Body composition, mitochondrial oxygen metabolism and metabolome of patients with obesity before and after bariatric surgery (COMMITMENT): protocol for a monocentric prospective cohort study
Introduction Obesity, defined as a body mass index ≥30 kg/m2, is one of the most prevalent health conditions worldwide. It is part of the metabolic syndrome, which encompasses arterial hypertension, dyslipoproteinaemia and diabetes. Obesity is viewed as a systemic disease with pathophysiological mec...
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| Format: | Article |
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BMJ Publishing Group
2022-06-01
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| Series: | BMJ Open |
| Online Access: | https://bmjopen.bmj.com/content/12/6/e062592.full |
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| author | Sina M Coldewey Charles Neu Philipp Baumbach Utz Settmacher Kornel Skitek Hermann Kissler Ricardo Esper Treml |
| author_facet | Sina M Coldewey Charles Neu Philipp Baumbach Utz Settmacher Kornel Skitek Hermann Kissler Ricardo Esper Treml |
| author_sort | Sina M Coldewey |
| collection | DOAJ |
| description | Introduction Obesity, defined as a body mass index ≥30 kg/m2, is one of the most prevalent health conditions worldwide. It is part of the metabolic syndrome, which encompasses arterial hypertension, dyslipoproteinaemia and diabetes. Obesity is viewed as a systemic disease with pathophysiological mechanisms on the molecular level. Dysfunction of the mitochondrion and systemic low-grade inflammation are among the proposed causes for the metabolic changes. In severe cases of obesity, laparoscopic sleeve gastrectomy, a bariatric operation, can achieve the desired weight loss and has been associated with clinical outcome improvement. Hitherto, the influence of patients’ body composition on mitochondrial function and concomitant metabolic changes has not been fully understood. This study aims to quantify the patient’s body composition before and after laparoscopic sleeve gastrectomy and to correlate these findings with changes in mitochondrial oxygen metabolism, metabolome and immune status.Methods and analysis In this prospective monocentric cohort study, patients undergoing laparoscopic sleeve gastrectomy (n=30) at Jena University Hospital (Germany) will be assessed before surgery and at four time points during a 1-year follow-up. Body composition will be measured by bioimpedance analysis. Non-invasive assessment of mitochondrial oxygen metabolism using protoporphyrin IX-triplet state lifetime technique (PPIX-TSLT) and blood sampling for, among other, metabolomic and immunological analysis, will be performed. The primary outcome is the difference in relative fat mass between the preoperative time point and 6 months postoperatively. Further outcomes comprise longitudinal changes of PPIX-TSLT and metabolic and immunological variables. Outcomes will be assessed using paired t-tests, Wilcoxon signed-rank tests and regression analyses.Ethics and dissemination The study was approved by the Ethics Committee of Friedrich Schiller University Jena (2018-1192-BO). Written informed consent will be obtained from all patients prior to enrolment in the study. The results will be published in peer-reviewed journals and presented at appropriate conferences.Trial registration number DRKS00015891. |
| format | Article |
| id | doaj-art-f73ab475379a4651ae306b28667b4659 |
| institution | Kabale University |
| issn | 2044-6055 |
| language | English |
| publishDate | 2022-06-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | BMJ Open |
| spelling | doaj-art-f73ab475379a4651ae306b28667b46592025-02-01T14:40:09ZengBMJ Publishing GroupBMJ Open2044-60552022-06-0112610.1136/bmjopen-2022-062592Body composition, mitochondrial oxygen metabolism and metabolome of patients with obesity before and after bariatric surgery (COMMITMENT): protocol for a monocentric prospective cohort studySina M Coldewey0Charles Neu1Philipp Baumbach2Utz Settmacher3Kornel Skitek4Hermann Kissler5Ricardo Esper Treml6Septomics Research Centre, Jena University Hospital, Jena, GermanySeptomics Research Centre, Jena University Hospital, Jena, GermanyDepartment of Anaesthesiology and Intensive Care Medicine, Jena University Hospital, Jena, GermanyDepartment of