Expression of SET Protein in the Ovaries of Patients with Polycystic Ovary Syndrome
Background. We previously found that expression of SET gene was up-regulated in polycystic ovaries by using microarray. It suggested that SET may be an attractive candidate regulator involved in the pathophysiology of polycystic ovary syndrome (PCOS). In this study, expression and cellular localizat...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2013/367956 |
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| _version_ | 1832559459622191104 |
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| author | Xu Boqun Dai Xiaonan Cui YuGui Gao Lingling Dai Xue Chao Gao Diao Feiyang Liu Jiayin Li Gao Mei Li Yuan Zhang Xiang Ma |
| author_facet | Xu Boqun Dai Xiaonan Cui YuGui Gao Lingling Dai Xue Chao Gao Diao Feiyang Liu Jiayin Li Gao Mei Li Yuan Zhang Xiang Ma |
| author_sort | Xu Boqun |
| collection | DOAJ |
| description | Background. We previously found that expression of SET gene was up-regulated in polycystic ovaries by using microarray. It suggested that SET may be an attractive candidate regulator involved in the pathophysiology of polycystic ovary syndrome (PCOS). In this study, expression and cellular localization of SET protein were investigated in human polycystic and normal ovaries. Method. Ovarian tissues, six normal ovaries and six polycystic ovaries, were collected during transsexual operation and surgical treatment with the signed consent form. The cellular localization of SET protein was observed by immunohistochemistry. The expression levels of SET protein were analyzed by Western Blot. Result. SET protein was expressed predominantly in the theca cells and oocytes of human ovarian follicles in both PCOS ovarian tissues and normal ovarian tissues. The level of SET protein expression in polycystic ovaries was triple higher than that in normal ovaries (P<0.05). Conclusion. SET was overexpressed in polycystic ovaries more than that in normal ovaries. Combined with its localization in theca cells, SET may participate in regulating ovarian androgen biosynthesis and the pathophysiology of hyperandrogenism in PCOS. |
| format | Article |
| id | doaj-art-f869d72a0585459abc406780fa9a6a85 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-f869d72a0585459abc406780fa9a6a852025-02-03T01:30:08ZengWileyInternational Journal of Endocrinology1687-83371687-83452013-01-01201310.1155/2013/367956367956Expression of SET Protein in the Ovaries of Patients with Polycystic Ovary SyndromeXu Boqun0Dai Xiaonan1Cui YuGui2Gao Lingling3Dai Xue4Chao Gao5Diao Feiyang6Liu Jiayin7Li Gao8Mei Li9Yuan Zhang10Xiang Ma11The State Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, ChinaThe State Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, ChinaThe State Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, ChinaThe State Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, ChinaDepartment of Obstetrics and Gynecology, Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, ChinaThe State Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, ChinaThe State Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, ChinaThe State Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, ChinaThe First Affiliated Hospital, Nanjing Medical University, ChinaThe First Affiliated Hospital, Nanjing Medical University, ChinaThe First Affiliated Hospital, Nanjing Medical University, ChinaThe First Affiliated Hospital, Nanjing Medical University, ChinaBackground. We previously found that expression of SET gene was up-regulated in polycystic ovaries by using microarray. It suggested that SET may be an attractive candidate regulator involved in the pathophysiology of polycystic ovary syndrome (PCOS). In this study, expression and cellular localization of SET protein were investigated in human polycystic and normal ovaries. Method. Ovarian tissues, six normal ovaries and six polycystic ovaries, were collected during transsexual operation and surgical treatment with the signed consent form. The cellular localization of SET protein was observed by immunohistochemistry. The expression levels of SET protein were analyzed by Western Blot. Result. SET protein was expressed predominantly in the theca cells and oocytes of human ovarian follicles in both PCOS ovarian tissues and normal ovarian tissues. The level of SET protein expression in polycystic ovaries was triple higher than that in normal ovaries (P<0.05). Conclusion. SET was overexpressed in polycystic ovaries more than that in normal ovaries. Combined with its localization in theca cells, SET may participate in regulating ovarian androgen biosynthesis and the pathophysiology of hyperandrogenism in PCOS.http://dx.doi.org/10.1155/2013/367956 |
| spellingShingle | Xu Boqun Dai Xiaonan Cui YuGui Gao Lingling Dai Xue Chao Gao Diao Feiyang Liu Jiayin Li Gao Mei Li Yuan Zhang Xiang Ma Expression of SET Protein in the Ovaries of Patients with Polycystic Ovary Syndrome International Journal of Endocrinology |
| title | Expression of SET Protein in the Ovaries of Patients with Polycystic Ovary Syndrome |
| title_full | Expression of SET Protein in the Ovaries of Patients with Polycystic Ovary Syndrome |
| title_fullStr | Expression of SET Protein in the Ovaries of Patients with Polycystic Ovary Syndrome |
| title_full_unstemmed | Expression of SET Protein in the Ovaries of Patients with Polycystic Ovary Syndrome |
| title_short | Expression of SET Protein in the Ovaries of Patients with Polycystic Ovary Syndrome |
| title_sort | expression of set protein in the ovaries of patients with polycystic ovary syndrome |
| url | http://dx.doi.org/10.1155/2013/367956 |
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