Identification of potential miRNA biomarkers for neurobrucellosis diagnosis
BackgroundNeurobrucellosis (NB) is a rare complication of brucellosis that presentis with a variety of clinical symptoms, complicating its diagnosis. This study aimed to analyze the microRNA (miRNA) profile of cerebrospinal fluid (CSF) in NB patients to identify potential biomarkers for NB diagnosis...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2025.1463597/full |
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| author | Meiling Liu Liping Feng Shujun Shi Yuzhou Guo Menghan Lv Zhengyu Li Xintian Wang Hao Yang Zhelin Zhang |
| author_facet | Meiling Liu Liping Feng Shujun Shi Yuzhou Guo Menghan Lv Zhengyu Li Xintian Wang Hao Yang Zhelin Zhang |
| author_sort | Meiling Liu |
| collection | DOAJ |
| description | BackgroundNeurobrucellosis (NB) is a rare complication of brucellosis that presentis with a variety of clinical symptoms, complicating its diagnosis. This study aimed to analyze the microRNA (miRNA) profile of cerebrospinal fluid (CSF) in NB patients to identify potential biomarkers for NB diagnosis.MethodsSmall RNA sequencing was utilized to identify differential expressed miRNAs (DEmiRNAs) in CSF samples from both control and NB patients (in-house cohort). The GSE107554 dataset (validation cohort) from the GEO database was used to validate the expression levels of these DEmiRNAs. Subsequently, LASSO regression analysis was conducted to construct a diagnostic risk score model based on common miRNAs identified in both the in-house and validation cohorts.ResultsThe study identified 51 DEmiRNAs between the control and NB groups, based on the data from the in-house cohort. Among these, four common miRNAs (miR-342-3p, miR-576-5p, miR-15b-5p and miR-499a-5p) were significantly elevated in the infection group compared to controls in both the in-house and validation cohorts. Subsequently, a novel NB diagnostic signature comprising two miRNAs (miR-576-5p and miR-499a-5p) was developed using the LASSO regression analysis. Receiver operating characteristic (ROC) analysis demonstrated the good diagnostic potential of this miRNA signature in both cohorts. RT-qPCR analysis indicated elevated levels of miR-576-5p and miR-499a-5p in CSF samples of NB patients compared to controls. Additionally, RT-qPCR results revealed decreased levels of VAV3 (vav guanine nucleotide exchange factor 3, a target of miR-499a-5p) and IGF1 (insulin like growth factor 1, a target of miR-576-5p) in the CSF samples of NB patients.ConclusionCollectively, miR-576-5p and miR-499a-5p may serve as potential biomarkers for the diagnosis of NB. |
| format | Article |
| id | doaj-art-f9d45c14c7b948c48d75b484f52f4e2f |
| institution | OA Journals |
| issn | 1664-042X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj-art-f9d45c14c7b948c48d75b484f52f4e2f2025-08-20T02:36:53ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2025-06-011610.3389/fphys.2025.14635971463597Identification of potential miRNA biomarkers for neurobrucellosis diagnosisMeiling Liu0Liping Feng1Shujun Shi2Yuzhou Guo3Menghan Lv4Zhengyu Li5Xintian Wang6Hao Yang7Zhelin Zhang8Department of Neurology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, ChinaXing’an League Disease Prevention and Control Center, Ulanhot, ChinaDepartment of Neurology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, ChinaDepartment of Neurology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, ChinaDepartment of Neurology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, ChinaDepartment of Neurology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, ChinaDepartment of Neurology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, ChinaDepartment of Radiation Oncology, Inner Mongolia Cancer Hospital & Affiliated People’s Hospital of Inner Mongolia Medical University, Hohhot, ChinaDepartment of Neurology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, ChinaBackgroundNeurobrucellosis (NB) is a rare complication of brucellosis that presentis with a variety of clinical symptoms, complicating its diagnosis. This study aimed to analyze the microRNA (miRNA) profile of cerebrospinal fluid (CSF) in NB patients to identify potential biomarkers for NB diagnosis.MethodsSmall RNA sequencing was utilized to identify differential expressed miRNAs (DEmiRNAs) in CSF samples from both control and NB patients (in-house cohort). The GSE107554 dataset (validation cohort) from the GEO database was used to validate the expression levels of these DEmiRNAs. Subsequently, LASSO regression analysis was conducted to construct a diagnostic risk score model based on common miRNAs identified in both the in-house and validation cohorts.ResultsThe study identified 51 DEmiRNAs between the control and NB groups, based on the data from the in-house cohort. Among these, four common miRNAs (miR-342-3p, miR-576-5p, miR-15b-5p and miR-499a-5p) were significantly elevated in the infection group compared to controls in both the in-house and validation cohorts. Subsequently, a novel NB diagnostic signature comprising two miRNAs (miR-576-5p and miR-499a-5p) was developed using the LASSO regression analysis. Receiver operating characteristic (ROC) analysis demonstrated the good diagnostic potential of this miRNA signature in both cohorts. RT-qPCR analysis indicated elevated levels of miR-576-5p and miR-499a-5p in CSF samples of NB patients compared to controls. Additionally, RT-qPCR results revealed decreased levels of VAV3 (vav guanine nucleotide exchange factor 3, a target of miR-499a-5p) and IGF1 (insulin like growth factor 1, a target of miR-576-5p) in the CSF samples of NB patients.ConclusionCollectively, miR-576-5p and miR-499a-5p may serve as potential biomarkers for the diagnosis of NB.https://www.frontiersin.org/articles/10.3389/fphys.2025.1463597/fullneurobrucellosismiRNAscerebrospinal fluidsmiR-499a-5pmiR-576-5p |
| spellingShingle | Meiling Liu Liping Feng Shujun Shi Yuzhou Guo Menghan Lv Zhengyu Li Xintian Wang Hao Yang Zhelin Zhang Identification of potential miRNA biomarkers for neurobrucellosis diagnosis Frontiers in Physiology neurobrucellosis miRNAs cerebrospinal fluids miR-499a-5p miR-576-5p |
| title | Identification of potential miRNA biomarkers for neurobrucellosis diagnosis |
| title_full | Identification of potential miRNA biomarkers for neurobrucellosis diagnosis |
| title_fullStr | Identification of potential miRNA biomarkers for neurobrucellosis diagnosis |
| title_full_unstemmed | Identification of potential miRNA biomarkers for neurobrucellosis diagnosis |
| title_short | Identification of potential miRNA biomarkers for neurobrucellosis diagnosis |
| title_sort | identification of potential mirna biomarkers for neurobrucellosis diagnosis |
| topic | neurobrucellosis miRNAs cerebrospinal fluids miR-499a-5p miR-576-5p |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2025.1463597/full |
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