Salivary Metabolomics Fingerprint of Chronic Apical Abscess with Sinus Tract: A Pilot Study
Chronic apical abscess (CAA) is a lesion of apical periodontitis mostly characterized by areas of liquefactive necrosis with disintegrating polymorphonuclear neutrophils surrounded by macrophages. Its presence leads to local bacterial infection, systemic inflammatory response, pain, and swelling. Th...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2019/3162063 |
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| author | Noemi Montis Elisabetta Cotti Antonio Noto Claudia Fattuoni Luigi Barberini |
| author_facet | Noemi Montis Elisabetta Cotti Antonio Noto Claudia Fattuoni Luigi Barberini |
| author_sort | Noemi Montis |
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| description | Chronic apical abscess (CAA) is a lesion of apical periodontitis mostly characterized by areas of liquefactive necrosis with disintegrating polymorphonuclear neutrophils surrounded by macrophages. Its presence leads to local bacterial infection, systemic inflammatory response, pain, and swelling. The use of a novel approach for the study of CAA, such as metabolomics, seems to be important since it has proved to be a powerful tool for biomarkers discovery which could give novel molecular insight on CAA. So, the aim of this study was to verify the possibility to identify the metabolic fingerprint of CAA through the analysis of saliva samples. Nineteen patients were selected for this study: eleven patients affected by CAA with a sinus tract constituted the study group whereas eight patients without clinical and radiographic signs of CAA formed the healthy control group. Saliva samples were collected from each subject and immediately frozen at −80°C. Metabolomic profiles were obtained using a gas chromatography/mass spectrometry instrument. Subsequently, in order to compare the two groups, a multivariate statistical model was built that resulted to be statistically significant. The class of metabolites characterizing the CAA patients was closely related to the bacterial catabolism, tissue necrosis, and presence of a sinus tract. These preliminary results, for the first time, indicate that saliva samples analyzed by means of GC/MS metabolomics may be useful for identifying the presence of CAA, leading to new insights into this disease. |
| format | Article |
| id | doaj-art-f8e96ed098644721b2f54b26962d91e1 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-f8e96ed098644721b2f54b26962d91e12025-02-03T01:03:21ZengWileyThe Scientific World Journal2356-61401537-744X2019-01-01201910.1155/2019/31620633162063Salivary Metabolomics Fingerprint of Chronic Apical Abscess with Sinus Tract: A Pilot StudyNoemi Montis0Elisabetta Cotti1Antonio Noto2Claudia Fattuoni3Luigi Barberini4Department of Conservative Dentistry and Endodontics, University of Cagliari, Cagliari, ItalyDepartment of Conservative Dentistry and Endodontics, University of Cagliari, Cagliari, ItalyDepartment of Medical Sciences and Public Health, University of Cagliari, Cagliari, ItalyDepartment of Chemical and Geological Sciences, University of Cagliari, Cagliari, ItalyDepartment of Medical Sciences and Public Health, University of Cagliari, Cagliari, ItalyChronic apical abscess (CAA) is a lesion of apical periodontitis mostly characterized by areas of liquefactive necrosis with disintegrating polymorphonuclear neutrophils surrounded by macrophages. Its presence leads to local bacterial infection, systemic inflammatory response, pain, and swelling. The use of a novel approach for the study of CAA, such as metabolomics, seems to be important since it has proved to be a powerful tool for biomarkers discovery which could give novel molecular insight on CAA. So, the aim of this study was to verify the possibility to identify the metabolic fingerprint of CAA through the analysis of saliva samples. Nineteen patients were selected for this study: eleven patients affected by CAA with a sinus tract constituted the study group whereas eight patients without clinical and radiographic signs of CAA formed the healthy control group. Saliva samples were collected from each subject and immediately frozen at −80°C. Metabolomic profiles were obtained using a gas chromatography/mass spectrometry instrument. Subsequently, in order to compare the two groups, a multivariate statistical model was built that resulted to be statistically significant. The class of metabolites characterizing the CAA patients was closely related to the bacterial catabolism, tissue necrosis, and presence of a sinus tract. These preliminary results, for the first time, indicate that saliva samples analyzed by means of GC/MS metabolomics may be useful for identifying the presence of CAA, leading to new insights into this disease.http://dx.doi.org/10.1155/2019/3162063 |
| spellingShingle | Noemi Montis Elisabetta Cotti Antonio Noto Claudia Fattuoni Luigi Barberini Salivary Metabolomics Fingerprint of Chronic Apical Abscess with Sinus Tract: A Pilot Study The Scientific World Journal |
| title | Salivary Metabolomics Fingerprint of Chronic Apical Abscess with Sinus Tract: A Pilot Study |
| title_full | Salivary Metabolomics Fingerprint of Chronic Apical Abscess with Sinus Tract: A Pilot Study |
| title_fullStr | Salivary Metabolomics Fingerprint of Chronic Apical Abscess with Sinus Tract: A Pilot Study |
| title_full_unstemmed | Salivary Metabolomics Fingerprint of Chronic Apical Abscess with Sinus Tract: A Pilot Study |
| title_short | Salivary Metabolomics Fingerprint of Chronic Apical Abscess with Sinus Tract: A Pilot Study |
| title_sort | salivary metabolomics fingerprint of chronic apical abscess with sinus tract a pilot study |
| url | http://dx.doi.org/10.1155/2019/3162063 |
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