Assessment of Peri-Implant Soft Tissue Reactions in Patients with Systemic Conditions: An In Vitro Cellular Analysis
Background: Peri-implant soft tissue health is crucial for the long-term success of dental implants. Patients with systemic conditions, such as diabetes mellitus and cardiovascular diseases, are at higher risk for peri-implant complications. This study aimed to assess the cellular responses of peri-...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-05-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
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| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_1865_24 |
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| author | Monica Jaideep Mahajani Aleena Paulson Ripal Parikh Pradnya Wagh Rashmi Laddha Minerva Singh Miral Mehta |
| author_facet | Monica Jaideep Mahajani Aleena Paulson Ripal Parikh Pradnya Wagh Rashmi Laddha Minerva Singh Miral Mehta |
| author_sort | Monica Jaideep Mahajani |
| collection | DOAJ |
| description | Background:
Peri-implant soft tissue health is crucial for the long-term success of dental implants. Patients with systemic conditions, such as diabetes mellitus and cardiovascular diseases, are at higher risk for peri-implant complications. This study aimed to assess the cellular responses of peri-implant soft tissues in vitro, simulating conditions influenced by systemic diseases, to better understand the potential impact of these conditions on implant success.
Materials and Methods:
An in vitro study was conducted using human gingival fibroblasts exposed to simulated inflammatory conditions representative of diabetes (high glucose environment) and cardiovascular diseases (increased proinflammatory cytokines). Titanium discs with treated surfaces were used to mimic implant surfaces. Cellular proliferation, adhesion, and cytokine expression were evaluated using MTT assay, fluorescence microscopy, and ELISA, respectively. Experimental groups included Group 1: Healthy control, Group 2: High-glucose condition, Group 3: Proinflammatory cytokine condition, and Group 4: Combined high-glucose and proinflammatory cytokine condition.
Results:
Fibroblast proliferation rates were significantly reduced in Groups 2 (50%) and 3 (55%) compared to Group 1 (85%). Adhesion strength was also compromised in Groups 2 (60%) and 3 (65%), with the lowest observed in Group 4 (40%). Cytokine analysis revealed elevated levels of IL-6 and TNF-α in Groups 3 (IL-6: 150 pg/mL, TNF-α: 200 pg/mL) and 4 (IL-6: 180 pg/mL, TNF-a: 250 pg/mL) compared to the control (IL-6: 50 pg/mL, TNF-α: 60 pg/mL).
Conclusion:
Systemic conditions significantly impair peri-implant soft tissue cellular responses, including proliferation, adhesion, and inflammation regulation. These findings underscore the importance of tailored peri-implant management strategies for patients with systemic diseases to ensure implant longevity. |
| format | Article |
| id | doaj-art-fa41b8d98b894d7ea5a3ecb58c8f08e8 |
| institution | OA Journals |
| issn | 0976-4879 0975-7406 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj-art-fa41b8d98b894d7ea5a3ecb58c8f08e82025-08-20T02:34:43ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062025-05-0117Suppl 1S586S58810.4103/jpbs.jpbs_1865_24Assessment of Peri-Implant Soft Tissue Reactions in Patients with Systemic Conditions: An In Vitro Cellular AnalysisMonica Jaideep MahajaniAleena PaulsonRipal ParikhPradnya WaghRashmi LaddhaMinerva SinghMiral MehtaBackground: Peri-implant soft tissue health is crucial for the long-term success of dental implants. Patients with systemic conditions, such as diabetes mellitus and cardiovascular diseases, are at higher risk for peri-implant complications. This study aimed to assess the cellular responses of peri-implant soft tissues in vitro, simulating conditions influenced by systemic diseases, to better understand the potential impact of these conditions on implant success. Materials and Methods: An in vitro study was conducted using human gingival fibroblasts exposed to simulated inflammatory conditions representative of diabetes (high glucose environment) and cardiovascular diseases (increased proinflammatory cytokines). Titanium discs with treated surfaces were used to mimic implant surfaces. Cellular proliferation, adhesion, and cytokine expression were evaluated using MTT assay, fluorescence microscopy, and ELISA, respectively. Experimental groups included Group 1: Healthy control, Group 2: High-glucose condition, Group 3: Proinflammatory cytokine condition, and Group 4: Combined high-glucose and proinflammatory cytokine condition. Results: Fibroblast proliferation rates were significantly reduced in Groups 2 (50%) and 3 (55%) compared to Group 1 (85%). Adhesion strength was also compromised in Groups 2 (60%) and 3 (65%), with the lowest observed in Group 4 (40%). Cytokine analysis revealed elevated levels of IL-6 and TNF-α in Groups 3 (IL-6: 150 pg/mL, TNF-α: 200 pg/mL) and 4 (IL-6: 180 pg/mL, TNF-a: 250 pg/mL) compared to the control (IL-6: 50 pg/mL, TNF-α: 60 pg/mL). Conclusion: Systemic conditions significantly impair peri-implant soft tissue cellular responses, including proliferation, adhesion, and inflammation regulation. These findings underscore the importance of tailored peri-implant management strategies for patients with systemic diseases to ensure implant longevity.https://journals.lww.com/10.4103/jpbs.jpbs_1865_24cardiovascular diseasescellular responsesdental implantsdiabetes mellitusin vitro analysisperi-implant soft tissuesystemic conditions |
| spellingShingle | Monica Jaideep Mahajani Aleena Paulson Ripal Parikh Pradnya Wagh Rashmi Laddha Minerva Singh Miral Mehta Assessment of Peri-Implant Soft Tissue Reactions in Patients with Systemic Conditions: An In Vitro Cellular Analysis Journal of Pharmacy and Bioallied Sciences cardiovascular diseases cellular responses dental implants diabetes mellitus in vitro analysis peri-implant soft tissue systemic conditions |
| title | Assessment of Peri-Implant Soft Tissue Reactions in Patients with Systemic Conditions: An In Vitro Cellular Analysis |
| title_full | Assessment of Peri-Implant Soft Tissue Reactions in Patients with Systemic Conditions: An In Vitro Cellular Analysis |
| title_fullStr | Assessment of Peri-Implant Soft Tissue Reactions in Patients with Systemic Conditions: An In Vitro Cellular Analysis |
| title_full_unstemmed | Assessment of Peri-Implant Soft Tissue Reactions in Patients with Systemic Conditions: An In Vitro Cellular Analysis |
| title_short | Assessment of Peri-Implant Soft Tissue Reactions in Patients with Systemic Conditions: An In Vitro Cellular Analysis |
| title_sort | assessment of peri implant soft tissue reactions in patients with systemic conditions an in vitro cellular analysis |
| topic | cardiovascular diseases cellular responses dental implants diabetes mellitus in vitro analysis peri-implant soft tissue systemic conditions |
| url | https://journals.lww.com/10.4103/jpbs.jpbs_1865_24 |
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