NupR Is Involved in the Control of PlcR: A Pleiotropic Regulator of Extracellular Virulence Factors
NupR is a nucleoside permease regulator belonging to the GntR family, mainly regulating nucleoside transport in <i>Bacillus thuringiensis</i>. A conserved binding site for NupR was found in the promoter region of <i>plcR</i>. This study aimed to investigate the regulation of...
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2025-01-01
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| author | Jiaxin Qin Ziqi Wang Cheng Qian Guohui Ji Yizhuo Zhang Zhanglei Cao Bing Yan Jun Cai |
| author_facet | Jiaxin Qin Ziqi Wang Cheng Qian Guohui Ji Yizhuo Zhang Zhanglei Cao Bing Yan Jun Cai |
| author_sort | Jiaxin Qin |
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| description | NupR is a nucleoside permease regulator belonging to the GntR family, mainly regulating nucleoside transport in <i>Bacillus thuringiensis</i>. A conserved binding site for NupR was found in the promoter region of <i>plcR</i>. This study aimed to investigate the regulation of the virulence regulator PlcR by NupR and its impact on Bt virulence. We demonstrated that NupR can directly repress the expression of <i>plcR</i>. The expression of <i>plcR</i> can be induced by glucose and nucleosides. Glucose impacts the expression of <i>plcR</i> mainly through Spo0A, while the induction effect of nucleosides may be due to the production of ribose through nucleoside catabolism. In addition, NupR regulates the expression of the PlcR regulon, including hemolysin, phospholipase C, <i>papR</i>, and oligopeptide permease, which could result in the culture supernatant of BMB171 being less virulent to sf9 cells compared to the <i>nupR</i> knockout strain. The results combine the nutritional status of cells with virulence to form a regulatory loop, providing new ideas and research foundations for the study of bacterial virulence. |
| format | Article |
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| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-f97bbbb72a7b460d92ccc5b77b795c702025-01-24T13:43:05ZengMDPI AGMicroorganisms2076-26072025-01-0113121210.3390/microorganisms13010212NupR Is Involved in the Control of PlcR: A Pleiotropic Regulator of Extracellular Virulence FactorsJiaxin Qin0Ziqi Wang1Cheng Qian2Guohui Ji3Yizhuo Zhang4Zhanglei Cao5Bing Yan6Jun Cai7Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, ChinaDepartment of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, ChinaDepartment of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, ChinaDepartment of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, ChinaDepartment of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, ChinaDepartment of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, ChinaDepartment of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, ChinaDepartment of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, ChinaNupR is a nucleoside permease regulator belonging to the GntR family, mainly regulating nucleoside transport in <i>Bacillus thuringiensis</i>. A conserved binding site for NupR was found in the promoter region of <i>plcR</i>. This study aimed to investigate the regulation of the virulence regulator PlcR by NupR and its impact on Bt virulence. We demonstrated that NupR can directly repress the expression of <i>plcR</i>. The expression of <i>plcR</i> can be induced by glucose and nucleosides. Glucose impacts the expression of <i>plcR</i> mainly through Spo0A, while the induction effect of nucleosides may be due to the production of ribose through nucleoside catabolism. In addition, NupR regulates the expression of the PlcR regulon, including hemolysin, phospholipase C, <i>papR</i>, and oligopeptide permease, which could result in the culture supernatant of BMB171 being less virulent to sf9 cells compared to the <i>nupR</i> knockout strain. The results combine the nutritional status of cells with virulence to form a regulatory loop, providing new ideas and research foundations for the study of bacterial virulence.https://www.mdpi.com/2076-2607/13/1/212PlcRNupRvirulenceglucose<i>Bacillus thuringiensis</i> |
| spellingShingle | Jiaxin Qin Ziqi Wang Cheng Qian Guohui Ji Yizhuo Zhang Zhanglei Cao Bing Yan Jun Cai NupR Is Involved in the Control of PlcR: A Pleiotropic Regulator of Extracellular Virulence Factors Microorganisms PlcR NupR virulence glucose <i>Bacillus thuringiensis</i> |
| title | NupR Is Involved in the Control of PlcR: A Pleiotropic Regulator of Extracellular Virulence Factors |
| title_full | NupR Is Involved in the Control of PlcR: A Pleiotropic Regulator of Extracellular Virulence Factors |
| title_fullStr | NupR Is Involved in the Control of PlcR: A Pleiotropic Regulator of Extracellular Virulence Factors |
| title_full_unstemmed | NupR Is Involved in the Control of PlcR: A Pleiotropic Regulator of Extracellular Virulence Factors |
| title_short | NupR Is Involved in the Control of PlcR: A Pleiotropic Regulator of Extracellular Virulence Factors |
| title_sort | nupr is involved in the control of plcr a pleiotropic regulator of extracellular virulence factors |
| topic | PlcR NupR virulence glucose <i>Bacillus thuringiensis</i> |
| url | https://www.mdpi.com/2076-2607/13/1/212 |
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