The effect of Zn NPs on some growth, biochemical and anatomical factors of chickpea plant stem under UVB irradiation
This study explored the role of zinc oxide nanoparticles (ZnO NPs) in mitigating UV-B radiation effects on chickpea (Cicer arietinum L.) plants. Chickpea plants were grown hydroponically for 45 days and exposed to UV-B radiation for 30 minutes daily over 15 days after reaching the six-leaf stage. Zn...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-05-01
|
| Series: | Plant Nano Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S277311112500021X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850211872519749632 |
|---|---|
| author | Samira Safshekan Latifeh Pourakbar Fatemeh Rahmani |
| author_facet | Samira Safshekan Latifeh Pourakbar Fatemeh Rahmani |
| author_sort | Samira Safshekan |
| collection | DOAJ |
| description | This study explored the role of zinc oxide nanoparticles (ZnO NPs) in mitigating UV-B radiation effects on chickpea (Cicer arietinum L.) plants. Chickpea plants were grown hydroponically for 45 days and exposed to UV-B radiation for 30 minutes daily over 15 days after reaching the six-leaf stage. ZnO NPs were applied at 50 mg/L and 100 mg/L before UV-B exposure. UV-B reduced root length (40 %), shoot fresh weight (17 %), shoot dry weight (15 %), stem thickness (39 %), and pith parenchyma thickness (5 %), while increasing root fresh weight (59 %), root dry weight (12 %), total phenolic content (TPC, 185.77 %), total flavonoid content (TFC, 94.44 %), and DPPH free radical scavenging activity (31.94 %). ZnO NPs (50 mg/L and 100 mg/L) increased root length (15 % and 25 %), shoot length (21 % and 12 %), shoot fresh weight (56 % and 63 %), and shoot dry weight (40 % and 79 %), respectively. TPC increased by 8 % with 50 mg/L ZnO NPs, while TFC rose by 30 % with 100 mg/L ZnO NPs. DPPH activity improved by 15.78 % and 3.59 % at 50 mg/L and 100 mg/L ZnO NPs, respectively. Stem thickness increased by 12 % and 31 %, and pith thickness by 18 % with 50 mg/L ZnO NPs. UV-B reduced sclerenchyma, xylem, and epidermal cell thickness but increased xylem cell length and pith parenchyma, xylem, and epidermal cell width. Application of 100 mg/L ZnO NPs enhanced xylem thickness, phloem vessel thickness, vascular tissue thickness, and collenchyma tissue thickness. Overall, ZnO NPs demonstrated significant potential in alleviating UV-B-induced damage and improving chickpea plant growth and anatomical integrity. |
| format | Article |
| id | doaj-art-f769c6dae01148a5afb8d8e6fff8ea4f |
| institution | OA Journals |
| issn | 2773-1111 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Nano Biology |
| spelling | doaj-art-f769c6dae01148a5afb8d8e6fff8ea4f2025-08-20T02:09:28ZengElsevierPlant Nano Biology2773-11112025-05-011210015410.1016/j.plana.2025.100154The effect of Zn NPs on some growth, biochemical and anatomical factors of chickpea plant stem under UVB irradiationSamira Safshekan0Latifeh Pourakbar1Fatemeh Rahmani2Department of Biology, Faculty of Science, Urmia University, Urmia, IranCorresponding author.; Department of Biology, Faculty of Science, Urmia University, Urmia, IranDepartment of Biology, Faculty of Science, Urmia University, Urmia, IranThis study explored the role of zinc oxide nanoparticles (ZnO NPs) in mitigating UV-B radiation effects on chickpea (Cicer arietinum L.) plants. Chickpea plants were grown hydroponically for 45 days and exposed to UV-B radiation for 30 minutes daily over 15 days after reaching the six-leaf stage. ZnO NPs were applied at 50 mg/L and 100 mg/L before UV-B exposure. UV-B reduced root length (40 %), shoot fresh weight (17 %), shoot dry weight (15 %), stem thickness (39 %), and pith parenchyma thickness (5 %), while increasing root fresh weight (59 %), root dry weight (12 %), total phenolic content (TPC, 185.77 %), total flavonoid content (TFC, 94.44 %), and DPPH free radical scavenging activity (31.94 %). ZnO NPs (50 mg/L and 100 mg/L) increased root length (15 % and 25 %), shoot length (21 % and 12 %), shoot fresh weight (56 % and 63 %), and shoot dry weight (40 % and 79 %), respectively. TPC increased by 8 % with 50 mg/L ZnO NPs, while TFC rose by 30 % with 100 mg/L ZnO NPs. DPPH activity improved by 15.78 % and 3.59 % at 50 mg/L and 100 mg/L ZnO NPs, respectively. Stem thickness increased by 12 % and 31 %, and pith thickness by 18 % with 50 mg/L ZnO NPs. UV-B reduced sclerenchyma, xylem, and epidermal cell thickness but increased xylem cell length and pith parenchyma, xylem, and epidermal cell width. Application of 100 mg/L ZnO NPs enhanced xylem thickness, phloem vessel thickness, vascular tissue thickness, and collenchyma tissue thickness. Overall, ZnO NPs demonstrated significant potential in alleviating UV-B-induced damage and improving chickpea plant growth and anatomical integrity.http://www.sciencedirect.com/science/article/pii/S277311112500021XCicer arietinumCells lengthDPPH free radical scavengingNanoparticlesPhenolic compoundsUltraviolet ray |
| spellingShingle | Samira Safshekan Latifeh Pourakbar Fatemeh Rahmani The effect of Zn NPs on some growth, biochemical and anatomical factors of chickpea plant stem under UVB irradiation Plant Nano Biology Cicer arietinum Cells length DPPH free radical scavenging Nanoparticles Phenolic compounds Ultraviolet ray |
| title | The effect of Zn NPs on some growth, biochemical and anatomical factors of chickpea plant stem under UVB irradiation |
| title_full | The effect of Zn NPs on some growth, biochemical and anatomical factors of chickpea plant stem under UVB irradiation |
| title_fullStr | The effect of Zn NPs on some growth, biochemical and anatomical factors of chickpea plant stem under UVB irradiation |
| title_full_unstemmed | The effect of Zn NPs on some growth, biochemical and anatomical factors of chickpea plant stem under UVB irradiation |
| title_short | The effect of Zn NPs on some growth, biochemical and anatomical factors of chickpea plant stem under UVB irradiation |
| title_sort | effect of zn nps on some growth biochemical and anatomical factors of chickpea plant stem under uvb irradiation |
| topic | Cicer arietinum Cells length DPPH free radical scavenging Nanoparticles Phenolic compounds Ultraviolet ray |
| url | http://www.sciencedirect.com/science/article/pii/S277311112500021X |
| work_keys_str_mv | AT samirasafshekan theeffectofznnpsonsomegrowthbiochemicalandanatomicalfactorsofchickpeaplantstemunderuvbirradiation AT latifehpourakbar theeffectofznnpsonsomegrowthbiochemicalandanatomicalfactorsofchickpeaplantstemunderuvbirradiation AT fatemehrahmani theeffectofznnpsonsomegrowthbiochemicalandanatomicalfactorsofchickpeaplantstemunderuvbirradiation AT samirasafshekan effectofznnpsonsomegrowthbiochemicalandanatomicalfactorsofchickpeaplantstemunderuvbirradiation AT latifehpourakbar effectofznnpsonsomegrowthbiochemicalandanatomicalfactorsofchickpeaplantstemunderuvbirradiation AT fatemehrahmani effectofznnpsonsomegrowthbiochemicalandanatomicalfactorsofchickpeaplantstemunderuvbirradiation |