HPV16E6-Dependent c-Fos Expression Contributes to AP-1 Complex Formation in SiHa Cells
To date, the major role of HPV16E6 in cancer has been considered to be its ability to inhibit the p53 tumor-suppressor protein, thereby thwarting p53-mediated cytotoxic responses to cellular stress signals. Here, we show that HPV16E6-dependent c-fos oncogenic protein expression contributes to AP-1 c...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2011/263216 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832559805716234240 |
|---|---|
| author | Feixin Liang Shinichiro Kina Hiroyuki Takemoto Akira Matayoshi Thongsavanh Phonaphonh Nao Sunagawa Keiichi Arakaki Akira Arasaki Hai Kuang Hajime Sunakawa |
| author_facet | Feixin Liang Shinichiro Kina Hiroyuki Takemoto Akira Matayoshi Thongsavanh Phonaphonh Nao Sunagawa Keiichi Arakaki Akira Arasaki Hai Kuang Hajime Sunakawa |
| author_sort | Feixin Liang |
| collection | DOAJ |
| description | To date, the major role of HPV16E6 in cancer has been considered to be its ability to inhibit the p53 tumor-suppressor protein, thereby thwarting p53-mediated cytotoxic responses to cellular stress signals. Here, we show that HPV16E6-dependent c-fos oncogenic protein expression contributes to AP-1 complex formation under oxidative stress in SiHa cells (HPV16-positive squamous cell carcinoma of the cervix). In addition, we examined the role of HPV16E6 in TGF-α-induced c-fos expression and found that the c-fos protein expression induced by TGF-α is HPV16E6 dependent. Thus, our results provide the first evidence that HPV16E6 contributes to AP-1 complex formation after both ligand-dependent and independent EGFR activation, suggesting a new therapeutic approach to the treatment of HPV-associated tumors. |
| format | Article |
| id | doaj-art-f7d3a194791a4e0e9c41848b8998359d |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-f7d3a194791a4e0e9c41848b8998359d2025-02-03T01:29:17ZengWileyMediators of Inflammation0962-93511466-18612011-01-01201110.1155/2011/263216263216HPV16E6-Dependent c-Fos Expression Contributes to AP-1 Complex Formation in SiHa CellsFeixin Liang0Shinichiro Kina1Hiroyuki Takemoto2Akira Matayoshi3Thongsavanh Phonaphonh4Nao Sunagawa5Keiichi Arakaki6Akira Arasaki7Hai Kuang8Hajime Sunakawa9Department of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanDepartment of Oral and Maxillofacial Surgery, Guangxi Medical University, Nanning 530021, Guangxi, ChinaDepartment of Clinical Neuroscience Oral and Maxillofacial Functional Rehabilitation, University of Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, JapanTo date, the major role of HPV16E6 in cancer has been considered to be its ability to inhibit the p53 tumor-suppressor protein, thereby thwarting p53-mediated cytotoxic responses to cellular stress signals. Here, we show that HPV16E6-dependent c-fos oncogenic protein expression contributes to AP-1 complex formation under oxidative stress in SiHa cells (HPV16-positive squamous cell carcinoma of the cervix). In addition, we examined the role of HPV16E6 in TGF-α-induced c-fos expression and found that the c-fos protein expression induced by TGF-α is HPV16E6 dependent. Thus, our results provide the first evidence that HPV16E6 contributes to AP-1 complex formation after both ligand-dependent and independent EGFR activation, suggesting a new therapeutic approach to the treatment of HPV-associated tumors.http://dx.doi.org/10.1155/2011/263216 |
| spellingShingle | Feixin Liang Shinichiro Kina Hiroyuki Takemoto Akira Matayoshi Thongsavanh Phonaphonh Nao Sunagawa Keiichi Arakaki Akira Arasaki Hai Kuang Hajime Sunakawa HPV16E6-Dependent c-Fos Expression Contributes to AP-1 Complex Formation in SiHa Cells Mediators of Inflammation |
| title | HPV16E6-Dependent c-Fos Expression Contributes to AP-1 Complex Formation in SiHa Cells |
| title_full | HPV16E6-Dependent c-Fos Expression Contributes to AP-1 Complex Formation in SiHa Cells |
| title_fullStr | HPV16E6-Dependent c-Fos Expression Contributes to AP-1 Complex Formation in SiHa Cells |
| title_full_unstemmed | HPV16E6-Dependent c-Fos Expression Contributes to AP-1 Complex Formation in SiHa Cells |
| title_short | HPV16E6-Dependent c-Fos Expression Contributes to AP-1 Complex Formation in SiHa Cells |
| title_sort | hpv16e6 dependent c fos expression contributes to ap 1 complex formation in siha cells |
| url | http://dx.doi.org/10.1155/2011/263216 |
| work_keys_str_mv | AT feixinliang hpv16e6dependentcfosexpressioncontributestoap1complexformationinsihacells AT shinichirokina hpv16e6dependentcfosexpressioncontributestoap1complexformationinsihacells AT hiroyukitakemoto hpv16e6dependentcfosexpressioncontributestoap1complexformationinsihacells AT akiramatayoshi hpv16e6dependentcfosexpressioncontributestoap1complexformationinsihacells AT thongsavanhphonaphonh hpv16e6dependentcfosexpressioncontributestoap1complexformationinsihacells AT naosunagawa hpv16e6dependentcfosexpressioncontributestoap1complexformationinsihacells AT keiichiarakaki hpv16e6dependentcfosexpressioncontributestoap1complexformationinsihacells AT akiraarasaki hpv16e6dependentcfosexpressioncontributestoap1complexformationinsihacells AT haikuang hpv16e6dependentcfosexpressioncontributestoap1complexformationinsihacells AT hajimesunakawa hpv16e6dependentcfosexpressioncontributestoap1complexformationinsihacells |