Investigation of small ruminant lentivirus infections by serological and molecular methods in eastern mediterranean region
<b>Aim: </b>The aim of this study was to investigate the presence of small ruminant lentivirus infections in samples taken from goat and sheep farms.<p> <b>Materials and Methods: </b>In this study, leukocyte and blood serum samples were taken from a total of 700 animals...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1364 |
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| Summary: | <b>Aim: </b>The aim of this study was to investigate the presence of small ruminant
lentivirus infections in samples taken from goat and sheep farms.<p>
<b>Materials and Methods: </b>In this study, leukocyte and blood serum samples
were taken from a total of 700 animals, including 260 sheep and 440
goats aged 6 months and over, from public-owned goats and sheep farms
collected from the Eastern Mediterranean region (Hatay, Kahramanmaraş
and Osmaniye) between 2015-2017 year. Serologic (ELISA) and molecular
method (PCR) tests were used to reveal the presence of small ruminant
lentivirus.<p>
<b>Results:</b> According to the results of ELISA and / or PCR tests, positivity
was detected at a rate of 9.62% (25/260) in sheep, 9.32% (41/440) in goats
and 9.43% (66/700) in all animals. In addition, as a result of the partial
sequence analysis of the gag gene region, the presence of the B genotype of
small ruminant lentivirus was detected in both sheep and goats.<p>
<b>Conclusion:</b> As a result, the situation of small ruminant lentiviruses in the
Eastern Mediterranean (Hatay, Kahramanmaraş, Osmaniye) region in the
recent past has been revealed. In this study, it was concluded that ELISA
and PCR tests should be used together for the diagnosis of small ruminant
lentivirus infections and complete herd-based eradication. In addition, it
may be important to increase molecular studies, to reveal different subtypes
by conducting detailed researches on different gene regions and full
genome analysis, thus determining the subtypes circulating in the country. |
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| ISSN: | 1309-6958 2146-1953 |