Suaveolic Acid: A Potent Phytotoxic Substance of Hyptis suaveolens
Hyptis suaveolens (Lamiaceae) is an exotic invasive plant in many countries. Earlier studies reported that the aqueous, methanol, and aqueous methanol extract of H. suaveolens and its residues have phytotoxic properties. However, to date, the phytotoxic substances of this plant have not been reporte...
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| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/425942 |
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| author | A. K. M. Mominul Islam Osamu Ohno Kiyotake Suenaga Hisashi Kato-Noguchi |
| author_facet | A. K. M. Mominul Islam Osamu Ohno Kiyotake Suenaga Hisashi Kato-Noguchi |
| author_sort | A. K. M. Mominul Islam |
| collection | DOAJ |
| description | Hyptis suaveolens (Lamiaceae) is an exotic invasive plant in many countries. Earlier studies reported that the aqueous, methanol, and aqueous methanol extract of H. suaveolens and its residues have phytotoxic properties. However, to date, the phytotoxic substances of this plant have not been reported. Therefore, the objectives of this study were isolation and identification of phytotoxic substances of H. suaveolens. Aqueous methanol extract of this plant was purified by several chromatographic runs through bioassay guided fractionation using garden cress (Lepidium sativum) as a test plant. Final purification of a phytotoxic substance was achieved by reverse phase HPLC and characterized as 14α-hydroxy-13β-abiet-8-en-18-oic acid (suaveolic acid) by high-resolution ESI-MS, 1H-,13C-NMR, CD, and specific rotation. Suaveolic acid inhibited the shoot growth of garden cress, lettuce (Lactuca sativa), Italian ryegrass (Lolium multiflorum), and barnyard grass (Echinochloa crus-galli) at concentrations greater than 30 µM. Root growth of all but lettuce was also inhibited at concentrations greater than 30 µM. The inhibitory activities were concentration dependent. Concentrations required for 50% growth inhibition of suaveolic acid for those test plant species were ranged from 76 to 1155 µM. Therefore, suaveolic acid is phytotoxic and may be responsible for the phytotoxicity of H. suaveolens plant extracts. |
| format | Article |
| id | doaj-art-fa11e547f3a7497998d9bad0af35ec9a |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | The Scientific World Journal |
| spelling | doaj-art-fa11e547f3a7497998d9bad0af35ec9a2025-02-03T05:59:27ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/425942425942Suaveolic Acid: A Potent Phytotoxic Substance of Hyptis suaveolensA. K. M. Mominul Islam0Osamu Ohno1Kiyotake Suenaga2Hisashi Kato-Noguchi3Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki, Kagawa 761-0795, JapanDepartment of Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku, Yokohama 223-8522, JapanDepartment of Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku, Yokohama 223-8522, JapanDepartment of Applied Biological Science, Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki, Kagawa 761-0795, JapanHyptis suaveolens (Lamiaceae) is an exotic invasive plant in many countries. Earlier studies reported that the aqueous, methanol, and aqueous methanol extract of H. suaveolens and its residues have phytotoxic properties. However, to date, the phytotoxic substances of this plant have not been reported. Therefore, the objectives of this study were isolation and identification of phytotoxic substances of H. suaveolens. Aqueous methanol extract of this plant was purified by several chromatographic runs through bioassay guided fractionation using garden cress (Lepidium sativum) as a test plant. Final purification of a phytotoxic substance was achieved by reverse phase HPLC and characterized as 14α-hydroxy-13β-abiet-8-en-18-oic acid (suaveolic acid) by high-resolution ESI-MS, 1H-,13C-NMR, CD, and specific rotation. Suaveolic acid inhibited the shoot growth of garden cress, lettuce (Lactuca sativa), Italian ryegrass (Lolium multiflorum), and barnyard grass (Echinochloa crus-galli) at concentrations greater than 30 µM. Root growth of all but lettuce was also inhibited at concentrations greater than 30 µM. The inhibitory activities were concentration dependent. Concentrations required for 50% growth inhibition of suaveolic acid for those test plant species were ranged from 76 to 1155 µM. Therefore, suaveolic acid is phytotoxic and may be responsible for the phytotoxicity of H. suaveolens plant extracts.http://dx.doi.org/10.1155/2014/425942 |
| spellingShingle | A. K. M. Mominul Islam Osamu Ohno Kiyotake Suenaga Hisashi Kato-Noguchi Suaveolic Acid: A Potent Phytotoxic Substance of Hyptis suaveolens The Scientific World Journal |
| title | Suaveolic Acid: A Potent Phytotoxic Substance of Hyptis suaveolens |
| title_full | Suaveolic Acid: A Potent Phytotoxic Substance of Hyptis suaveolens |
| title_fullStr | Suaveolic Acid: A Potent Phytotoxic Substance of Hyptis suaveolens |
| title_full_unstemmed | Suaveolic Acid: A Potent Phytotoxic Substance of Hyptis suaveolens |
| title_short | Suaveolic Acid: A Potent Phytotoxic Substance of Hyptis suaveolens |
| title_sort | suaveolic acid a potent phytotoxic substance of hyptis suaveolens |
| url | http://dx.doi.org/10.1155/2014/425942 |
| work_keys_str_mv | AT akmmominulislam suaveolicacidapotentphytotoxicsubstanceofhyptissuaveolens AT osamuohno suaveolicacidapotentphytotoxicsubstanceofhyptissuaveolens AT kiyotakesuenaga suaveolicacidapotentphytotoxicsubstanceofhyptissuaveolens AT hisashikatonoguchi suaveolicacidapotentphytotoxicsubstanceofhyptissuaveolens |