Diagnosis of Oral Hairy Leukoplakia: The Importance of EBV In Situ Hybridization
Oral hairy leukoplakia (OHL) is caused by the Epstein-Barr virus (EBV), which has been related to HIV infection. In situ hybridization (ISH) is the gold-standard diagnosis of OHL, but some authors believe in the possibility of performing the diagnosis based on clinical basis. The aim of this study i...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2017/3457479 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550738786516992 |
|---|---|
| author | Luana L. Martins José Henrique F. Rosseto Natália Silva Andrade Juliana Bertoldi Franco Paulo Henrique Braz-Silva Karem L. Ortega |
| author_facet | Luana L. Martins José Henrique F. Rosseto Natália Silva Andrade Juliana Bertoldi Franco Paulo Henrique Braz-Silva Karem L. Ortega |
| author_sort | Luana L. Martins |
| collection | DOAJ |
| description | Oral hairy leukoplakia (OHL) is caused by the Epstein-Barr virus (EBV), which has been related to HIV infection. In situ hybridization (ISH) is the gold-standard diagnosis of OHL, but some authors believe in the possibility of performing the diagnosis based on clinical basis. The aim of this study is diagnose incipient lesions of OHL by EBV ISH of HIV-infected patients and the possible correlations with clinical characteristics of the patients. Ninety-four patients were examined and those presenting with clinical lesions compatible to OHL were submitted to biopsy prior to EBV ISH. Twenty-eight patients had lesions clinically compatible to the diagnosis of OHL, but only 20 lesions were confirmed by EBV ISH. The patients with OHL had a mean age of 41.9 years and were HIV-infected for 11.2 years, on average, including CD4 count of 504.7 cells/mm3 and log10 viral load = 1.1. Among the quantitative variables, there was a statistically significant correlation with age only (P=0.030). In conclusion, the presence of OHL in patients with HIV/AIDS results in changes in the epidemiological characteristics of the disease, and this fact allied with subtle clinical-morphological features makes clinical diagnosis very difficult. Therefore, EBV ISH is important for a definitive diagnosis of OHL. |
| format | Article |
| id | doaj-art-f9dc622179ae4c32a69a0e6c1009ac18 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-f9dc622179ae4c32a69a0e6c1009ac182025-02-03T06:06:02ZengWileyInternational Journal of Dentistry1687-87281687-87362017-01-01201710.1155/2017/34574793457479Diagnosis of Oral Hairy Leukoplakia: The Importance of EBV In Situ HybridizationLuana L. Martins0José Henrique F. Rosseto1Natália Silva Andrade2Juliana Bertoldi Franco3Paulo Henrique Braz-Silva4Karem L. Ortega5Special Care Dentistry Center, Division of Oral and Maxillofacial Pathology, Department of Stomatology, School of Dentistry, University of Sao Paulo, Sao Paulo, SP, BrazilSpecial Care Dentistry Center, Division of Oral and Maxillofacial Pathology, Department of Stomatology, School of Dentistry, University of Sao Paulo, Sao Paulo, SP, BrazilSpecial Care Dentistry Center, Division of Oral and Maxillofacial Pathology, Department of Stomatology, School of Dentistry, University of Sao Paulo, Sao Paulo, SP, BrazilSpecial Care Dentistry Center, Division of Oral and Maxillofacial Pathology, Department of Stomatology, School of Dentistry, University of Sao Paulo, Sao Paulo, SP, BrazilDivision of General Pathology, Department of Stomatology, School of Dentistry, University of Sao Paulo, Sao Paulo, SP, BrazilSpecial Care Dentistry Center, Division of Oral and Maxillofacial Pathology, Department of Stomatology, School of Dentistry, University of Sao Paulo, Sao Paulo, SP, BrazilOral hairy leukoplakia (OHL) is caused by the Epstein-Barr virus (EBV), which has been related to HIV infection. In situ hybridization (ISH) is the gold-standard diagnosis of OHL, but some authors believe in the possibility of performing the diagnosis based on clinical basis. The aim of this study is diagnose incipient lesions of OHL by EBV ISH of HIV-infected patients and the possible correlations with clinical characteristics of the patients. Ninety-four patients were examined and those presenting with clinical lesions compatible to OHL were submitted to biopsy prior to EBV ISH. Twenty-eight patients had lesions clinically compatible to the diagnosis of OHL, but only 20 lesions were confirmed by EBV ISH. The patients with OHL had a mean age of 41.9 years and were HIV-infected for 11.2 years, on average, including CD4 count of 504.7 cells/mm3 and log10 viral load = 1.1. Among the quantitative variables, there was a statistically significant correlation with age only (P=0.030). In conclusion, the presence of OHL in patients with HIV/AIDS results in changes in the epidemiological characteristics of the disease, and this fact allied with subtle clinical-morphological features makes clinical diagnosis very difficult. Therefore, EBV ISH is important for a definitive diagnosis of OHL.http://dx.doi.org/10.1155/2017/3457479 |
| spellingShingle | Luana L. Martins José Henrique F. Rosseto Natália Silva Andrade Juliana Bertoldi Franco Paulo Henrique Braz-Silva Karem L. Ortega Diagnosis of Oral Hairy Leukoplakia: The Importance of EBV In Situ Hybridization International Journal of Dentistry |
| title | Diagnosis of Oral Hairy Leukoplakia: The Importance of EBV In Situ Hybridization |
| title_full | Diagnosis of Oral Hairy Leukoplakia: The Importance of EBV In Situ Hybridization |
| title_fullStr | Diagnosis of Oral Hairy Leukoplakia: The Importance of EBV In Situ Hybridization |
| title_full_unstemmed | Diagnosis of Oral Hairy Leukoplakia: The Importance of EBV In Situ Hybridization |
| title_short | Diagnosis of Oral Hairy Leukoplakia: The Importance of EBV In Situ Hybridization |
| title_sort | diagnosis of oral hairy leukoplakia the importance of ebv in situ hybridization |
| url | http://dx.doi.org/10.1155/2017/3457479 |
| work_keys_str_mv | AT luanalmartins diagnosisoforalhairyleukoplakiatheimportanceofebvinsituhybridization AT josehenriquefrosseto diagnosisoforalhairyleukoplakiatheimportanceofebvinsituhybridization AT nataliasilvaandrade diagnosisoforalhairyleukoplakiatheimportanceofebvinsituhybridization AT julianabertoldifranco diagnosisoforalhairyleukoplakiatheimportanceofebvinsituhybridization AT paulohenriquebrazsilva diagnosisoforalhairyleukoplakiatheimportanceofebvinsituhybridization AT karemlortega diagnosisoforalhairyleukoplakiatheimportanceofebvinsituhybridization |