Effect of 2,4-Thiazolidinedione on Limousin Cattle Growth and on Muscle and Adipose Tissue Metabolism
The main adipogenic transcription factor PPARγ possesses high affinity to 2,4-TZD, a member of the Thiazolidinedione family of insulin-sensitizing compounds used as adipogenic agents. We evaluated 2,4-TZD’s effect on bovine growth and PPAR tissue expression. Seventeen Limousin bulls (18 month-old; 3...
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Wiley
2012-01-01
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| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2012/891841 |
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| author | M. Arévalo-Turrubiarte L. González-Dávalos A. Yabuta J. D. Garza J. L. Dávalos O. Mora A. Shimada |
| author_facet | M. Arévalo-Turrubiarte L. González-Dávalos A. Yabuta J. D. Garza J. L. Dávalos O. Mora A. Shimada |
| author_sort | M. Arévalo-Turrubiarte |
| collection | DOAJ |
| description | The main adipogenic transcription factor PPARγ possesses high affinity to 2,4-TZD, a member of the Thiazolidinedione family of insulin-sensitizing compounds used as adipogenic agents. We evaluated 2,4-TZD’s effect on bovine growth and PPAR tissue expression. Seventeen Limousin bulls (18 month-old; 350 kg body weight (BW)) were assigned into 2 treatments: control and 2,4-TZD (8 mg/70 kg BW) and were fed until bulls reached 500 kg BW. They were weighed and their blood was sampled. DNA, RNA, and protein were determined in liver; skeletal muscle; subcutaneous (SC), omental, perirenal adipose tissues (AT) to determine protein synthesis rate and cellular size. Expression of PPAR mRNA was measured in liver and muscle (PPARα, -δ, and -γ) and SC adipose tissue (γ) by real-time PCR. No significant differences were found (P>0.1) in weight gain, days on feed, and carcass quality. Muscle synthesis was greater in controls (P<0.05); cell size was larger with 2,4-TZD (P<0.05). PPARα, -δ, and -γ expressions with 2,4-TZD in liver were lower (P<0.01) than in muscle. No differences were found for PPARγ mRNA expression in SCAT. The results suggest the potential use of 2,4-TZD in beef cattle diets, because it improves AT differentiation, liver, and muscle fatty acid oxidation that, therefore, might improve energy efficiency. |
| format | Article |
| id | doaj-art-f7f99175c93b44178b4150c3e1018531 |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-f7f99175c93b44178b4150c3e10185312025-02-03T06:01:42ZengWileyPPAR Research1687-47571687-47652012-01-01201210.1155/2012/891841891841Effect of 2,4-Thiazolidinedione on Limousin Cattle Growth and on Muscle and Adipose Tissue MetabolismM. Arévalo-Turrubiarte0L. González-Dávalos1A. Yabuta2J. D. Garza3J. L. Dávalos4O. Mora5A. Shimada6Programa de Posgrado en Ciencias de la Producción y de la Salud Animal, Universidad Nacional Autónoma de México (UNAM), 54714 Mexico City, DF, MexicoLaboratorio de Rumiología y Metabolismo Nutricional (RuMeN), Facultad de Estudios Superiores Cuautitlán (FES Cuautitlán), UNAM, Bulevar B. Quintana 514-D, Colonia Arboledas, 75230 Querétaro, QRO, MexicoFacultad de Medicina Veterinaria y Zootecnia (FMVZ), UNAM and Centro de Enseñanza, Investigación y Extensión en Producción Animal en el Altiplano (CEIEPAA), 76790 Tequisquiapan, QRO, MexicoPrograma de Posgrado en Ciencias de la Producción y de la Salud Animal, Universidad Nacional Autónoma de México (UNAM), 54714 Mexico City, DF, MexicoFacultad de Medicina Veterinaria y Zootecnia (FMVZ), UNAM and Centro de Enseñanza, Investigación y Extensión en Producción Animal en el Altiplano (CEIEPAA), 76790 Tequisquiapan, QRO, MexicoPrograma de Posgrado en Ciencias de la Producción y de la Salud Animal, Universidad Nacional Autónoma de México (UNAM), 54714 Mexico City, DF, MexicoPrograma de Posgrado en Ciencias de la Producción y de la Salud Animal, Universidad Nacional Autónoma de México (UNAM), 54714 Mexico City, DF, MexicoThe main adipogenic transcription factor PPARγ possesses high affinity to 2,4-TZD, a member of the Thiazolidinedione family of insulin-sensitizing compounds used as adipogenic agents. We evaluated 2,4-TZD’s effect on bovine growth and PPAR tissue expression. Seventeen Limousin bulls (18 month-old; 350 kg body weight (BW)) were assigned into 2 treatments: control and 2,4-TZD (8 mg/70 kg BW) and were fed until bulls reached 500 kg BW. They were weighed and their blood was sampled. DNA, RNA, and protein were determined in liver; skeletal muscle; subcutaneous (SC), omental, perirenal adipose tissues (AT) to determine protein synthesis rate and cellular size. Expression of PPAR mRNA was measured in liver and muscle (PPARα, -δ, and -γ) and SC adipose tissue (γ) by real-time PCR. No significant differences were found (P>0.1) in weight gain, days on feed, and carcass quality. Muscle synthesis was greater in controls (P<0.05); cell size was larger with 2,4-TZD (P<0.05). PPARα, -δ, and -γ expressions with 2,4-TZD in liver were lower (P<0.01) than in muscle. No differences were found for PPARγ mRNA expression in SCAT. The results suggest the potential use of 2,4-TZD in beef cattle diets, because it improves AT differentiation, liver, and muscle fatty acid oxidation that, therefore, might improve energy efficiency.http://dx.doi.org/10.1155/2012/891841 |
| spellingShingle | M. Arévalo-Turrubiarte L. González-Dávalos A. Yabuta J. D. Garza J. L. Dávalos O. Mora A. Shimada Effect of 2,4-Thiazolidinedione on Limousin Cattle Growth and on Muscle and Adipose Tissue Metabolism PPAR Research |
| title | Effect of 2,4-Thiazolidinedione on Limousin Cattle Growth and on Muscle and Adipose Tissue Metabolism |
| title_full | Effect of 2,4-Thiazolidinedione on Limousin Cattle Growth and on Muscle and Adipose Tissue Metabolism |
| title_fullStr | Effect of 2,4-Thiazolidinedione on Limousin Cattle Growth and on Muscle and Adipose Tissue Metabolism |
| title_full_unstemmed | Effect of 2,4-Thiazolidinedione on Limousin Cattle Growth and on Muscle and Adipose Tissue Metabolism |
| title_short | Effect of 2,4-Thiazolidinedione on Limousin Cattle Growth and on Muscle and Adipose Tissue Metabolism |
| title_sort | effect of 2 4 thiazolidinedione on limousin cattle growth and on muscle and adipose tissue metabolism |
| url | http://dx.doi.org/10.1155/2012/891841 |
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