Bioactivity assessment of peptides derived from salted jellyfish (Rhopilema hispidum) byproducts.
The identification of multifunctional peptides derived from marine byproducts represents a significant challenge in the field. In Thailand, the fisheries industry exports salted jellyfish, which results in low-value byproducts primarily employed for animal feed. Previous studies have indicated the b...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0318781 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The identification of multifunctional peptides derived from marine byproducts represents a significant challenge in the field. In Thailand, the fisheries industry exports salted jellyfish, which results in low-value byproducts primarily employed for animal feed. Previous studies have indicated the bioactivities of jellyfish protein hydrolysates from Lobonema smitthii; however, the multifunctional properties of Rhopilema hispidum remain largely unexplored. This research aimed to characterize synthetic bioactive peptides sourced from the byproducts of salted jellyfish (R. hispidum), with a specific emphasis on their antioxidant, angiotensin-I-converting enzyme (ACE) inhibitory, and anti-inflammatory activities. The hydrolysate obtained from the umbrella portion, subjected to pepsin treatment at a 3:20 enzyme-to-substrate ratio for 48 h at 37 °C, demonstrated the highest levels of antioxidant activity (DPPH = 1.85 ± 0.05 mM TE/mg protein, ABTS = 7.28 ± 0.03 mM TE/mg protein, FRAP = 3.04 ± 0.12 mM FeSO4/mg protein). Following purification, 18 novel peptides exhibiting high antioxidant scores (FRS+CHEL > 0.48) were identified and synthesized. Notably, the peptide MVVACVLPEA exhibited significant antioxidant (DPPH = 56.07 mM TE/mg protein), ACE inhibitory (91.69%), and anti-inflammatory activities (NO release = 34.59 µ M) without cytotoxic effects, although it is important to note that two other peptides did demonstrate cytotoxicity. This investigation reports a total of 16 synthesized peptides that possess triple functional activities-antioxidant, ACE inhibitory, and anti-inflammatory-without cytotoxicity, thus highlighting their potential applications in health-related fields. |
|---|---|
| ISSN: | 1932-6203 |