General, Visceral and Vascular Surgery, Friedrich-Schiller University, Jena, GermanyDepartment of Anaesthesiology and Intensive Care Medicine, Jena University Hospital, Jena, GermanyDepartment of General, Visceral and Vascular Surgery, Jena University Hospital, Jena, GermanyDepartment of Anaesthesiology and Intensive Care Medicine, Jena University Hospital, Jena, GermanyIntroduction Obesity, defined as a body mass index ≥30 kg/m2, is one of the most prevalent health conditions worldwide. It is part of the metabolic syndrome, which encompasses arterial hypertension, dyslipoproteinaemia and diabetes. Obesity is viewed as a systemic disease with pathophysiological mechanisms on the molecular level. Dysfunction of the mitochondrion and systemic low-grade inflammation are among the proposed causes for the metabolic changes. In severe cases of obesity, laparoscopic sleeve gastrectomy, a bariatric operation, can achieve the desired weight loss and has been associated with clinical outcome improvement. Hitherto, the influence of patients’ body composition on mitochondrial function and concomitant metabolic changes has not been fully understood. This study aims to quantify the patient’s body composition before and after laparoscopic sleeve gastrectomy and to correlate these findings with changes in mitochondrial oxygen metabolism, metabolome and immune status.Methods and analysis In this prospective monocentric cohort study, patients undergoing laparoscopic sleeve gastrectomy (n=30) at Jena University Hospital (Germany) will be assessed before surgery and at four time points during a 1-year follow-up. Body composition will be measured by bioimpedance analysis. Non-invasive assessment of mitochondrial oxygen metabolism using protoporphyrin IX-triplet state lifetime technique (PPIX-TSLT) and blood sampling for, among other, metabolomic and immunological analysis, will be performed. The primary outcome is the difference in relative fat mass between the preoperative time point and 6 months postoperatively. Further outcomes comprise longitudinal changes of PPIX-TSLT and metabolic and immunological variables. Outcomes will be assessed using paired t-tests, Wilcoxon signed-rank tests and regression analyses.Ethics and dissemination The study was approved by the Ethics Committee of Friedrich Schiller University Jena (2018-1192-BO). Written informed consent will be obtained from all patients prior to enrolment in the study. The results will be published in peer-reviewed journals and presented at appropriate conferences.Trial registration number DRKS00015891.https://bmjopen.bmj.com/content/12/6/e062592.full |
| spellingShingle | Sina M Coldewey Charles Neu Philipp Baumbach Utz Settmacher Kornel Skitek Hermann Kissler Ricardo Esper Treml Body composition, mitochondrial oxygen metabolism and metabolome of patients with obesity before and after bariatric surgery (COMMITMENT): protocol for a monocentric prospective cohort study BMJ Open |
| title | Body composition, mitochondrial oxygen metabolism and metabolome of patients with obesity before and after bariatric surgery (COMMITMENT): protocol for a monocentric prospective cohort study |
| title_full | Body composition, mitochondrial oxygen metabolism and metabolome of patients with obesity before and after bariatric surgery (COMMITMENT): protocol for a monocentric prospective cohort study |
| title_fullStr | Body composition, mitochondrial oxygen metabolism and metabolome of patients with obesity before and after bariatric surgery (COMMITMENT): protocol for a monocentric prospective cohort study |
| title_full_unstemmed | Body composition, mitochondrial oxygen metabolism and metabolome of patients with obesity before and after bariatric surgery (COMMITMENT): protocol for a monocentric prospective cohort study |
| title_short | Body composition, mitochondrial oxygen metabolism and metabolome of patients with obesity before and after bariatric surgery (COMMITMENT): protocol for a monocentric prospective cohort study |
| title_sort | body composition mitochondrial oxygen metabolism and metabolome of patients with obesity before and after bariatric surgery commitment protocol for a monocentric prospective cohort study |
| url | https://bmjopen.bmj.com/content/12/6/e062592.full |
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