Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India
The aim of the present study was to determine the prevalence of Dirofilaria immitis in stray, pet, and working dogs (n=413, 266, and 103, resp.) from Guwahati (Assam) and Aizawl (Mizoram), areas located in two Northeastern States of India. Diagnostic methods applied were microscopy (wet film and Kno...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2015/265385 |
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| author | Sonjoy Kumar Borthakur Dilip Kumar Deka Saidul Islam Dilip Kumar Sarma Prabhat Chandra Sarmah |
| author_facet | Sonjoy Kumar Borthakur Dilip Kumar Deka Saidul Islam Dilip Kumar Sarma Prabhat Chandra Sarmah |
| author_sort | Sonjoy Kumar Borthakur |
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| description | The aim of the present study was to determine the prevalence of Dirofilaria immitis in stray, pet, and working dogs (n=413, 266, and 103, resp.) from Guwahati (Assam) and Aizawl (Mizoram), areas located in two Northeastern States of India. Diagnostic methods applied were microscopy (wet film and Knott’s concentration technique), immunological test (Ag ELISA by SNAP 4Dx ELISA kit), and molecular tools (polymerase chain reaction and sequencing), which evidenced 11.38, 18.03, and 13.93% of positive animals, respectively. No significant differences were observed by area (18.23% versus 17.68%) nor by sex (18.1% versus 17.9%), whereas stray dogs proved more infected than other groups (P<0.05). ELISA test evidenced an overall 22.69% of occult infections, mainly in working dogs (60%), and molecular techniques detected Dirofilaria (Nochtiella) repens in 4 stray dogs from Guwahati. Characterization of D. immitis isolates for ITS-2 region showed close identity with South Asian isolates. |
| format | Article |
| id | doaj-art-f7f788f21ce14409909f74eba3d8110d |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-f7f788f21ce14409909f74eba3d8110d2025-02-03T06:13:58ZengWileyThe Scientific World Journal2356-61401537-744X2015-01-01201510.1155/2015/265385265385Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of IndiaSonjoy Kumar Borthakur0Dilip Kumar Deka1Saidul Islam2Dilip Kumar Sarma3Prabhat Chandra Sarmah4College of Veterinary Sciences & A.H., Central Agricultural University, Selesih, Aizawl, Mizoram 796 014, IndiaCollege of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam 781 022, IndiaCollege of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam 781 022, IndiaNational Research Centre on Pigs (ICAR), Rani, Guwahati, Assam 781 131, IndiaCollege of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam 781 022, IndiaThe aim of the present study was to determine the prevalence of Dirofilaria immitis in stray, pet, and working dogs (n=413, 266, and 103, resp.) from Guwahati (Assam) and Aizawl (Mizoram), areas located in two Northeastern States of India. Diagnostic methods applied were microscopy (wet film and Knott’s concentration technique), immunological test (Ag ELISA by SNAP 4Dx ELISA kit), and molecular tools (polymerase chain reaction and sequencing), which evidenced 11.38, 18.03, and 13.93% of positive animals, respectively. No significant differences were observed by area (18.23% versus 17.68%) nor by sex (18.1% versus 17.9%), whereas stray dogs proved more infected than other groups (P<0.05). ELISA test evidenced an overall 22.69% of occult infections, mainly in working dogs (60%), and molecular techniques detected Dirofilaria (Nochtiella) repens in 4 stray dogs from Guwahati. Characterization of D. immitis isolates for ITS-2 region showed close identity with South Asian isolates.http://dx.doi.org/10.1155/2015/265385 |
| spellingShingle | Sonjoy Kumar Borthakur Dilip Kumar Deka Saidul Islam Dilip Kumar Sarma Prabhat Chandra Sarmah Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India The Scientific World Journal |
| title | Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India |
| title_full | Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India |
| title_fullStr | Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India |
| title_full_unstemmed | Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India |
| title_short | Prevalence and Molecular Epidemiological Data on Dirofilaria immitis in Dogs from Northeastern States of India |
| title_sort | prevalence and molecular epidemiological data on dirofilaria immitis in dogs from northeastern states of india |
| url | http://dx.doi.org/10.1155/2015/265385 |
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