Effects of copper and zinc on proteoglycan metabolism in articular cartilage
Co-Cultures of porcine articular cartilage and synovium or synovial conditioned medium were used as an in vitro model to mimic inflammatory events at the cartilage/synovial junction in degenerative joint disease. This model provides a useful tool to assess the anti-inflammatory and antiarthritic pro...
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| Format: | Article |
| Language: | English |
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Wiley
1996-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935196000154 |
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| author | M. Pasqualicchio R. Gasperini G. P. Velo M. E. Davies |
| author_facet | M. Pasqualicchio R. Gasperini G. P. Velo M. E. Davies |
| author_sort | M. Pasqualicchio |
| collection | DOAJ |
| description | Co-Cultures of porcine articular cartilage and synovium or synovial conditioned medium were used as an in vitro model to mimic inflammatory events at the cartilage/synovial junction in degenerative joint disease. This model provides a useful tool to assess the anti-inflammatory and antiarthritic properties of pharmacological agents. In this study the effects of copper and zinc on (i) PG synthesis by cartilage and (ii) synovial-induced PG depletion have been investigated. Copper sulphate at a concentration of 0.01 mM did not stimulate PG synthesis significantly in cultured cartilage explants but completely abrogated the inhibitory effects of synovial tissue in co-culture experiments. This finding was supported by the histological demonstration of copper-dependent reversal of the PG depletion in cartilage exposed to synovial conditioned medium. Zinc sulphate at 0.01 mM had no effect on PG synthesis and was unable to protect cartilage against synovialinduced PG depletion. These results reveal possible mechanisms by which copper exerts its anti-inflammatory and anti-arthritic actions. |
| format | Article |
| id | doaj-art-f7281a0d33ea4558b7751a9fab10ef05 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1996-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-f7281a0d33ea4558b7751a9fab10ef052025-02-03T06:08:00ZengWileyMediators of Inflammation0962-93511466-18611996-01-0152959910.1155/S0962935196000154Effects of copper and zinc on proteoglycan metabolism in articular cartilageM. Pasqualicchio0R. Gasperini1G. P. Velo2M. E. Davies3Strangeways Research Laboratory, Worts' Causeway, Cambridge CB1 4RN, UKInstitute of Pharmacology, University of Verona, Verona, ItalyInstitute of Pharmacology, University of Verona, Verona, ItalyStrangeways Research Laboratory, Worts' Causeway, Cambridge CB1 4RN, UKCo-Cultures of porcine articular cartilage and synovium or synovial conditioned medium were used as an in vitro model to mimic inflammatory events at the cartilage/synovial junction in degenerative joint disease. This model provides a useful tool to assess the anti-inflammatory and antiarthritic properties of pharmacological agents. In this study the effects of copper and zinc on (i) PG synthesis by cartilage and (ii) synovial-induced PG depletion have been investigated. Copper sulphate at a concentration of 0.01 mM did not stimulate PG synthesis significantly in cultured cartilage explants but completely abrogated the inhibitory effects of synovial tissue in co-culture experiments. This finding was supported by the histological demonstration of copper-dependent reversal of the PG depletion in cartilage exposed to synovial conditioned medium. Zinc sulphate at 0.01 mM had no effect on PG synthesis and was unable to protect cartilage against synovialinduced PG depletion. These results reveal possible mechanisms by which copper exerts its anti-inflammatory and anti-arthritic actions.http://dx.doi.org/10.1155/S0962935196000154 |
| spellingShingle | M. Pasqualicchio R. Gasperini G. P. Velo M. E. Davies Effects of copper and zinc on proteoglycan metabolism in articular cartilage Mediators of Inflammation |
| title | Effects of copper and zinc on proteoglycan metabolism in articular cartilage |
| title_full | Effects of copper and zinc on proteoglycan metabolism in articular cartilage |
| title_fullStr | Effects of copper and zinc on proteoglycan metabolism in articular cartilage |
| title_full_unstemmed | Effects of copper and zinc on proteoglycan metabolism in articular cartilage |
| title_short | Effects of copper and zinc on proteoglycan metabolism in articular cartilage |
| title_sort | effects of copper and zinc on proteoglycan metabolism in articular cartilage |
| url | http://dx.doi.org/10.1155/S0962935196000154 |
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