Quality Attributes, Structural Characteristics, and Functional Properties of Brewer’s Spent Grain Protein Concentrates as Affected by Alkaline and Pulsed Electric Field-Assisted Extraction
The rising protein demand has driven intensified research into alternative protein sources and extraction technologies. Brewer’s spent grain (BSG), which is rich in protein, remains mostly underutilized. This study aimed to optimize BSG protein extraction conditions using pulsed electric field (PEF)...
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MDPI AG
2025-04-01
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| author | Parichat Paksin Pipat Tangjaidee Wannaporn Klangpetch Kridsada Unban Tabkrich Khumsap Warinporn Klunklin Artit Yawootti Kittisak Jantanasakulwong Pornchai Rachtanapun Suphat Phongthai |
| author_facet | Parichat Paksin Pipat Tangjaidee Wannaporn Klangpetch Kridsada Unban Tabkrich Khumsap Warinporn Klunklin Artit Yawootti Kittisak Jantanasakulwong Pornchai Rachtanapun Suphat Phongthai |
| author_sort | Parichat Paksin |
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| description | The rising protein demand has driven intensified research into alternative protein sources and extraction technologies. Brewer’s spent grain (BSG), which is rich in protein, remains mostly underutilized. This study aimed to optimize BSG protein extraction conditions using pulsed electric field (PEF) by assessing the influence of pulse numbers (5000–9000), electric field strength (8–10 kV/cm), and frequency (8–10 Hz) on protein recovery and purity. The optimized conditions (5386 pulses, 10 kV/cm field strength, and 10 Hz frequency) provided a higher extraction yield with a significant improvement of approximately 90% (<i>p</i> < 0.05). Essential amino acids in proteins extracted via PEF were significantly increased (60,864.84 mg/100 g), particularly phenylalanine, threonine, and valine; furthermore, amino acid score (AAS) and protein digestibility-corrected amino acid score (PDCAAS) were found to be superior to those of protein obtained through alkaline extraction. The PEF treatment resulted in the modification of the secondary structures of proteins from α-helices and β-turns to β-sheets, as well as the enhancement of the hydrophobic−hydrophilic amino acid balance. The functional properties of the proteins, particularly their foaming properties and solubility, were significantly affected by PEF (<i>p</i> < 0.05). In conclusion, PEF-assisted extraction produces high-quality BSG protein concentrates efficiently while rendering the process environmentally sustainable. |
| format | Article |
| id | doaj-art-f9328c1102b64cb38f987dcb44b4d69b |
| institution | OA Journals |
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| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj-art-f9328c1102b64cb38f987dcb44b4d69b2025-08-20T02:24:47ZengMDPI AGFoods2304-81582025-04-01149151510.3390/foods14091515Quality Attributes, Structural Characteristics, and Functional Properties of Brewer’s Spent Grain Protein Concentrates as Affected by Alkaline and Pulsed Electric Field-Assisted ExtractionParichat Paksin0Pipat Tangjaidee1Wannaporn Klangpetch2Kridsada Unban3Tabkrich Khumsap4Warinporn Klunklin5Artit Yawootti6Kittisak Jantanasakulwong7Pornchai Rachtanapun8Suphat Phongthai9Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandFaculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandFaculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandFaculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandFaculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandFaculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandDepartment of Electrical Engineering, Faculty of Engineering, Rajamangala University of Technology Lanna, Chiang Mai 50300, ThailandFaculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandFaculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandFaculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandThe rising protein demand has driven intensified research into alternative protein sources and extraction technologies. Brewer’s spent grain (BSG), which is rich in protein, remains mostly underutilized. This study aimed to optimize BSG protein extraction conditions using pulsed electric field (PEF) by assessing the influence of pulse numbers (5000–9000), electric field strength (8–10 kV/cm), and frequency (8–10 Hz) on protein recovery and purity. The optimized conditions (5386 pulses, 10 kV/cm field strength, and 10 Hz frequency) provided a higher extraction yield with a significant improvement of approximately 90% (<i>p</i> < 0.05). Essential amino acids in proteins extracted via PEF were significantly increased (60,864.84 mg/100 g), particularly phenylalanine, threonine, and valine; furthermore, amino acid score (AAS) and protein digestibility-corrected amino acid score (PDCAAS) were found to be superior to those of protein obtained through alkaline extraction. The PEF treatment resulted in the modification of the secondary structures of proteins from α-helices and β-turns to β-sheets, as well as the enhancement of the hydrophobic−hydrophilic amino acid balance. The functional properties of the proteins, particularly their foaming properties and solubility, were significantly affected by PEF (<i>p</i> < 0.05). In conclusion, PEF-assisted extraction produces high-quality BSG protein concentrates efficiently while rendering the process environmentally sustainable.https://www.mdpi.com/2304-8158/14/9/1515pulsed electric fieldbrewer’s spent grainfunctional propertiesalternative protein |
| spellingShingle | Parichat Paksin Pipat Tangjaidee Wannaporn Klangpetch Kridsada Unban Tabkrich Khumsap Warinporn Klunklin Artit Yawootti Kittisak Jantanasakulwong Pornchai Rachtanapun Suphat Phongthai Quality Attributes, Structural Characteristics, and Functional Properties of Brewer’s Spent Grain Protein Concentrates as Affected by Alkaline and Pulsed Electric Field-Assisted Extraction Foods pulsed electric field brewer’s spent grain functional properties alternative protein |
| title | Quality Attributes, Structural Characteristics, and Functional Properties of Brewer’s Spent Grain Protein Concentrates as Affected by Alkaline and Pulsed Electric Field-Assisted Extraction |
| title_full | Quality Attributes, Structural Characteristics, and Functional Properties of Brewer’s Spent Grain Protein Concentrates as Affected by Alkaline and Pulsed Electric Field-Assisted Extraction |
| title_fullStr | Quality Attributes, Structural Characteristics, and Functional Properties of Brewer’s Spent Grain Protein Concentrates as Affected by Alkaline and Pulsed Electric Field-Assisted Extraction |
| title_full_unstemmed | Quality Attributes, Structural Characteristics, and Functional Properties of Brewer’s Spent Grain Protein Concentrates as Affected by Alkaline and Pulsed Electric Field-Assisted Extraction |
| title_short | Quality Attributes, Structural Characteristics, and Functional Properties of Brewer’s Spent Grain Protein Concentrates as Affected by Alkaline and Pulsed Electric Field-Assisted Extraction |
| title_sort | quality attributes structural characteristics and functional properties of brewer s spent grain protein concentrates as affected by alkaline and pulsed electric field assisted extraction |
| topic | pulsed electric field brewer’s spent grain functional properties alternative protein |
| url | https://www.mdpi.com/2304-8158/14/9/1515 |
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