The Application of Stress Modifiers as an Eco-Friendly Approach to Alleviate the Water Scarcity in Ajwain (<i>Carum copticum</i> L.) Plants

Stress modifiers are recognized as biostimulants providing beneficial effects on various plant species. However, the specific potential of modulators such as melatonin, chitosan, humic acid, and selenium in enhancing the resistance of ajwain (<i>Carum copticum</i> L.) plants to water sca...

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Main Authors: Saeid Heydarzadeh, Ahmad Tobeh, Sodabeh Jahanbakhsh, Salim Farzaneh, Ermenegilda Vitale, Carmen Arena
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/13/23/3354
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author Saeid Heydarzadeh
Ahmad Tobeh
Sodabeh Jahanbakhsh
Salim Farzaneh
Ermenegilda Vitale
Carmen Arena
author_facet Saeid Heydarzadeh
Ahmad Tobeh
Sodabeh Jahanbakhsh
Salim Farzaneh
Ermenegilda Vitale
Carmen Arena
author_sort Saeid Heydarzadeh
collection DOAJ
description Stress modifiers are recognized as biostimulants providing beneficial effects on various plant species. However, the specific potential of modulators such as melatonin, chitosan, humic acid, and selenium in enhancing the resistance of ajwain (<i>Carum copticum</i> L.) plants to water scarcity remains an open question. To address this knowledge gap, we conducted a randomized, field block-designed factorial experiment over two years (2022–2023) to compare the effectiveness of these biostimulants in mitigating the impact of water shortage on ajwain plants. This study involved three irrigation regimes: 100% field water capacity (FC100%—unstressed), 75% irrigation deficit (FC75%—moderate) and 50% irrigation deficit (FC50%—severe), and four modifier treatments (melatonin, chitosan, humic acid, selenium), plus untreated controls. Plant growth, seed yields, essential oil production, as well as eco-physiological traits were studied to assess the efficacy of these compounds as stress modulators. Water regimes and stress modifier applications, as a single factor or in synergy, significantly affected plant physiology and seed yield, highlighting the importance of sustainability in agricultural practices. Compared to FC100%, biological and seed yield, chlorophyll, and nutrient content decreased under FC75% and FC50%, while essential oil production, proline, soluble sugars, flavonoids, phenols and antioxidant enzymatic activity increased. Notably, regardless of the type of modulator used, the application of these modifiers improved all physiological attributes under moderate and severe irrigation deficits. Among the involved compounds, melatonin induced the most pronounced effects, leading to higher biological and seed yield, essential and fixed oil production, relative leaf water content, chlorophyll and nutrient concentration, and antioxidant activity. Our results demonstrate that such compounds effectively function as stress modulators against water scarcity in ajwain plants by preserving specific eco-physiological traits and promoting water saving. These findings provide valuable insights into their use as a nature-based solution for addressing water stress in sustainable agriculture and climate change challenges.
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spelling doaj-art-f80ca80254da4e2aad83c00e83c38ea52025-08-20T01:55:38ZengMDPI AGPlants2223-77472024-11-011323335410.3390/plants13233354The Application of Stress Modifiers as an Eco-Friendly Approach to Alleviate the Water Scarcity in Ajwain (<i>Carum copticum</i> L.) PlantsSaeid Heydarzadeh0Ahmad Tobeh1Sodabeh Jahanbakhsh2Salim Farzaneh3Ermenegilda Vitale4Carmen Arena5Department of Plant Genetics and Production Engineering, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, P.O. Box 179, IranDepartment of Plant Genetics and Production Engineering, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, P.O. Box 179, IranDepartment of Plant Genetics and Production Engineering, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, P.O. Box 179, IranDepartment of Plant Genetics and Production Engineering, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, P.O. Box 179, IranDepartment of Biology, University of Naples Federico II, 80126 Naples, ItalyDepartment of Biology, University of Naples Federico II, 80126 Naples, ItalyStress modifiers are recognized as biostimulants providing beneficial effects on various plant species. However, the specific potential of modulators such as melatonin, chitosan, humic acid, and selenium in enhancing the resistance of ajwain (<i>Carum copticum</i> L.) plants to water scarcity remains an open question. To address this knowledge gap, we conducted a randomized, field block-designed factorial experiment over two years (2022–2023) to compare the effectiveness of these biostimulants in mitigating the impact of water shortage on ajwain plants. This study involved three irrigation regimes: 100% field water capacity (FC100%—unstressed), 75% irrigation deficit (FC75%—moderate) and 50% irrigation deficit (FC50%—severe), and four modifier treatments (melatonin, chitosan, humic acid, selenium), plus untreated controls. Plant growth, seed yields, essential oil production, as well as eco-physiological traits were studied to assess the efficacy of these compounds as stress modulators. Water regimes and stress modifier applications, as a single factor or in synergy, significantly affected plant physiology and seed yield, highlighting the importance of sustainability in agricultural practices. Compared to FC100%, biological and seed yield, chlorophyll, and nutrient content decreased under FC75% and FC50%, while essential oil production, proline, soluble sugars, flavonoids, phenols and antioxidant enzymatic activity increased. Notably, regardless of the type of modulator used, the application of these modifiers improved all physiological attributes under moderate and severe irrigation deficits. Among the involved compounds, melatonin induced the most pronounced effects, leading to higher biological and seed yield, essential and fixed oil production, relative leaf water content, chlorophyll and nutrient concentration, and antioxidant activity. Our results demonstrate that such compounds effectively function as stress modulators against water scarcity in ajwain plants by preserving specific eco-physiological traits and promoting water saving. These findings provide valuable insights into their use as a nature-based solution for addressing water stress in sustainable agriculture and climate change challenges.https://www.mdpi.com/2223-7747/13/23/3354ajwain plantsnature-based solutionsenvironmental stressstress modulatorssustainable agriculturewater deficit
spellingShingle Saeid Heydarzadeh
Ahmad Tobeh
Sodabeh Jahanbakhsh
Salim Farzaneh
Ermenegilda Vitale
Carmen Arena
The Application of Stress Modifiers as an Eco-Friendly Approach to Alleviate the Water Scarcity in Ajwain (<i>Carum copticum</i> L.) Plants
Plants
ajwain plants
nature-based solutions
environmental stress
stress modulators
sustainable agriculture
water deficit
title The Application of Stress Modifiers as an Eco-Friendly Approach to Alleviate the Water Scarcity in Ajwain (<i>Carum copticum</i> L.) Plants
title_full The Application of Stress Modifiers as an Eco-Friendly Approach to Alleviate the Water Scarcity in Ajwain (<i>Carum copticum</i> L.) Plants
title_fullStr The Application of Stress Modifiers as an Eco-Friendly Approach to Alleviate the Water Scarcity in Ajwain (<i>Carum copticum</i> L.) Plants
title_full_unstemmed The Application of Stress Modifiers as an Eco-Friendly Approach to Alleviate the Water Scarcity in Ajwain (<i>Carum copticum</i> L.) Plants
title_short The Application of Stress Modifiers as an Eco-Friendly Approach to Alleviate the Water Scarcity in Ajwain (<i>Carum copticum</i> L.) Plants
title_sort application of stress modifiers as an eco friendly approach to alleviate the water scarcity in ajwain i carum copticum i l plants
topic ajwain plants
nature-based solutions
environmental stress
stress modulators
sustainable agriculture
water deficit
url https://www.mdpi.com/2223-7747/13/23/3354
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