Phytochemical Evaluation of Moth Bean (Vigna aconitifolia L.) Seeds and Their Divergence

In the present study, phytochemical contents of 25 moth bean (Vigna aconitifolia) seed accessions were evaluated. This includes protease inhibitors, phytic acid, radical scavenging activity, and tannins. The studies revealed significant variation in the contents of theses phytochemicals. Presence of...

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Main Authors: Neha Gupta, Nidhi Shrivastava, Pramod Kumar Singh, Sameer S. Bhagyawant
Format: Article
Language:English
Published: Wiley 2016-01-01
Series:Biochemistry Research International
Online Access:http://dx.doi.org/10.1155/2016/3136043
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author Neha Gupta
Nidhi Shrivastava
Pramod Kumar Singh
Sameer S. Bhagyawant
author_facet Neha Gupta
Nidhi Shrivastava
Pramod Kumar Singh
Sameer S. Bhagyawant
author_sort Neha Gupta
collection DOAJ
description In the present study, phytochemical contents of 25 moth bean (Vigna aconitifolia) seed accessions were evaluated. This includes protease inhibitors, phytic acid, radical scavenging activity, and tannins. The studies revealed significant variation in the contents of theses phytochemicals. Presence of photochemical composition was correlated with seed storage proteins like albumin and globulin. Qualitative identification of total seed storage protein abundance across two related moth bean accessions using two-dimensional gel electrophoresis (2D-GE) was performed. Over 20 individual protein fractions were distributed over the gel as a series of spots in two moth bean accessions. Seed proteome accumulated spots of high intensity over a broad range of pI values of 3–10 in a molecular weight range of 11–170 kDa. In both seed accessions maximum protein spots are seen in the pI range of 6–8.
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institution Kabale University
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series Biochemistry Research International
spelling doaj-art-f979884c9ae2464b81cb41e3afa0d19a2025-02-03T06:12:47ZengWileyBiochemistry Research International2090-22472090-22552016-01-01201610.1155/2016/31360433136043Phytochemical Evaluation of Moth Bean (Vigna aconitifolia L.) Seeds and Their DivergenceNeha Gupta0Nidhi Shrivastava1Pramod Kumar Singh2Sameer S. Bhagyawant3School of Studies in Biotechnology, Jiwaji University, Gwalior 474011, IndiaDepartment of Bioscience & Biotechnology, Banasthali University, Banasthali 304022, IndiaSchool of Studies in Biotechnology, Jiwaji University, Gwalior 474011, IndiaSchool of Studies in Biotechnology, Jiwaji University, Gwalior 474011, IndiaIn the present study, phytochemical contents of 25 moth bean (Vigna aconitifolia) seed accessions were evaluated. This includes protease inhibitors, phytic acid, radical scavenging activity, and tannins. The studies revealed significant variation in the contents of theses phytochemicals. Presence of photochemical composition was correlated with seed storage proteins like albumin and globulin. Qualitative identification of total seed storage protein abundance across two related moth bean accessions using two-dimensional gel electrophoresis (2D-GE) was performed. Over 20 individual protein fractions were distributed over the gel as a series of spots in two moth bean accessions. Seed proteome accumulated spots of high intensity over a broad range of pI values of 3–10 in a molecular weight range of 11–170 kDa. In both seed accessions maximum protein spots are seen in the pI range of 6–8.http://dx.doi.org/10.1155/2016/3136043
spellingShingle Neha Gupta
Nidhi Shrivastava
Pramod Kumar Singh
Sameer S. Bhagyawant
Phytochemical Evaluation of Moth Bean (Vigna aconitifolia L.) Seeds and Their Divergence
Biochemistry Research International
title Phytochemical Evaluation of Moth Bean (Vigna aconitifolia L.) Seeds and Their Divergence
title_full Phytochemical Evaluation of Moth Bean (Vigna aconitifolia L.) Seeds and Their Divergence
title_fullStr Phytochemical Evaluation of Moth Bean (Vigna aconitifolia L.) Seeds and Their Divergence
title_full_unstemmed Phytochemical Evaluation of Moth Bean (Vigna aconitifolia L.) Seeds and Their Divergence
title_short Phytochemical Evaluation of Moth Bean (Vigna aconitifolia L.) Seeds and Their Divergence
title_sort phytochemical evaluation of moth bean vigna aconitifolia l seeds and their divergence
url http://dx.doi.org/10.1155/2016/3136043
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AT pramodkumarsingh phytochemicalevaluationofmothbeanvignaaconitifolialseedsandtheirdivergence
AT sameersbhagyawant phytochemicalevaluationofmothbeanvignaaconitifolialseedsandtheirdivergence