Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E2 and F2α Metabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes
Background. Our aim was to evaluate the protective effect of lipoic acid (LA) on fetal outcome of diabetic mothers. Methods. Diabetes was induced in female rats using streptozotocin and rats were made pregnant. Pregnant control (group 1; n=9; and group 2; n=7) or pregnant diabetic (group 3; n=10; an...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/9354937 |
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| author | Hisham Y. Al-Matubsi Ghaleb A. Oriquat Mahmoud Abu-Samak Othman A. Al Hanbali Maher D. Salim |
| author_facet | Hisham Y. Al-Matubsi Ghaleb A. Oriquat Mahmoud Abu-Samak Othman A. Al Hanbali Maher D. Salim |
| author_sort | Hisham Y. Al-Matubsi |
| collection | DOAJ |
| description | Background. Our aim was to evaluate the protective effect of lipoic acid (LA) on fetal outcome of diabetic mothers. Methods. Diabetes was induced in female rats using streptozotocin and rats were made pregnant. Pregnant control (group 1; n=9; and group 2; n=7) or pregnant diabetic (group 3; n=10; and group 4; n=8) rats were treated daily with either LA (groups 2 and 4) or vehicle (groups 1 and 3) between gestational days 0 and 15. On day 15 of gestation, the fetuses, placentas, and membranes were dissected, examined morphologically, and then homogenized, to measure cyclooxygenase (COX) activities and metabolisms of prostaglandin (PG) E2 (PGEM) and PGF2α (PGFM) levels. The level of total glutathione was measured in the maternal liver and plasma and in all fetuses. Results. Supplementation of diabetic rats with LA was found to significantly (p<0.05) reduce resorption rates in diabetic rats and led to a significant (p<0.05) increase in liver, plasma, and fetuses total glutathione from LA-TD rats as compared to those from V-TD. Decreased levels of PGEM and elevated levels of PGFM in the fetuses, placentas, and membranes were characteristic of experimental diabetic gestation associated with malformation. The levels of PGEM in malformed fetuses from LA-TD mothers was significantly (p<0.05) higher than those in malformed fetuses from V-TD rats. Conclusions. LA treatment did not completely prevent the occurrence of malformations. Thus, other factors may be involved in the pathogenesis of the diabetes-induced congenital malformations. |
| format | Article |
| id | doaj-art-f9c3f7d5598d449eb89e1b3a8c65a90c |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-f9c3f7d5598d449eb89e1b3a8c65a90c2025-02-03T01:27:18ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/93549379354937Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E2 and F2α Metabolites, in the Offspring of Rats with Streptozotocin-Induced DiabetesHisham Y. Al-Matubsi0Ghaleb A. Oriquat1Mahmoud Abu-Samak2Othman A. Al Hanbali3Maher D. Salim4Faculty of Pharmacy, University of Petra, Amman, JordanFaculty of Pharmacy and Medical Sciences, Al-Ahliyya Amman University, Amman, JordanFaculty of Pharmacy, Applied Science University, Amman, JordanFaculty of Pharmacy, Al-Zaytoonah University, Amman, JordanMiddle East University, Amman, JordanBackground. Our aim was to evaluate the protective effect of lipoic acid (LA) on fetal outcome of diabetic mothers. Methods. Diabetes was induced in female rats using streptozotocin and rats were made pregnant. Pregnant control (group 1; n=9; and group 2; n=7) or pregnant diabetic (group 3; n=10; and group 4; n=8) rats were treated daily with either LA (groups 2 and 4) or vehicle (groups 1 and 3) between gestational days 0 and 15. On day 15 of gestation, the fetuses, placentas, and membranes were dissected, examined morphologically, and then homogenized, to measure cyclooxygenase (COX) activities and metabolisms of prostaglandin (PG) E2 (PGEM) and PGF2α (PGFM) levels. The level of total glutathione was measured in the maternal liver and plasma and in all fetuses. Results. Supplementation of diabetic rats with LA was found to significantly (p<0.05) reduce resorption rates in diabetic rats and led to a significant (p<0.05) increase in liver, plasma, and fetuses total glutathione from LA-TD rats as compared to those from V-TD. Decreased levels of PGEM and elevated levels of PGFM in the fetuses, placentas, and membranes were characteristic of experimental diabetic gestation associated with malformation. The levels of PGEM in malformed fetuses from LA-TD mothers was significantly (p<0.05) higher than those in malformed fetuses from V-TD rats. Conclusions. LA treatment did not completely prevent the occurrence of malformations. Thus, other factors may be involved in the pathogenesis of the diabetes-induced congenital malformations.http://dx.doi.org/10.1155/2016/9354937 |
| spellingShingle | Hisham Y. Al-Matubsi Ghaleb A. Oriquat Mahmoud Abu-Samak Othman A. Al Hanbali Maher D. Salim Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E2 and F2α Metabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes Journal of Diabetes Research |
| title | Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E2 and F2α Metabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes |
| title_full | Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E2 and F2α Metabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes |
| title_fullStr | Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E2 and F2α Metabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes |
| title_full_unstemmed | Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E2 and F2α Metabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes |
| title_short | Effects of Lipoic Acid Supplementation on Activities of Cyclooxygenases and Levels of Prostaglandins E2 and F2α Metabolites, in the Offspring of Rats with Streptozotocin-Induced Diabetes |
| title_sort | effects of lipoic acid supplementation on activities of cyclooxygenases and levels of prostaglandins e2 and f2α metabolites in the offspring of rats with streptozotocin induced diabetes |
| url | http://dx.doi.org/10.1155/2016/9354937 |
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