Glycerol Enhances the Antifungal Activity of Dairy Propionibacteria
Dairy propionibacteria are widely used in starter cultures for Swiss type cheese. These bacteria can ferment glucose, lactic acid, and glycerol into propionic acid, acetic acid, and carbon dioxide. This research examined the antifungal effect of dairy propionibacteria when glycerol was used as carbo...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2010/430873 |
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| author | Helena Lind Anders Broberg Karin Jacobsson Hans Jonsson Johan Schnürer |
| author_facet | Helena Lind Anders Broberg Karin Jacobsson Hans Jonsson Johan Schnürer |
| author_sort | Helena Lind |
| collection | DOAJ |
| description | Dairy propionibacteria are widely used in starter cultures for Swiss type cheese. These bacteria can ferment glucose, lactic acid, and glycerol into propionic acid, acetic acid, and carbon dioxide. This research examined the antifungal effect of dairy propionibacteria when glycerol was used as carbon source for bacterial growth. Five type strains of propionibacteria were tested against the yeast Rhodotorula mucilaginosa and the molds Penicillium commune and Penicillium roqueforti. The conversion of 13C glycerol by Propionibacterium jensenii was followed with nuclear magnetic resonance. In a dual culture assay, the degree of inhibition of the molds was strongly enhanced by an increase in glycerol concentrations, while the yeast was less affected. In broth cultures, decreased pH in glycerol medium was probably responsible for the complete inhibition of the indicator fungi. NMR spectra of the glycerol conversion confirmed that propionic acid was the dominant metabolite. Based on the results obtained, the increased antifungal effect seen by glycerol addition to cultures of propionibacteria is due to the production of propionic acid and pH reduction of the medium. |
| format | Article |
| id | doaj-art-f7d2a9033eb849fcadbf5266251171c5 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-f7d2a9033eb849fcadbf5266251171c52025-02-03T01:28:28ZengWileyInternational Journal of Microbiology1687-918X1687-91982010-01-01201010.1155/2010/430873430873Glycerol Enhances the Antifungal Activity of Dairy PropionibacteriaHelena Lind0Anders Broberg1Karin Jacobsson2Hans Jonsson3Johan Schnürer4Department of Microbiology, Swedish University of Agricultural Sciences, P.O. Box 7025, 750 07 Uppsala, SwedenDepartment of Chemistry, Swedish University of Agricultural Sciences, P.O. Box 7015, 750 07 Uppsala, SwedenDepartment of Microbiology, Swedish University of Agricultural Sciences, P.O. Box 7025, 750 07 Uppsala, SwedenDepartment of Microbiology, Swedish University of Agricultural Sciences, P.O. Box 7025, 750 07 Uppsala, SwedenDepartment of Microbiology, Swedish University of Agricultural Sciences, P.O. Box 7025, 750 07 Uppsala, SwedenDairy propionibacteria are widely used in starter cultures for Swiss type cheese. These bacteria can ferment glucose, lactic acid, and glycerol into propionic acid, acetic acid, and carbon dioxide. This research examined the antifungal effect of dairy propionibacteria when glycerol was used as carbon source for bacterial growth. Five type strains of propionibacteria were tested against the yeast Rhodotorula mucilaginosa and the molds Penicillium commune and Penicillium roqueforti. The conversion of 13C glycerol by Propionibacterium jensenii was followed with nuclear magnetic resonance. In a dual culture assay, the degree of inhibition of the molds was strongly enhanced by an increase in glycerol concentrations, while the yeast was less affected. In broth cultures, decreased pH in glycerol medium was probably responsible for the complete inhibition of the indicator fungi. NMR spectra of the glycerol conversion confirmed that propionic acid was the dominant metabolite. Based on the results obtained, the increased antifungal effect seen by glycerol addition to cultures of propionibacteria is due to the production of propionic acid and pH reduction of the medium.http://dx.doi.org/10.1155/2010/430873 |
| spellingShingle | Helena Lind Anders Broberg Karin Jacobsson Hans Jonsson Johan Schnürer Glycerol Enhances the Antifungal Activity of Dairy Propionibacteria International Journal of Microbiology |
| title | Glycerol Enhances the Antifungal Activity of Dairy Propionibacteria |
| title_full | Glycerol Enhances the Antifungal Activity of Dairy Propionibacteria |
| title_fullStr | Glycerol Enhances the Antifungal Activity of Dairy Propionibacteria |
| title_full_unstemmed | Glycerol Enhances the Antifungal Activity of Dairy Propionibacteria |
| title_short | Glycerol Enhances the Antifungal Activity of Dairy Propionibacteria |
| title_sort | glycerol enhances the antifungal activity of dairy propionibacteria |
| url | http://dx.doi.org/10.1155/2010/430873 |
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