Induced mesenchymal stem cells generated from periodontal ligament fibroblast for regenerative therapy
Bone fractures and bone loss represent significant global health challenges, with their incidence rising due to an aging population. Despite autologous bone grafts remain the gold standard for treatment, challenges such as limited bone availability, immune reactions, and the risk of infectious disea...
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Frontiers Media S.A.
2025-02-01
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| Series: | Experimental Biology and Medicine |
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| Online Access: | https://www.ebm-journal.org/articles/10.3389/ebm.2025.10342/full |
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| author | Hemanathan Vembuli Sheeja Rajasingh Patrick Nabholz Jefferson Guenther Brian R. Morrow Margaret M. Taylor Marziyeh Aghazadeh Vinoth Sigamani Johnson Rajasingh Johnson Rajasingh Johnson Rajasingh |
| author_facet | Hemanathan Vembuli Sheeja Rajasingh Patrick Nabholz Jefferson Guenther Brian R. Morrow Margaret M. Taylor Marziyeh Aghazadeh Vinoth Sigamani Johnson Rajasingh Johnson Rajasingh Johnson Rajasingh |
| author_sort | Hemanathan Vembuli |
| collection | DOAJ |
| description | Bone fractures and bone loss represent significant global health challenges, with their incidence rising due to an aging population. Despite autologous bone grafts remain the gold standard for treatment, challenges such as limited bone availability, immune reactions, and the risk of infectious disease transmission have driven the search for alternative cell-based therapies for bone regeneration. Stem cells derived from oral tissues and umbilical cord mesenchymal stem cells (MSCs) have shown potential in both preclinical and clinical studies for bone tissue regeneration. However, their limited differentiation capacity and wound healing abilities necessitate the exploration of alternative cell sources. In this study, we generated induced pluripotent stem cells (iPSCs) using a safe, nonviral and mRNA-based approach from human periodontal ligament fibroblasts (PDLF), an easily accessible cell source. These iPSCs were subsequently differentiated into MSCs, referred to as induced MSCs (iMSCs). The resulting iMSCs were homogeneous, highly proliferative, and possessed anti-inflammatory properties, suggesting their potential as a superior alternative to traditional MSCs for regenerative therapy. These iMSCs demonstrated trilineage differentiation potential, giving rise to osteocytes, chondrocytes, and adipocytes. The iMSC-derived osteocytes (iOSTs) were homogeneous, patient-specific and showed excellent attachment and growth on commercial collagen-based membranes, highlighting their suitability for bone tissue regeneration applications. Given their promising characteristics compared to traditional MSCs, PDLF-derived iMSCs are strong candidates for future clinical studies in bone regeneration and other regenerative dental therapies. |
| format | Article |
| id | doaj-art-fa45d7415b044db583a7a9e6c298af88 |
| institution | Kabale University |
| issn | 1535-3699 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Experimental Biology and Medicine |
| spelling | doaj-art-fa45d7415b044db583a7a9e6c298af882025-02-03T04:10:58ZengFrontiers Media S.A.Experimental Biology and Medicine1535-36992025-02-0125010.3389/ebm.2025.1034210342Induced mesenchymal stem cells generated from periodontal ligament fibroblast for regenerative therapyHemanathan Vembuli0Sheeja Rajasingh1Patrick Nabholz2Jefferson Guenther3Brian R. Morrow4Margaret M. Taylor5Marziyeh Aghazadeh6Vinoth Sigamani7Johnson Rajasingh8Johnson Rajasingh9Johnson Rajasingh10Department of Bioscience Research, University of Tennessee Health Science Center, Memphis, TN, United StatesDepartment of Bioscience Research, University of Tennessee Health Science Center, Memphis, TN, United StatesDepartment of Bioscience Research, University of Tennessee Health Science Center, Memphis, TN, United StatesDepartment of Bioscience Research, University of Tennessee Health Science Center, Memphis, TN, United StatesDepartment of Bioscience Research, University of Tennessee Health Science Center, Memphis, TN, United StatesDepartment of Bioscience Research, University of Tennessee Health Science Center, Memphis, TN, United StatesDepartment of Bioscience Research, University of Tennessee Health Science Center, Memphis, TN, United StatesDepartment of Bioscience Research, University of Tennessee Health Science Center, Memphis, TN, United StatesDepartment of Bioscience Research, University of Tennessee Health Science Center, Memphis, TN, United StatesDepartment of Medicine-Cardiology, University of Tennessee Health Science Center, Memphis, TN, United StatesDepartment of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, United StatesBone fractures and bone loss represent significant global health challenges, with their incidence rising due to an aging population. Despite autologous bone grafts remain the gold standard for treatment, challenges such as limited bone availability, immune reactions, and the risk of infectious disease transmission have driven the search for alternative cell-based therapies for bone regeneration. Stem cells derived from oral tissues and umbilical cord mesenchymal stem cells (MSCs) have shown potential in both preclinical and clinical studies for bone tissue regeneration. However, their limited differentiation capacity and wound healing abilities necessitate the exploration of alternative cell sources. In this study, we generated induced pluripotent stem cells (iPSCs) using a safe, nonviral and mRNA-based approach from human periodontal ligament fibroblasts (PDLF), an easily accessible cell source. These iPSCs were subsequently differentiated into MSCs, referred to as induced MSCs (iMSCs). The resulting iMSCs were homogeneous, highly proliferative, and possessed anti-inflammatory properties, suggesting their potential as a superior alternative to traditional MSCs for regenerative therapy. These iMSCs demonstrated trilineage differentiation potential, giving rise to osteocytes, chondrocytes, and adipocytes. The iMSC-derived osteocytes (iOSTs) were homogeneous, patient-specific and showed excellent attachment and growth on commercial collagen-based membranes, highlighting their suitability for bone tissue regeneration applications. Given their promising characteristics compared to traditional MSCs, PDLF-derived iMSCs are strong candidates for future clinical studies in bone regeneration and other regenerative dental therapies.https://www.ebm-journal.org/articles/10.3389/ebm.2025.10342/fullmesenchymal stem cellsinduced pluripotent stem cellsdifferentiationosteocytesregenerative therapy |
| spellingShingle | Hemanathan Vembuli Sheeja Rajasingh Patrick Nabholz Jefferson Guenther Brian R. Morrow Margaret M. Taylor Marziyeh Aghazadeh Vinoth Sigamani Johnson Rajasingh Johnson Rajasingh Johnson Rajasingh Induced mesenchymal stem cells generated from periodontal ligament fibroblast for regenerative therapy Experimental Biology and Medicine mesenchymal stem cells induced pluripotent stem cells differentiation osteocytes regenerative therapy |
| title | Induced mesenchymal stem cells generated from periodontal ligament fibroblast for regenerative therapy |
| title_full | Induced mesenchymal stem cells generated from periodontal ligament fibroblast for regenerative therapy |
| title_fullStr | Induced mesenchymal stem cells generated from periodontal ligament fibroblast for regenerative therapy |
| title_full_unstemmed | Induced mesenchymal stem cells generated from periodontal ligament fibroblast for regenerative therapy |
| title_short | Induced mesenchymal stem cells generated from periodontal ligament fibroblast for regenerative therapy |
| title_sort | induced mesenchymal stem cells generated from periodontal ligament fibroblast for regenerative therapy |
| topic | mesenchymal stem cells induced pluripotent stem cells differentiation osteocytes regenerative therapy |
| url | https://www.ebm-journal.org/articles/10.3389/ebm.2025.10342/full |
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