Evaluation of stability and in vitro wound healing potential of melatonin loaded (lipid enriched) chitosan based microspheres
The aim of this study was to evaluate long-term stability and assess the wound healing potential of the innovative melatonin-loaded lipid-enriched hybrid system compared to conventional melatonin-loaded chitosan microspheres. The hybrid system contained nanostructured lipid carrier incorporated in t...
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| Format: | Article |
| Language: | English |
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Sciendo
2019-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2019-0049 |
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| author | Romić Marieta Duvnjak Sušac Anja Lovrić Jasmina Cetina-Čižmek Biserka Filipović-Grčić Jelena Hafner Anita |
| author_facet | Romić Marieta Duvnjak Sušac Anja Lovrić Jasmina Cetina-Čižmek Biserka Filipović-Grčić Jelena Hafner Anita |
| author_sort | Romić Marieta Duvnjak |
| collection | DOAJ |
| description | The aim of this study was to evaluate long-term stability and assess the wound healing potential of the innovative melatonin-loaded lipid-enriched hybrid system compared to conventional melatonin-loaded chitosan microspheres. The hybrid system contained nanostructured lipid carrier incorporated in the chitosan matrix, in order to modify melatonin release and alter physicochemical characteristics of the delivery system. Stability testing was performed during a six-month period under two conditions: refrigerated (5 ± 3 °C) and at room temperature (25 ± 2 °C/60 ± 5 % RH). Samples stored at both conditions were analyzed in terms of particle size, zeta potential, moisture content and thermal properties. At the end of testing, drug content was determined in all samples. Dressings wound healing potential was assessed by in vitro scratch test using human skin fibroblast cell line. Although both systems showed good stability characteristics, the addition of lipids in the system has improved its wound healing potential. |
| format | Article |
| id | doaj-art-f8455953c0714f36b6d52a82e91a3477 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-f8455953c0714f36b6d52a82e91a34772025-02-02T10:03:15ZengSciendoActa Pharmaceutica1846-95582019-12-0169463564810.2478/acph-2019-0049acph-2019-0049Evaluation of stability and in vitro wound healing potential of melatonin loaded (lipid enriched) chitosan based microspheresRomić Marieta Duvnjak0Sušac Anja1Lovrić Jasmina2Cetina-Čižmek Biserka3Filipović-Grčić Jelena4Hafner Anita5R&D, PLIVA Croatia Ltd, TEVA Group Member, Zagreb, CroatiaUniversity of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, ZagrebCroatiaUniversity of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, ZagrebCroatiaR&D, PLIVA Croatia Ltd, TEVA Group Member, Zagreb, CroatiaUniversity of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, ZagrebCroatiaUniversity of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, ZagrebCroatiaThe aim of this study was to evaluate long-term stability and assess the wound healing potential of the innovative melatonin-loaded lipid-enriched hybrid system compared to conventional melatonin-loaded chitosan microspheres. The hybrid system contained nanostructured lipid carrier incorporated in the chitosan matrix, in order to modify melatonin release and alter physicochemical characteristics of the delivery system. Stability testing was performed during a six-month period under two conditions: refrigerated (5 ± 3 °C) and at room temperature (25 ± 2 °C/60 ± 5 % RH). Samples stored at both conditions were analyzed in terms of particle size, zeta potential, moisture content and thermal properties. At the end of testing, drug content was determined in all samples. Dressings wound healing potential was assessed by in vitro scratch test using human skin fibroblast cell line. Although both systems showed good stability characteristics, the addition of lipids in the system has improved its wound healing potential.https://doi.org/10.2478/acph-2019-0049melatoninnanoparticlesmicrosphereschitosanstabilitywound healing |
| spellingShingle | Romić Marieta Duvnjak Sušac Anja Lovrić Jasmina Cetina-Čižmek Biserka Filipović-Grčić Jelena Hafner Anita Evaluation of stability and in vitro wound healing potential of melatonin loaded (lipid enriched) chitosan based microspheres Acta Pharmaceutica melatonin nanoparticles microspheres chitosan stability wound healing |
| title | Evaluation of stability and in vitro wound healing potential of melatonin loaded (lipid enriched) chitosan based microspheres |
| title_full | Evaluation of stability and in vitro wound healing potential of melatonin loaded (lipid enriched) chitosan based microspheres |
| title_fullStr | Evaluation of stability and in vitro wound healing potential of melatonin loaded (lipid enriched) chitosan based microspheres |
| title_full_unstemmed | Evaluation of stability and in vitro wound healing potential of melatonin loaded (lipid enriched) chitosan based microspheres |
| title_short | Evaluation of stability and in vitro wound healing potential of melatonin loaded (lipid enriched) chitosan based microspheres |
| title_sort | evaluation of stability and in vitro wound healing potential of melatonin loaded lipid enriched chitosan based microspheres |
| topic | melatonin nanoparticles microspheres chitosan stability wound healing |
| url | https://doi.org/10.2478/acph-2019-0049 |
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