BoAHV5) and 1 (BoAHV1) in brain samples from healthy cattle slaughtered or that died naturally affected by neurological diseases. The healthy ones were beef cattle (n=30) raised in the north central mesoregion from Paraná, southern Brazil. The sick cattle had a definite diagnosis of rabies (n=38), BoAHV encephalitis (n=25) and other neurological diseases (n=37). Polymerase chain reaction (PCR) assays were performed on fragments of the telencephalon, cerebellum and brain stem to confirm the presence of BoAHV5 and BoAHV1 DNA. Bovine alphaherpesviruses were confirmed in cases of BoAHV5 (n=22) and BoAHV1 (n=3) encephalitis. There were no observed coinfections. In rabid cattle, BoAHV5 was confirmed in eight (21%) and BoAHV1 in one (2.6%). BoAHV5 was also present in two (5.4%) cattle with other diseases (botulism and polioencephalomalacia), and in two (6.6%) healthy cattle. It can be concluded that, due to the possibility of latent infection, the presence of BoAHV5 or BoAHV1 DNA in the brain of cattle that died with neurological diseases may complicate the differential diagnosis in some cases.
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| format | Article |
| id | doaj-art-fa65e3b2719f4125a79bf8aed2d34b5c |
| institution | DOAJ |
| issn | 1676-546X 1679-0359 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj-art-fa65e3b2719f4125a79bf8aed2d34b5c2025-08-20T02:43:49ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592025-02-0146110.5433/1679-0359.2025v46n1p285Molecular detection of bovine alphaherpesvirus 1 and 5 in the brain of cattle with and without neurological diseaseJúlio Augusto Naylor Lisbôa0https://orcid.org/0000-0003-1180-703XJosé Renato Junqueira Borges1https://orcid.org/0000-0002-1338-4432Lilian Azevedo Figueiredo2https://orcid.org/0009-0006-7753-3384Gustavo Rodrigues Queiroz3https://orcid.org/0000-0001-8657-5056Rodrigo Azambuja Machado de Oliveira4https://orcid.org/0000-0003-0073-7005Bruna Stephani Ribeiro5https://orcid.org/0009-0004-8038-9171Janildo Ludolf Reis Junior6https://orcid.org/0000-0003-4978-788XMárcio Botelho de Castro7https://orcid.org/0000-0002-3996-0856Selwyn Arlington Headley8https://orcid.org/0000-0002-1614-0185Amauri Alcindo Alfieri9https://orcid.org/0000-0002-7024-4487Universidade Estadual de LondrinaUniversidade de Brasília Universidade de BrasíliaUniversidade Estadual de LondrinaUniversidade Tuiuti do ParanáUniversidade Estadual de LondrinaUniversidade Federal de Juiz de ForaUniversidade de Brasília Universidade Estadual de LondrinaUniversidade Estadual de Londrina The objective of the present study was to verify the presence of DNA of bovine alphaherpesvirus 5 (BoAHV5) and 1 (BoAHV1) in brain samples from healthy cattle slaughtered or that died naturally affected by neurological diseases. The healthy ones were beef cattle (n=30) raised in the north central mesoregion from Paraná, southern Brazil. The sick cattle had a definite diagnosis of rabies (n=38), BoAHV encephalitis (n=25) and other neurological diseases (n=37). Polymerase chain reaction (PCR) assays were performed on fragments of the telencephalon, cerebellum and brain stem to confirm the presence of BoAHV5 and BoAHV1 DNA. Bovine alphaherpesviruses were confirmed in cases of BoAHV5 (n=22) and BoAHV1 (n=3) encephalitis. There were no observed coinfections. In rabid cattle, BoAHV5 was confirmed in eight (21%) and BoAHV1 in one (2.6%). BoAHV5 was also present in two (5.4%) cattle with other diseases (botulism and polioencephalomalacia), and in two (6.6%) healthy cattle. It can be concluded that, due to the possibility of latent infection, the presence of BoAHV5 or BoAHV1 DNA in the brain of cattle that died with neurological diseases may complicate the differential diagnosis in some cases. https://www.ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/51483BoAHV5. Bovine rabiesDifferential diagnosisEncephalitisLatent infection. |
| spellingShingle | Júlio Augusto Naylor Lisbôa José Renato Junqueira Borges Lilian Azevedo Figueiredo Gustavo Rodrigues Queiroz Rodrigo Azambuja Machado de Oliveira Bruna Stephani Ribeiro Janildo Ludolf Reis Junior Márcio Botelho de Castro Selwyn Arlington Headley Amauri Alcindo Alfieri Molecular detection of bovine alphaherpesvirus 1 and 5 in the brain of cattle with and without neurological disease Semina: Ciências Agrárias BoAHV5. Bovine rabies Differential diagnosis Encephalitis Latent infection. |
| title | Molecular detection of bovine alphaherpesvirus 1 and 5 in the brain of cattle with and without neurological disease |
| title_full | Molecular detection of bovine alphaherpesvirus 1 and 5 in the brain of cattle with and without neurological disease |
| title_fullStr | Molecular detection of bovine alphaherpesvirus 1 and 5 in the brain of cattle with and without neurological disease |
| title_full_unstemmed | Molecular detection of bovine alphaherpesvirus 1 and 5 in the brain of cattle with and without neurological disease |
| title_short | Molecular detection of bovine alphaherpesvirus 1 and 5 in the brain of cattle with and without neurological disease |
| title_sort | molecular detection of bovine alphaherpesvirus 1 and 5 in the brain of cattle with and without neurological disease |
| topic | BoAHV5. Bovine rabies Differential diagnosis Encephalitis Latent infection. |
| url | https://www.ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/51483 |
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