A Novel Role of Globular Adiponectin in Treatment with HFD/STZ Induced T2DM Combined with NAFLD Rats
Aims. To evaluate the effects of globular adiponectin (gAd) on treatment of type 2 diabetic rats combined with NAFLD. Materials and Methods. Twenty-one male wistar rats were fed with normal diet (7 rats) or high fat diet (HFD) (14 rats) for 4 weeks, and then HFD-fed rats were injected with streptozo...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/230835 |
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| author | Hong Ma Guo-Ping You Xuan-Pu Zhang Xiang-Jiu Yang Hua-Dong Lu Yan-Ling Huang Wen-Qiang Zhang |
| author_facet | Hong Ma Guo-Ping You Xuan-Pu Zhang Xiang-Jiu Yang Hua-Dong Lu Yan-Ling Huang Wen-Qiang Zhang |
| author_sort | Hong Ma |
| collection | DOAJ |
| description | Aims. To evaluate the effects of globular adiponectin (gAd) on treatment of type 2 diabetic rats combined with NAFLD. Materials and Methods. Twenty-one male wistar rats were fed with normal diet (7 rats) or high fat diet (HFD) (14 rats) for 4 weeks, and then HFD-fed rats were injected with streptozotocin (STZ) to induce type 2 diabetes mellitus (T2DM). Half of T2DM rats were randomly injected with gAd intraperitoneally for 7 days. The expressions of adiponectin receptors (adipoR1/R2) in liver and skeletal muscle tissues were detected through western blotting or RT-qPCR, respectively. Results. Globular adiponectin alleviated the hepatic steatosis and increased insulin secretion. In liver, both the protein and mRNA expressions of adipoR2 in T2DM group decreased (P<0.05, resp.) in contrast to NC group and increased (P<0.05 and P<0.001, resp.) after gAd treatment. But the protein and mRNA expressions of adipoR1 increased (P<0.05, resp.) in T2DM group and no change was found in the gAd-treated group. In skeletal muscle, the protein and mRNA expressions of adipoR1 and adipoR2 were upregulated in T2DM group and were downregulated after gAd treatment. Conclusions. Globular adiponectin could ameliorate the hepatic steatosis and vary the expressions of adiponectin receptors in liver and skeletal muscle by stimulating insulin secretion. |
| format | Article |
| id | doaj-art-f9195c38003b4c469642c25017611e07 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-f9195c38003b4c469642c25017611e072025-02-03T01:21:17ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/230835230835A Novel Role of Globular Adiponectin in Treatment with HFD/STZ Induced T2DM Combined with NAFLD RatsHong Ma0Guo-Ping You1Xuan-Pu Zhang2Xiang-Jiu Yang3Hua-Dong Lu4Yan-Ling Huang5Wen-Qiang Zhang6Department of Endocrinology, Zhongshan Hospital Xiamen University, 209 Hubin South Road, Xiamen, Fujian 361004, ChinaDepartment of Endocrinology, Zhongshan Hospital Xiamen University, 209 Hubin South Road, Xiamen, Fujian 361004, ChinaDepartment of Endocrinology, Zhongshan Hospital Xiamen University, 209 Hubin South Road, Xiamen, Fujian 361004, ChinaDepartment of Endocrinology, Zhongshan Hospital Xiamen University, 209 Hubin South Road, Xiamen, Fujian 361004, ChinaDepartment of Pathology, Zhongshan Hospital Xiamen University, Xiamen, Fujian 361004, ChinaDepartment of Endocrinology, Zhongshan Hospital Xiamen University, 209 Hubin South Road, Xiamen, Fujian 361004, ChinaDepartment of Endocrinology, Zhongshan Hospital Xiamen University, 209 Hubin South Road, Xiamen, Fujian 361004, ChinaAims. To evaluate the effects of globular adiponectin (gAd) on treatment of type 2 diabetic rats combined with NAFLD. Materials and Methods. Twenty-one male wistar rats were fed with normal diet (7 rats) or high fat diet (HFD) (14 rats) for 4 weeks, and then HFD-fed rats were injected with streptozotocin (STZ) to induce type 2 diabetes mellitus (T2DM). Half of T2DM rats were randomly injected with gAd intraperitoneally for 7 days. The expressions of adiponectin receptors (adipoR1/R2) in liver and skeletal muscle tissues were detected through western blotting or RT-qPCR, respectively. Results. Globular adiponectin alleviated the hepatic steatosis and increased insulin secretion. In liver, both the protein and mRNA expressions of adipoR2 in T2DM group decreased (P<0.05, resp.) in contrast to NC group and increased (P<0.05 and P<0.001, resp.) after gAd treatment. But the protein and mRNA expressions of adipoR1 increased (P<0.05, resp.) in T2DM group and no change was found in the gAd-treated group. In skeletal muscle, the protein and mRNA expressions of adipoR1 and adipoR2 were upregulated in T2DM group and were downregulated after gAd treatment. Conclusions. Globular adiponectin could ameliorate the hepatic steatosis and vary the expressions of adiponectin receptors in liver and skeletal muscle by stimulating insulin secretion.http://dx.doi.org/10.1155/2014/230835 |
| spellingShingle | Hong Ma Guo-Ping You Xuan-Pu Zhang Xiang-Jiu Yang Hua-Dong Lu Yan-Ling Huang Wen-Qiang Zhang A Novel Role of Globular Adiponectin in Treatment with HFD/STZ Induced T2DM Combined with NAFLD Rats The Scientific World Journal |
| title | A Novel Role of Globular Adiponectin in Treatment with HFD/STZ Induced T2DM Combined with NAFLD Rats |
| title_full | A Novel Role of Globular Adiponectin in Treatment with HFD/STZ Induced T2DM Combined with NAFLD Rats |
| title_fullStr | A Novel Role of Globular Adiponectin in Treatment with HFD/STZ Induced T2DM Combined with NAFLD Rats |
| title_full_unstemmed | A Novel Role of Globular Adiponectin in Treatment with HFD/STZ Induced T2DM Combined with NAFLD Rats |
| title_short | A Novel Role of Globular Adiponectin in Treatment with HFD/STZ Induced T2DM Combined with NAFLD Rats |
| title_sort | novel role of globular adiponectin in treatment with hfd stz induced t2dm combined with nafld rats |
| url | http://dx.doi.org/10.1155/2014/230835 |
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