Single-Photon Emission Computed Tomographic Imaging of the Early Time Course of Therapy-Induced Cell Death Using Technetium 99m Tricarbonyl His-Annexin A5 in a Colorectal Cancer Xenograft Model
As apoptosis occurs over an interval of time after administration of apoptosis-inducing therapy in tumors, the changes in technetium 99m ( 99m Tc)-tricarbonyl (CO) 3 His-annexin A5 (His-ann A5) accumulation over time were examined. Colo205-bearing mice were divided into six treatment groups: (1) con...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2012-03-01
|
| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2011.00034 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832544519810187264 |
|---|---|
| author | Christel Vangestel Christophe Van de Wiele Gilles Mees Koen Mertens Steven Staelens Chris Reutelingsperger Patrick Pauwels Nancy Van Damme Marc Peeters |
| author_facet | Christel Vangestel Christophe Van de Wiele Gilles Mees Koen Mertens Steven Staelens Chris Reutelingsperger Patrick Pauwels Nancy Van Damme Marc Peeters |
| author_sort | Christel Vangestel |
| collection | DOAJ |
| description | As apoptosis occurs over an interval of time after administration of apoptosis-inducing therapy in tumors, the changes in technetium 99m ( 99m Tc)-tricarbonyl (CO) 3 His-annexin A5 (His-ann A5) accumulation over time were examined. Colo205-bearing mice were divided into six treatment groups: (1) control, (2) 5-fluorouracil (5-FU; 250 mg/kg), (3) irinotecan (100 mg/kg), (4) oxaliplatin (30 mg/kg), (5) bevacizumab (5 mg/kg), and (6) panitumumab (6 mg/kg). 99m Tc-(CO) 3 His-ann A5 was injected 4, 8, 12, 24, and 48 hours posttreatment, and micro–single-photon emission computed tomography was performed. Immunostaining of caspase-3 (apoptosis), survivin (antiapoptosis), and LC3-II (autophagy marker) was also performed. Different dynamics of 99m Tc-(CO) 3 His-ann A5 uptake were observed in this colorectal cancer xenograft model, in response to a single dose of three different chemotherapeutics (5-FU, irinotecan, and oxaliplatin). Bevacizumab-treated mice showed no increased uptake of the radiotracer, and a peak of 99m Tc-(CO) 3 His-ann A5 uptake in panitumumab-treated mice was observed 24 hours posttreatment, as confirmed by caspase-3 immunostaining. For irinotecan-, oxaliplatin-, and bevacizumab-treated tumors, a significant correlation was established between the radiotracer uptake and caspase-3 immunostaining ( r = .8, p < .05; r = .9, p < .001; r = .9, p < .001, respectively). For 5-FU- and panitumumabtreated mice, the correlation coefficients were r = .7 ( p = .18) and r = .7 ( p = .19), respectively. Optimal timing of annexin A5 imaging after the start of different treatments in the Colo205 model was determined. |
| format | Article |
| id | doaj-art-fa56870676594d7e9dc080af89727314 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2012-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-fa56870676594d7e9dc080af897273142025-02-03T10:12:57ZengSAGE PublishingMolecular Imaging1536-01212012-03-011110.2310/7290.2011.0003410.2310_7290.2011.00034Single-Photon Emission Computed Tomographic Imaging of the Early Time Course of Therapy-Induced Cell Death Using Technetium 99m Tricarbonyl His-Annexin A5 in a Colorectal Cancer Xenograft ModelChristel VangestelChristophe Van de WieleGilles MeesKoen MertensSteven StaelensChris ReutelingspergerPatrick PauwelsNancy Van DammeMarc PeetersAs apoptosis occurs over an interval of time after administration of apoptosis-inducing therapy in tumors, the changes in technetium 99m ( 99m Tc)-tricarbonyl (CO) 3 His-annexin A5 (His-ann A5) accumulation over time were examined. Colo205-bearing mice were divided into six treatment groups: (1) control, (2) 5-fluorouracil (5-FU; 250 mg/kg), (3) irinotecan (100 mg/kg), (4) oxaliplatin (30 mg/kg), (5) bevacizumab (5 mg/kg), and (6) panitumumab (6 mg/kg). 