Species identification based on the fecal DNA samples of the Caprinae
Fecal analysis is a useful tool for studying the species identity of rare mammals. The possibility of using non-invasive biological materials in molecular genetic studies of rare bovids is shown, using the example of the markhor and Siberian ibex of Uzbekistan. Field work including noninvasive genet...
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| Format: | Article |
| Language: | English |
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Altai State University
2024-12-01
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| Series: | Acta Biologica Sibirica |
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| Online Access: | http://journal.asu.ru/biol/article/view/16502 |
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| author | Abdurakhim E. Kuchboev Oybek O. Amirov Makhamadi B. Abramatov Bakhtiyor H. Ruziev Mehmonjon Kh. Egamberdiyev Rokhatoy R. Karimova |
| author_facet | Abdurakhim E. Kuchboev Oybek O. Amirov Makhamadi B. Abramatov Bakhtiyor H. Ruziev Mehmonjon Kh. Egamberdiyev Rokhatoy R. Karimova |
| author_sort | Abdurakhim E. Kuchboev |
| collection | DOAJ |
| description | Fecal analysis is a useful tool for studying the species identity of rare mammals. The possibility of using non-invasive biological materials in molecular genetic studies of rare bovids is shown, using the example of the markhor and Siberian ibex of Uzbekistan. Field work including noninvasive genetic sampling collection was carried out in the study area in spring and autumn 2022-2023 in the Hissar, Surkhan State Reserves and Ugam-Chatkal State National Natural Park and Termez zoo in Uzbekistan. We used species-specific 16S rRNA mitochondrial gene fragments for polymerase chain reaction amplification for species identification. The results of the molecular analysis with the 16S rRNA mitochondrial gene allowed the identification of Capra sibirica, C. falconeri and C. hircus belonging to the subfamily Caprinae using a noninvasive genetic sampling method. This method is quite easy to use, while avoiding direct contact with the animal, which minimizes the degree of impact on the object being studied and does not require significant material and labor costs for researchers. We believe that noninvasive genetic sampling is emerging as one of the most effective and accurate methods for estimating the population size of animals, and we recommend considering this approach for endangered and rare species. The protocol developed could be a valuable tool in the management and conservation of the Capra species occurring on the Uzbekistan. |
| format | Article |
| id | doaj-art-f9d57e88f6654fddba2a6fe08cfa5eca |
| institution | Kabale University |
| issn | 2412-1908 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Altai State University |
| record_format | Article |
| series | Acta Biologica Sibirica |
| spelling | doaj-art-f9d57e88f6654fddba2a6fe08cfa5eca2025-01-23T08:30:29ZengAltai State UniversityActa Biologica Sibirica2412-19082024-12-01101433–14431433–144310.5281/zenodo.1427988716502Species identification based on the fecal DNA samples of the CaprinaeAbdurakhim E. Kuchboev0Oybek O. Amirov1Makhamadi B. Abramatov2Bakhtiyor H. Ruziev3Mehmonjon Kh. Egamberdiyev4Rokhatoy R. Karimova5Institute of Zoology of the Academy of Sciences of the Republic of Uzbekistan, 232b Bogishamol st., Tashkent, 100053, UzbekistanInstitute of Zoology of the Academy of Sciences of the Republic of Uzbekistan, 232b Bogishamol st., Tashkent, 100053, UzbekistanTermez State University, 43 Barkamol Avlod St., Termez, 190111, UzbekistanKarshi State University, 17 Kuchabag St., Karshi, 180119, UzbekistanNamangan State University, 161 Baburshoh St., Namangan, 160107, UzbekistanInstitute of Zoology of the Academy of Sciences of the Republic of Uzbekistan, 232b Bogishamol st., Tashkent, 100053, UzbekistanFecal analysis is a useful tool for studying the species identity of rare mammals. The possibility of using non-invasive biological materials in molecular genetic studies of rare bovids is shown, using the example of the markhor and Siberian ibex of Uzbekistan. Field work including noninvasive genetic sampling collection was carried out in the study area in spring and autumn 2022-2023 in the Hissar, Surkhan State Reserves and Ugam-Chatkal State National Natural Park and Termez zoo in Uzbekistan. We used species-specific 16S rRNA mitochondrial gene fragments for polymerase chain reaction amplification for species identification. The results of the molecular analysis with the 16S rRNA mitochondrial gene allowed the identification of Capra sibirica, C. falconeri and C. hircus belonging to the subfamily Caprinae using a noninvasive genetic sampling method. This method is quite easy to use, while avoiding direct contact with the animal, which minimizes the degree of impact on the object being studied and does not require significant material and labor costs for researchers. We believe that noninvasive genetic sampling is emerging as one of the most effective and accurate methods for estimating the population size of animals, and we recommend considering this approach for endangered and rare species. The protocol developed could be a valuable tool in the management and conservation of the Capra species occurring on the Uzbekistan.http://journal.asu.ru/biol/article/view/16502capranoninvasive genetic samplingpcrrdna16sspecies identification |
| spellingShingle | Abdurakhim E. Kuchboev Oybek O. Amirov Makhamadi B. Abramatov Bakhtiyor H. Ruziev Mehmonjon Kh. Egamberdiyev Rokhatoy R. Karimova Species identification based on the fecal DNA samples of the Caprinae Acta Biologica Sibirica capra noninvasive genetic sampling pcr rdna 16s species identification |
| title | Species identification based on the fecal DNA samples of the Caprinae |
| title_full | Species identification based on the fecal DNA samples of the Caprinae |
| title_fullStr | Species identification based on the fecal DNA samples of the Caprinae |
| title_full_unstemmed | Species identification based on the fecal DNA samples of the Caprinae |
| title_short | Species identification based on the fecal DNA samples of the Caprinae |
| title_sort | species identification based on the fecal dna samples of the caprinae |
| topic | capra noninvasive genetic sampling pcr rdna 16s species identification |
| url | http://journal.asu.ru/biol/article/view/16502 |
| work_keys_str_mv | AT abdurakhimekuchboev speciesidentificationbasedonthefecaldnasamplesofthecaprinae AT oybekoamirov speciesidentificationbasedonthefecaldnasamplesofthecaprinae AT makhamadibabramatov speciesidentificationbasedonthefecaldnasamplesofthecaprinae AT bakhtiyorhruziev speciesidentificationbasedonthefecaldnasamplesofthecaprinae AT mehmonjonkhegamberdiyev speciesidentificationbasedonthefecaldnasamplesofthecaprinae AT rokhatoyrkarimova speciesidentificationbasedonthefecaldnasamplesofthecaprinae |