Antibodies from a Patient with Type 1 Diabetes and Celiac Disease Bind to Macrophages that Express the Scavenger Receptor CD163
Antibodies against the wheat storage globulin Glo-3A from a patient with both type 1 diabetes (T1D) and celiac disease were enriched to identify potential molecular mimicry between wheat antigens and T1D target tissues. Recombinant Glo-3A was used to enrich anti-Glo-3A immunoglobulin G antibodies fr...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Canadian Journal of Gastroenterology |
| Online Access: | http://dx.doi.org/10.1155/2011/758579 |
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| _version_ | 1832549903080882176 |
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| author | Brigitte Sonier Alexander Strom Gen-Sheng Wang Christopher Patrick Jennifer A Crookshank Majid Mojibian Amanda J MacFarlane Fraser W Scott |
| author_facet | Brigitte Sonier Alexander Strom Gen-Sheng Wang Christopher Patrick Jennifer A Crookshank Majid Mojibian Amanda J MacFarlane Fraser W Scott |
| author_sort | Brigitte Sonier |
| collection | DOAJ |
| description | Antibodies against the wheat storage globulin Glo-3A from a patient with both type 1 diabetes (T1D) and celiac disease were enriched to identify potential molecular mimicry between wheat antigens and T1D target tissues. Recombinant Glo-3A was used to enrich anti-Glo-3A immunoglobulin G antibodies from plasma by batch affinity chromatography. Rat jejunum and pancreas, as well as human duodenum and monocytes were probed, and binding was evaluated by immunohistochemistry and confocal microscopy. Glo-3A-enriched antibodies bound to a specific subset of cells in the lamina propria of rat jejunum that co-localized mostly with a marker of resident, alternatively activated CD163-positive (CD163+) macrophages. Blood monocytes and macrophage-like cells in human duodenum were also labelled with the enriched antibodies. Blocking studies revealed that binding to CD163+ macrophages was not due to cross-reactivity with anti-Glo-3A antibodies, but rather to non-Glo-3A antibodies co-purified during antibody enrichment. The novel finding of putative autoantibodies against tolerogenic intestinal CD163+ macrophages suggests that regulatory macrophages were targeted in this patient with celiac disease and T1D. |
| format | Article |
| id | doaj-art-f7dfb079d7464322b1cdaeefd9e76806 |
| institution | Kabale University |
| issn | 0835-7900 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Gastroenterology |
| spelling | doaj-art-f7dfb079d7464322b1cdaeefd9e768062025-02-03T06:08:17ZengWileyCanadian Journal of Gastroenterology0835-79002011-01-0125632732910.1155/2011/758579Antibodies from a Patient with Type 1 Diabetes and Celiac Disease Bind to Macrophages that Express the Scavenger Receptor CD163Brigitte Sonier0Alexander Strom1Gen-Sheng Wang2Christopher Patrick3Jennifer A Crookshank4Majid Mojibian5Amanda J MacFarlane6Fraser W Scott7Chronic Disease Program, Ottawa Hospital Research Institute, CanadaChronic Disease Program, Ottawa Hospital Research Institute, CanadaChronic Disease Program, Ottawa Hospital Research Institute, CanadaChronic Disease Program, Ottawa Hospital Research Institute, CanadaChronic Disease Program, Ottawa Hospital Research Institute, CanadaChronic Disease Program, Ottawa Hospital Research Institute, CanadaChronic Disease Program, Ottawa Hospital Research Institute, CanadaChronic Disease Program, Ottawa Hospital Research Institute, CanadaAntibodies against the wheat storage globulin Glo-3A from a patient with both type 1 diabetes (T1D) and celiac disease were enriched to identify potential molecular mimicry between wheat antigens and T1D target tissues. Recombinant Glo-3A was used to enrich anti-Glo-3A immunoglobulin G antibodies from plasma by batch affinity chromatography. Rat jejunum and pancreas, as well as human duodenum and monocytes were probed, and binding was evaluated by immunohistochemistry and confocal microscopy. Glo-3A-enriched antibodies bound to a specific subset of cells in the lamina propria of rat jejunum that co-localized mostly with a marker of resident, alternatively activated CD163-positive (CD163+) macrophages. Blood monocytes and macrophage-like cells in human duodenum were also labelled with the enriched antibodies. Blocking studies revealed that binding to CD163+ macrophages was not due to cross-reactivity with anti-Glo-3A antibodies, but rather to non-Glo-3A antibodies co-purified during antibody enrichment. The novel finding of putative autoantibodies against tolerogenic intestinal CD163+ macrophages suggests that regulatory macrophages were targeted in this patient with celiac disease and T1D.http://dx.doi.org/10.1155/2011/758579 |
| spellingShingle | Brigitte Sonier Alexander Strom Gen-Sheng Wang Christopher Patrick Jennifer A Crookshank Majid Mojibian Amanda J MacFarlane Fraser W Scott Antibodies from a Patient with Type 1 Diabetes and Celiac Disease Bind to Macrophages that Express the Scavenger Receptor CD163 Canadian Journal of Gastroenterology |
| title | Antibodies from a Patient with Type 1 Diabetes and Celiac Disease Bind to Macrophages that Express the Scavenger Receptor CD163 |
| title_full | Antibodies from a Patient with Type 1 Diabetes and Celiac Disease Bind to Macrophages that Express the Scavenger Receptor CD163 |
| title_fullStr | Antibodies from a Patient with Type 1 Diabetes and Celiac Disease Bind to Macrophages that Express the Scavenger Receptor CD163 |
| title_full_unstemmed | Antibodies from a Patient with Type 1 Diabetes and Celiac Disease Bind to Macrophages that Express the Scavenger Receptor CD163 |
| title_short | Antibodies from a Patient with Type 1 Diabetes and Celiac Disease Bind to Macrophages that Express the Scavenger Receptor CD163 |
| title_sort | antibodies from a patient with type 1 diabetes and celiac disease bind to macrophages that express the scavenger receptor cd163 |
| url | http://dx.doi.org/10.1155/2011/758579 |
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