99m Tc-(CO) 3 His-ann A5 was injected 4, 8, 12, 24, and 48 hours posttreatment, and micro–single-photon emission computed tomography was performed. Immunostaining of caspase-3 (apoptosis), survivin (antiapoptosis), and LC3-II (autophagy marker) was also performed. Different dynamics of 99m Tc-(CO) 3 His-ann A5 uptake were observed in this colorectal cancer xenograft model, in response to a single dose of three different chemotherapeutics (5-FU, irinotecan, and oxaliplatin). Bevacizumab-treated mice showed no increased uptake of the radiotracer, and a peak of 99m Tc-(CO) 3 His-ann A5 uptake in panitumumab-treated mice was observed 24 hours posttreatment, as confirmed by caspase-3 immunostaining. For irinotecan-, oxaliplatin-, and bevacizumab-treated tumors, a significant correlation was established between the radiotracer uptake and caspase-3 immunostaining ( r = .8, p < .05; r = .9, p < .001; r = .9, p < .001, respectively). For 5-FU- and panitumumabtreated mice, the correlation coefficients were r = .7 ( p = .18) and r = .7 ( p = .19), respectively. Optimal timing of annexin A5 imaging after the start of different treatments in the Colo205 model was determined.https://doi.org/10.2310/7290.2011.00034 |
| spellingShingle | Christel Vangestel Christophe Van de Wiele Gilles Mees Koen Mertens Steven Staelens Chris Reutelingsperger Patrick Pauwels Nancy Van Damme Marc Peeters Single-Photon Emission Computed Tomographic Imaging of the Early Time Course of Therapy-Induced Cell Death Using Technetium 99m Tricarbonyl His-Annexin A5 in a Colorectal Cancer Xenograft Model Molecular Imaging |
| title | Single-Photon Emission Computed Tomographic Imaging of the Early Time Course of Therapy-Induced Cell Death Using Technetium 99m Tricarbonyl His-Annexin A5 in a Colorectal Cancer Xenograft Model |
| title_full | Single-Photon Emission Computed Tomographic Imaging of the Early Time Course of Therapy-Induced Cell Death Using Technetium 99m Tricarbonyl His-Annexin A5 in a Colorectal Cancer Xenograft Model |
| title_fullStr | Single-Photon Emission Computed Tomographic Imaging of the Early Time Course of Therapy-Induced Cell Death Using Technetium 99m Tricarbonyl His-Annexin A5 in a Colorectal Cancer Xenograft Model |
| title_full_unstemmed | Single-Photon Emission Computed Tomographic Imaging of the Early Time Course of Therapy-Induced Cell Death Using Technetium 99m Tricarbonyl His-Annexin A5 in a Colorectal Cancer Xenograft Model |
| title_short | Single-Photon Emission Computed Tomographic Imaging of the Early Time Course of Therapy-Induced Cell Death Using Technetium 99m Tricarbonyl His-Annexin A5 in a Colorectal Cancer Xenograft Model |
| title_sort | single photon emission computed tomographic imaging of the early time course of therapy induced cell death using technetium 99m tricarbonyl his annexin a5 in a colorectal cancer xenograft model |
| url | https://doi.org/10.2310/7290.2011.00034 |
| work_keys_str_mv | AT christelvangestel singlephotonemissioncomputedtomographicimagingoftheearlytimecourseoftherapyinducedcelldeathusingtechnetium99mtricarbonylhisannexina5inacolorectalcancerxenograftmodel AT christophevandewiele singlephotonemissioncomputedtomographicimagingoftheearlytimecourseoftherapyinducedcelldeathusingtechnetium99mtricarbonylhisannexina5inacolorectalcancerxenograftmodel AT gillesmees singlephotonemissioncomputedtomographicimagingoftheearlytimecourseoftherapyinducedcelldeathusingtechnetium99mtricarbonylhisannexina5inacolorectalcancerxenograftmodel AT koenmertens singlephotonemissioncomputedtomographicimagingoftheearlytimecourseoftherapyinducedcelldeathusingtechnetium99mtricarbonylhisannexina5inacolorectalcancerxenograftmodel AT stevenstaelens singlephotonemissioncomputedtomographicimagingoftheearlytimecourseoftherapyinducedcelldeathusingtechnetium99mtricarbonylhisannexina5inacolorectalcancerxenograftmodel AT chrisreutelingsperger singlephotonemissioncomputedtomographicimagingoftheearlytimecourseoftherapyinducedcelldeathusingtechnetium99mtricarbonylhisannexina5inacolorectalcancerxenograftmodel AT patrickpauwels singlephotonemissioncomputedtomographicimagingoftheearlytimecourseoftherapyinducedcelldeathusingtechnetium99mtricarbonylhisannexina5inacolorectalcancerxenograftmodel AT nancyvandamme singlephotonemissioncomputedtomographicimagingoftheearlytimecourseoftherapyinducedcelldeathusingtechnetium99mtricarbonylhisannexina5inacolorectalcancerxenograftmodel AT marcpeeters singlephotonemissioncomputedtomographicimagingoftheearlytimecourseoftherapyinducedcelldeathusingtechnetium99mtricarbonylhisannexina5inacolorectalcancerxenograftmodel |