Therapeutic Effects of the Superoxide Dismutase Mimetic Compound Me2DO2A on Experimental Articular Pain in Rats
Superoxide anion () is overproduced in joint inflammation, rheumatoid arthritis, and osteoarthritis. Increased production leads to tissue damage, articular degeneration, and pain. In these conditions, the physiological defense against , superoxide dismutases (SOD) are decreased. The complex MnL4 i...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/905360 |
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| author | Lorenzo Di Cesare Mannelli Daniele Bani Andrea Bencini Maria Luisa Brandi Laura Calosi Miriam Cantore Anna Maria Carossino Carla Ghelardini Barbara Valtancoli Paola Failli |
| author_facet | Lorenzo Di Cesare Mannelli Daniele Bani Andrea Bencini Maria Luisa Brandi Laura Calosi Miriam Cantore Anna Maria Carossino Carla Ghelardini Barbara Valtancoli Paola Failli |
| author_sort | Lorenzo Di Cesare Mannelli |
| collection | DOAJ |
| description | Superoxide anion () is overproduced in joint inflammation, rheumatoid arthritis, and osteoarthritis. Increased production leads to tissue damage, articular degeneration, and pain. In these conditions, the physiological defense against , superoxide dismutases (SOD) are decreased. The complex MnL4 is a potent SOD mimetic, and in this study it was tested in inflammatory and osteoarticular rat pain models. In vivo protocols were approved by the animal Ethical Committee of the University of Florence. Pain was measured by paw pressure and hind limb weight bearing alterations tests.
MnL4 (15 mg kg−1) acutely administered, significantly reduced pain induced by carrageenan, complete Freund’s adjuvant (CFA), and sodium monoiodoacetate (MIA). In CFA and MIA protocols, it ameliorated the alteration of postural equilibrium. When administered by osmotic pump in the MIA osteoarthritis, MnL4 reduced pain, articular derangement, plasma TNF alpha levels, and protein carbonylation. The scaffold ring was ineffective. MnL4 (10−7 M) prevented the lipid peroxidation of isolated human chondrocytes when was produced by RAW 264.7. MnL4 behaves as a potent pain reliever in acute inflammatory and chronic articular pain, being its efficacy related to antioxidant property. Therefore MnL4 appears as a novel protective compound potentially suitable for the treatment of joint diseases. |
| format | Article |
| id | doaj-art-f952786a7a5a44dd9fd70f9d712f2a86 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-f952786a7a5a44dd9fd70f9d712f2a862025-02-03T05:51:52ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/905360905360Therapeutic Effects of the Superoxide Dismutase Mimetic Compound Me2DO2A on Experimental Articular Pain in RatsLorenzo Di Cesare Mannelli0Daniele Bani1Andrea Bencini2Maria Luisa Brandi3Laura Calosi4Miriam Cantore5Anna Maria Carossino6Carla Ghelardini7Barbara Valtancoli8Paola Failli9Department of Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Florence, ItalyDepartment of Anatomy, Histology and Forensic Medicine, University of Florence, Viale Pieraccini 6, 50139 Florence, ItalyDepartment of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, ItalyDepartment of Internal Medicine, University of Florence, Viale Pieraccini 6, 50139 Florence, ItalyDepartment of Anatomy, Histology and Forensic Medicine, University of Florence, Viale Pieraccini 6, 50139 Florence, ItalyDepartment of Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Florence, ItalyDepartment of Internal Medicine, University of Florence, Viale Pieraccini 6, 50139 Florence, ItalyDepartment of Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Florence, ItalyDepartment of Chemistry, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, ItalyDepartment of Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Florence, ItalySuperoxide anion () is overproduced in joint inflammation, rheumatoid arthritis, and osteoarthritis. Increased production leads to tissue damage, articular degeneration, and pain. In these conditions, the physiological defense against , superoxide dismutases (SOD) are decreased. The complex MnL4 is a potent SOD mimetic, and in this study it was tested in inflammatory and osteoarticular rat pain models. In vivo protocols were approved by the animal Ethical Committee of the University of Florence. Pain was measured by paw pressure and hind limb weight bearing alterations tests. MnL4 (15 mg kg−1) acutely administered, significantly reduced pain induced by carrageenan, complete Freund’s adjuvant (CFA), and sodium monoiodoacetate (MIA). In CFA and MIA protocols, it ameliorated the alteration of postural equilibrium. When administered by osmotic pump in the MIA osteoarthritis, MnL4 reduced pain, articular derangement, plasma TNF alpha levels, and protein carbonylation. The scaffold ring was ineffective. MnL4 (10−7 M) prevented the lipid peroxidation of isolated human chondrocytes when was produced by RAW 264.7. MnL4 behaves as a potent pain reliever in acute inflammatory and chronic articular pain, being its efficacy related to antioxidant property. Therefore MnL4 appears as a novel protective compound potentially suitable for the treatment of joint diseases.http://dx.doi.org/10.1155/2013/905360 |
| spellingShingle | Lorenzo Di Cesare Mannelli Daniele Bani Andrea Bencini Maria Luisa Brandi Laura Calosi Miriam Cantore Anna Maria Carossino Carla Ghelardini Barbara Valtancoli Paola Failli Therapeutic Effects of the Superoxide Dismutase Mimetic Compound Me2DO2A on Experimental Articular Pain in Rats Mediators of Inflammation |
| title | Therapeutic Effects of the Superoxide Dismutase Mimetic Compound Me2DO2A on Experimental Articular Pain in Rats |
| title_full | Therapeutic Effects of the Superoxide Dismutase Mimetic Compound Me2DO2A on Experimental Articular Pain in Rats |
| title_fullStr | Therapeutic Effects of the Superoxide Dismutase Mimetic Compound Me2DO2A on Experimental Articular Pain in Rats |
| title_full_unstemmed | Therapeutic Effects of the Superoxide Dismutase Mimetic Compound Me2DO2A on Experimental Articular Pain in Rats |
| title_short | Therapeutic Effects of the Superoxide Dismutase Mimetic Compound Me2DO2A on Experimental Articular Pain in Rats |
| title_sort | therapeutic effects of the superoxide dismutase mimetic compound me2do2a on experimental articular pain in rats |
| url | http://dx.doi.org/10.1155/2013/905360 |
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