Continuous Consumption of Reused Palm Oil Induced Hepatic Injury, Depletion of Glutathione Stores, and Modulation of Cytochrome P450 Profiles in Mice
Cooking oil deteriorates with repeated thermal exposure, resulting in appearance change and formation of oxygen radicals. Consumption of the deteriorated oil causes oxidative stress related to lipid metabolism. This study evaluated the effects of palm ( Elaeis guineensis Jacq.) oil on hepatic histo...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2019-01-01
|
| Series: | Polish Journal of Food and Nutrition Sciences |
| Subjects: | |
| Online Access: | http://www.journalssystem.com/pjfns/,101581,0,2.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832572246729687040 |
|---|---|
| author | Waranya Chatuphonprasert Yollada Sriset Kanokwan Jarukamjorn |
| author_facet | Waranya Chatuphonprasert Yollada Sriset Kanokwan Jarukamjorn |
| author_sort | Waranya Chatuphonprasert |
| collection | DOAJ |
| description | Cooking oil deteriorates with repeated thermal exposure, resulting in appearance change and formation of oxygen radicals. Consumption of the deteriorated oil causes oxidative stress related to lipid metabolism. This study evaluated the effects of palm ( Elaeis guineensis Jacq.) oil on hepatic histology, redox status, and cytochrome P450 (CYP) profiles. Adult female mice were orally given purified water (control), fresh, or reused palm oil (4.5 g/kg/day) daily for 16, 24, and 36 weeks. The livers were then collected for histological examination and for the evaluation of the redox system and CYP expression profiles. Treatment with fresh oil for 36 weeks resulted in some pyknosis and karyorrhexis in hepatic tissues, while reused oil resulted in more injuries to the nuclei with hepatic fat accumulation from week 24 onwards. Depletion in reduced glutathione (GSH) stores, with a significant decrease in the GSH/GSSG ratio, was observed with the reused oil but not with the fresh oil. The expression profiles of drug-metabolizing CYPs were significantly modulated; Cyp2c29, Cyp3a11 , and Cyp3a13 were suppressed by both fresh and reused oil, while only the reused oil elevated Cyp2e1 . The expression of Cyp4a10 and Cyp4a14 , the key enzymes in lipid metabolism, were expectedly up-regulated by both. These findings suggest reused oil has a deleterious effect on hepatic ultrastructure, induces an imbalance of redox state, and causes Cyp2e1 activation-associated oxidative stress. It is therefore recommended that fresh rather than reused palm oil be used for cooking, and large-scale or long-term consumption be avoided to reduce the risk of liver damage and drug-interactions. |
| format | Article |
| id | doaj-art-f86e2e0c5c3c45abb63bfd253f75e939 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-f86e2e0c5c3c45abb63bfd253f75e9392025-02-02T11:18:42ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072019-01-01691536110.31883/pjfns-2019-0009101581Continuous Consumption of Reused Palm Oil Induced Hepatic Injury, Depletion of Glutathione Stores, and Modulation of Cytochrome P450 Profiles in MiceWaranya Chatuphonprasert0Yollada Sriset1Kanokwan Jarukamjorn2Faculty of Medicine, Mahasarakham University, Mahasarakham, 44000 ThailandResearch Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Mitrapaab Road, Khon Kaen, 40002,ThailandResearch Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Mitrapaab Road, Khon Kaen, 40002,ThailandCooking oil deteriorates with repeated thermal exposure, resulting in appearance change and formation of oxygen radicals. Consumption of the deteriorated oil causes oxidative stress related to lipid metabolism. This study evaluated the effects of palm ( Elaeis guineensis Jacq.) oil on hepatic histology, redox status, and cytochrome P450 (CYP) profiles. Adult female mice were orally given purified water (control), fresh, or reused palm oil (4.5 g/kg/day) daily for 16, 24, and 36 weeks. The livers were then collected for histological examination and for the evaluation of the redox system and CYP expression profiles. Treatment with fresh oil for 36 weeks resulted in some pyknosis and karyorrhexis in hepatic tissues, while reused oil resulted in more injuries to the nuclei with hepatic fat accumulation from week 24 onwards. Depletion in reduced glutathione (GSH) stores, with a significant decrease in the GSH/GSSG ratio, was observed with the reused oil but not with the fresh oil. The expression profiles of drug-metabolizing CYPs were significantly modulated; Cyp2c29, Cyp3a11 , and Cyp3a13 were suppressed by both fresh and reused oil, while only the reused oil elevated Cyp2e1 . The expression of Cyp4a10 and Cyp4a14 , the key enzymes in lipid metabolism, were expectedly up-regulated by both. These findings suggest reused oil has a deleterious effect on hepatic ultrastructure, induces an imbalance of redox state, and causes Cyp2e1 activation-associated oxidative stress. It is therefore recommended that fresh rather than reused palm oil be used for cooking, and large-scale or long-term consumption be avoided to reduce the risk of liver damage and drug-interactions.http://www.journalssystem.com/pjfns/,101581,0,2.htmlcytochrome p450glutathione storesoxidative stresshistologyliverreused palm oil |
| spellingShingle | Waranya Chatuphonprasert Yollada Sriset Kanokwan Jarukamjorn Continuous Consumption of Reused Palm Oil Induced Hepatic Injury, Depletion of Glutathione Stores, and Modulation of Cytochrome P450 Profiles in Mice Polish Journal of Food and Nutrition Sciences cytochrome p450 glutathione stores oxidative stress histology liver reused palm oil |
| title | Continuous Consumption of Reused Palm Oil Induced Hepatic Injury, Depletion of Glutathione Stores, and Modulation of Cytochrome P450 Profiles in Mice |
| title_full | Continuous Consumption of Reused Palm Oil Induced Hepatic Injury, Depletion of Glutathione Stores, and Modulation of Cytochrome P450 Profiles in Mice |
| title_fullStr | Continuous Consumption of Reused Palm Oil Induced Hepatic Injury, Depletion of Glutathione Stores, and Modulation of Cytochrome P450 Profiles in Mice |
| title_full_unstemmed | Continuous Consumption of Reused Palm Oil Induced Hepatic Injury, Depletion of Glutathione Stores, and Modulation of Cytochrome P450 Profiles in Mice |
| title_short | Continuous Consumption of Reused Palm Oil Induced Hepatic Injury, Depletion of Glutathione Stores, and Modulation of Cytochrome P450 Profiles in Mice |
| title_sort | continuous consumption of reused palm oil induced hepatic injury depletion of glutathione stores and modulation of cytochrome p450 profiles in mice |
| topic | cytochrome p450 glutathione stores oxidative stress histology liver reused palm oil |
| url | http://www.journalssystem.com/pjfns/,101581,0,2.html |
| work_keys_str_mv | AT waranyachatuphonprasert continuousconsumptionofreusedpalmoilinducedhepaticinjurydepletionofglutathionestoresandmodulationofcytochromep450profilesinmice AT yolladasriset continuousconsumptionofreusedpalmoilinducedhepaticinjurydepletionofglutathionestoresandmodulationofcytochromep450profilesinmice AT kanokwanjarukamjorn continuousconsumptionofreusedpalmoilinducedhepaticinjurydepletionofglutathionestoresandmodulationofcytochromep450profilesinmice |